2015
DOI: 10.1074/jbc.m115.678573
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Lateral Diffusion, Function, and Expression of the Slow Channel Congenital Myasthenia Syndrome αC418W Nicotinic Receptor Mutation with Changes in Lipid Raft Components

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Cited by 6 publications
(3 citation statements)
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“…Similar discrepancies in diffusion coefficients calculated by FRAP or TIR FM have been reported previously for other plasma membrane proteins and may be explained by the fact that FRAP measurements can overestimate lateral diffusion by not considering that part of the fluorescence recovery is because of secretion (Ghosh et al, 2014). Regardless of this discrepancy, the diffusion coefficients obtained for fluorescently tagged PIN2 are within the range of what has been reported for other multispanning membrane proteins in animal and plant cells (Martinière et al, 2012;Oyola-Cintrón et al, 2015;Wang et al, 2016) and are useful estimates of the lateral diffusion of a potentially wide range of ESC RT cargo proteins.…”
Section: Simulation Of Esc Rt Cargo Diffusion During Ilv Formationsupporting
confidence: 84%
“…Similar discrepancies in diffusion coefficients calculated by FRAP or TIR FM have been reported previously for other plasma membrane proteins and may be explained by the fact that FRAP measurements can overestimate lateral diffusion by not considering that part of the fluorescence recovery is because of secretion (Ghosh et al, 2014). Regardless of this discrepancy, the diffusion coefficients obtained for fluorescently tagged PIN2 are within the range of what has been reported for other multispanning membrane proteins in animal and plant cells (Martinière et al, 2012;Oyola-Cintrón et al, 2015;Wang et al, 2016) and are useful estimates of the lateral diffusion of a potentially wide range of ESC RT cargo proteins.…”
Section: Simulation Of Esc Rt Cargo Diffusion During Ilv Formationsupporting
confidence: 84%
“…The diffusion coefficient D was calculated using the equation described by Padilla-Morales [4] . Where γ is a constant with a value of 0.88 corresponds to circular beams described by Oyola and Axelrod [ 11 , 12 ]. R is the radius of ROI and t 1/2 is the half-time of fluorescence recovery.…”
Section: Experimental Design Materials and Methodsmentioning
confidence: 99%
“…FRAP has been widely applied for studies on membrane nano-domains, by measuring the diffusion coefficients and immobile fractions of nano-domain related proteins. This has been achieved by manipulating the cholesterol levels in the cellular plasma membrane using methyl-β-cyclodextrin (MBCD) and then observing the changes in the diffusion coefficients via FRAP [83]. An increase in diffusion coefficient would mean that there are fewer or smaller nano-domains to slow down molecules resulting in faster recovery, while a measured slower diffusion coefficient would indicate the opposite result.…”
Section: Fluorescence Recovery After Photobleachingmentioning
confidence: 99%