Later Evaluation of Ischemia and Reperfusion by the Pringle Maneuver in Wistar Rats, Demonstrating That Hepatic Lesions Can Be Reversible

Jorge, Gracinda de Lourdes; Tártaro, Rodolfo dos Reis; Escanhoela, C.A.F.; Boin, I.F.S.F.

doi:10.1016/j.transproceed.2017.01.054

Cited by 4 publications

(6 citation statements)

References 13 publications

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“…Delourdes and colleagues [53] have reported that histopathological changes associated with 20 min of hepatic IRI animal models were completely reversed in the postoperative 28th day. The current research on hepatic IRI is performed on experimental models that performed ischemia between 15 to 60 min [52][53][54][55][56]. In our study, we have performed histopathologic analyses on the liver grafts harvested with and without the Pringle maneuver and liver specimens were obtained during the "Back-Table " procedures.…”

Section: Discussionmentioning

confidence: 98%

“…Determination of hepatic IRI is difficult. The morphologic findings of hepatic IRI have been reported as eosinophilia, pyknosis, chromatin fragmentation, steatosis, and vacuolization [52,53]. Delourdes and colleagues [53] have reported that histopathological changes associated with 20 min of hepatic IRI animal models were completely reversed in the postoperative 28th day.…”

Section: Discussionmentioning

confidence: 99%

“…The morphologic findings of hepatic IRI have been reported as eosinophilia, pyknosis, chromatin fragmentation, steatosis, and vacuolization [52,53]. Delourdes and colleagues [53] have reported that histopathological changes associated with 20 min of hepatic IRI animal models were completely reversed in the postoperative 28th day. The current research on hepatic IRI is performed on experimental models that performed ischemia between 15 to 60 min [52][53][54][55][56].…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Pringle Maneuver Applied during Living Donor Hepatectomy on the Ischemia-Reperfusion Injury Observed in the Donors and Recipients

Dalda,

Akbulut,

Sahin

et al. 2024

Medicina

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Background and Objectives: The aim of this study is to evaluate the clinical and laboratory changes of ischemia and reperfusion injury in the remnant livers of donors with and without Pringle maneuver. Furthermore, we evaluated the recipients who have been transplanted with liver grafts from these donors. Methods and Materials: A total of 108 patients (54 living liver donors and 54 liver recipients) who underwent donor hepatectomy and recipients who living donor liver transplantation, were included in this randomized double-blind study between February 2021 and June 2021. The donors were divided into two groups: Pringle maneuver applied (n = 27) and Pringle maneuver not applied (n = 27). Similarly, recipients with implanted liver obtained from these donors were divided into two groups as the Pringle maneuver was performed (n = 27) and not performed (n = 27). Blood samples from donors and recipients were obtained on pre-operative, post-operative 0 h day (day of surgery), post-operative 1st day, post-operative 2nd day, post-operative 3rd day, post-operative 4th day, post-operative 5th day, and liver tissue was taken from the graft during the back table procedures. Liver function tests and complete blood count, coagulation tests, IL-1, IL-2, IL-6, TNF-α, and β-galactosidase measurements, and histopathological findings were examined. Results: There was no statistically significant difference in the parameters of biochemical analyses for ischemia-reperfusion injury at all periods in the donors with and without the Pringle maneuver. Similarly, there was no statistically significant difference between in the recipients in who received liver grafts harvested with and without the Pringle maneuver. There was no statistically significant difference between the two recipient groups in terms of perioperative bleeding and early bile duct complications (p = 0.685). In the histopathological examinations, hepatocyte damage was significantly higher in the Pringle maneuver group (p = 0.001). Conclusions: Although the histological scoring of hepatocyte damage was found to be higher in the Pringle maneuver group, the Pringle maneuver did not augment ischemia-reperfusion injury in donors and recipients that was evaluated by clinical and laboratory analyses.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The Effect of Pringle Maneuver Applied during Living Donor Hepatectomy on the Ischemia-Reperfusion Injury Observed in the Donors and Recipients

Dalda,

Akbulut,

Sahin

et al. 2024

Medicina

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“…In the literature, the reversal of the mitochondrial lesion in the 20-minute ischemia has been described; the groups were evaluated immediately and 20 minutes after reperfusion. Improvement in mitochondrial respiration and morphological recovery directly related to the type and intensity of the lesion 24 , were observed. The surgical technique also includes hypothermic perfusion and parenchymal hypothermia, capable of reducing the inflammatory response and increasing ischemic tolerance 25 .…”

Section: Cheating the Ir Injurymentioning

confidence: 89%

Liver ischemia and reperfusion injury. Pathophysiology and new horizons in preconditioning and therapy

Nakazato

Victorino²,

Fina³

et al. 2018

Acta Cir. Bras.

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It is well known that during hepatic operative procedures, it is often critical that the irrigation is interrupted to avoid possible bleeding, blood transfusions, variable intensities, and their short and long-term consequences. It was believed in the past that the flow interruption should not exceed 20 minutes, which limited the use of this maneuver. However, it has been postulated that ischemia could be maintained for more than 60 minutes in healthy livers. The present paper review includes: 1) A brief introduction to justify the rationale of the review design; 2) Aspects of the pathophysiology of the three stages of the liver ischemia-reperfusion injury; 3) The innate and acquired immunity; 4) Oxidative stress; 5) Apoptosis and autophagy, Some essential biomarkers (Tumor Necrosis Factor-α, nitric oxide, metalloproteinases); and, finally; 6) Preventive ("cheating") strategies, non-pharmacological and pharmacological options to treat the liver IR injury.

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“…Durante isquemia normotérmica pode ocorrer vacuolização citoplasmática, congestão sinusoidal e/ou venosa, balonização e dependendo do tempo de isquemia, focos de necrose e infiltração gordurosa 1,11,12,27,32,112 .…”

Section: Introductionunclassified

Avaliação da recuperação das lesões hepáticas, através da histologia, bioquímica e da respiração mitocondrial, após manobra de Pringle em diferentes períodos de isquemia e reperfusão, em ratos Wistar

Tártaro¹

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Agradeço, em primeiro lugar, a Deus, ao Mestre Jesus, aos bons espíritos e ao meu anjo guardião que me iluminaram, encaminharam, fornecendo o equilíbrio, a força e a coragem necessária durante toda esta minha jornada.Aos meus pais, Paulo Tártaro e Zenaide dos Reis Tártaro que, com muito carinho e amor, não mediram esforços para que eu concluísse esta etapa de minha vida.À minha irmã, Camila dos Reis Tártaro Ishibashi e ao meu cunhado, Márcio Yoshio Ishibashi, que me deram uma gigantesca oportunidade de estudar, amar, sonhar e poder concretizar mais esta etapa de minha vida, através de seus exemplos.À minha orientadora, Professora Doutora Ilka de Fátima Santana Ferreira Boin, por acreditar em meus esforços, pela atenção, carinho e por me guiar durante esta construção de conhecimento.À Doutora Gracinda de Lourdes Jorge, pelos 14 anos de dedicação em me conduzir ao fascinante universo da pesquisa científica, pela sua preciosa amizade conquistada durante todos estes anos de muito aprendizado, incentivo, carinho, paciência, companheirismo, me mostrando o caminho correto e ético, me tornando assim, um profissional e uma pessoa melhor.À Professora Doutora Cecília Amélia Fazzio Escanhoela, pela grande assessoria durante as avaliações histológicas.Ao Professor Doutor Luiz Sérgio Leonardi, por abrir as portas do Laboratório de Transplante Hepático Experimental do NMCE durante minha graduação.Ao Professor Doutor Aníbal Eugenio Vercesi, que abriu as portas do Laboratório de Bioenergética do NMCE e confiou em nosso potencial e em nosso trabalho.Ao Professor Doutor Roger Frigério Castilho, que possibilitou o meu aprimoramento e crescimento durante meus estudos.À farmacêutica bioquímica, Ana Cândida Coutinho Facin, pela colaboração nas avaliações bioquímicas.Ao Professor Mestre Antônio Henrique Dianin, pela amizade e a oportunidade de trabalharmos juntos, abrindo assim, novos horizontes e possibilidades de estudos.Ao Professor Doutor Konradin Metze e a Rosana Trevisan do Laboratório de Anatomia Patológica Experimental do NMCE, pela amizade, incentivo e contribuição na confecção das lâminas para avaliação histopatológica.À Laura de Brito Évora, que vem participando de toda esta trajetória me dando carinho, apoio, amizade, respeito, ensinamentos e muito amor durante esta minha jornada.À Coordenação de Aperfeiçoamento Pessoal de Nível Superior -Brasil (CAPES), pela bolsa de estudos que possibilitou a dedicação integral ao programa de pós-graduação durante os meus estudos, com código de financiamento 001.Aos meus 50 irmãos de república, que me apoiaram sempre com suas brincadeiras, atitudes, ensinamentos e alegrias durante os bons e não tão bons momentos.Ao meu irmão, Leopoldo dos Reis Tártaro, (in memoriam) por me dar bons exemplos, sempre me aconselhando, me mostrando que devemos batalhar por aquilo que acreditamos e sempre demonstrando seu grande amor e carinho com todos ao seu redor.

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Later Evaluation of Ischemia and Reperfusion by the Pringle Maneuver in Wistar Rats, Demonstrating That Hepatic Lesions Can Be Reversible

Abstract: The oxidative phosphorylation system recovered with improvement in mitochondrial respiration; however, morphologic recovery was associated with the type and intensity of injury.

Cited by 4 publications

References 13 publications

The Effect of Pringle Maneuver Applied during Living Donor Hepatectomy on the Ischemia-Reperfusion Injury Observed in the Donors and Recipients

The Effect of Pringle Maneuver Applied during Living Donor Hepatectomy on the Ischemia-Reperfusion Injury Observed in the Donors and Recipients

Liver ischemia and reperfusion injury. Pathophysiology and new horizons in preconditioning and therapy

Avaliação da recuperação das lesões hepáticas, através da histologia, bioquímica e da respiração mitocondrial, após manobra de Pringle em diferentes períodos de isquemia e reperfusão, em ratos Wistar

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