Latency and inhibitability by metabolites of glucose-6-phosphatase of permeable hepatocytes from fasted and fed rats.

Nordlie, Robert C.; Jorgenson, Roger A.

doi:10.1016/s0021-9258(19)69318-x

Cited by 21 publications

References 24 publications

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Endoplasmic Reticulum

B��nhegyi¹,

Benedetti

Csala

2008

Wiley Encyclopedia of Chemical Biology

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Several pathways are compartmentalized in the endoplasmic reticulum (ER). These intraluminal activities require the passage of substrates, cofactors, and products through the ER membrane. The arguments for a general permeability of the ER membrane are contradicted by strong biochemical, pharmacological, clinical, and genetic evidence, which indicates that the lipid bilayer has a barrier function and that specific transport activities are needed in the membrane. Consequently, the ER lumen can be regarded as a separate metabolic compartment. This article overviews the best characterized intraluminal processes in which the compartmentation is important either by defining an intraluminal milieu, by limiting the rate of the reaction, by determining the specificity, or by creating a common substrate pool because of the colocalization.

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Endoplasmic Reticulum

B��nhegyi¹,

Benedetti

Csala

2008

Wiley Encyclopedia of Chemical Biology

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A critical evaluation of the use of filipin‐permeabilized rat hepatocytes to study functions of the endoplasmic reticulum in situ

McEwen

Telford

Handelman

et al. 1987

Cell Biochemistry & Function

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We have used transmission (TEM) and scanning electron microscopy (SEM) and leakage of lactate dehydrogenase (LDH; EC 1.1.1.27) to evaluate two published procedures which use filipin to render isolated rat hepatocytes permeable to ionic substrates. Cells treated by the procedure of Jorgenson and Nordlie retained less than 10 per cent of their LDH. TEM revealed severe damage to the internal structure of these cells, which included swelling, disintegration and extensive vesicularization of the endoplasmic reticulum (ER). Hepatocytes treated with filipin by the procedure of Gankema et al. retained 65-75 per cent of their LDH and displayed incomplete but highly variable permeability to Trypan blue. SEM revealed the loss of microvilli, other signs of swelling, and the presence of large lesions in the plasma membrane. TEM revealed signs of cell swelling, but the nuclei and the mitochondria were only moderately altered. The rough ER was not swollen, but significant fragmentation was evident and characteristic stacks of lamellar ER were never seen. We conclude that useful information about the functions of the ER in situ cannot be obtained from studies of filipin-treated cells. Our results indicate that retention of LDH is not a sufficient criterion of preservation of cell morphology and that staining with Trypan blue may significantly underestimate the permeability of cells to small ionic metabolites.

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Multifunctional Glucose-6-Phosphatase: A Critical Review

Nordlie

Sukalski

1985

The Enzymes of Biological Membranes

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Latency and inhibitability by metabolites of glucose-6-phosphatase of permeable hepatocytes from fasted and fed rats.

Cited by 21 publications

References 24 publications

Endoplasmic Reticulum

Endoplasmic Reticulum

A critical evaluation of the use of filipin‐permeabilized rat hepatocytes to study functions of the endoplasmic reticulum in situ

Multifunctional Glucose-6-Phosphatase: A Critical Review

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