Objective To compare the clinical therapeutic effects of robot-assisted and conventional thoracotomy with mitral valvuloplasty, and to further clarify the advantages and disadvantages of the Da Vinci robotic surgery system in cardiac surgery. Method A total of 116 patients with mitral valvuloplasty were enrolled from November 2014 to July 2018 in the Affiliated Hospital of Qingdao University, which included 38 cases of the robot-assisted mitral valvuloplasty and 78 cases of conventional thoracotomy with mitral valvuloplasty; the clinical treatment of the two patient groups was compared and analyzed. Result The surgical outcomes of the two groups were satisfactory and there were no deaths in the hospital. The operation time, cardiopulmonary bypass (CPB) time, and ascending aorta occlusion time were longer in the Da Vinci group than in the conventional group (P<0.05). Intensive care unit(ICU) time, tracheal intubation time, postoperative hospital stay, postoperative blood transfusion, postoperative drainage and incidence of postoperative complications were lower in the Da Vinci group than in the conventional group (P<0.05). There was no significant difference in postoperative cardiac ultrasound results between the Da Vinci group and the conventional group. (P>0.05). Conclusion Robotic technique can be safely and effectively be applied in mitral valvuloplasty, and can significantly shorten the ICU time, tracheal intubation time and postoperative hospital stay, also reduce postoperative blood transfusion, postoperative drainage, and incidence of postoperative complications. Robot-assisted surgery is a good choice for minimally invasive surgery, but its operation time, extracorporeal circulation time and ascending aorta occlusion time are longer than conventional surgery, and further improvement is needed.