Lateral Diffusion of Acridine Orange at Liquid Hydrocarbon/Water Interfaces

Kovaleski, John M.; Wirth, Mary J.

doi:10.1021/j100012a033

Cited by 48 publications

(58 citation statements)

References 5 publications

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“…[9][10][11] Lateral diffusion is measured very accurately by either fluorescence recovery after photobleaching [4][5][6][7][8][12][13][14] or fluorescence correlation spectroscopy, [15][16][17][18] where the latter avoids perturbing the sample. The interpretation of the results is most straightforward when there is a single diffusing species; however, the data can, in principle, be analyzed for multiple species.…”

Section: Introductionmentioning

confidence: 99%

Single-Molecule Study of an Adsorbed Oligonucleotide Undergoing Both Lateral Diffusion and Strong Adsorption

Wirth

Swinton

2001

J. Phys. Chem. B

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Single-molecule fluorescence spectroscopy was used to probe the lateral transport of an adsorbed oligonucleotide, the SP6 promoter primer, which is a 24-mer labeled at the 5′ end with tetramethylrhodamine. The oligonucleotide was adsorbed to the interface of an aqueous solution of 0.01 M KCl and silica chemically modified by chlorodimethyloctadecylsilane. Confocal fluorescence microscopy achieved single-molecule resolution, with a molecule in the beam 7% of the time. Autocorrelation of the data fit well to a model having two species, one diffusing and the other one undergoing transient strong adsorption. A small number of bursts, 0.3%, had unusually long durations, accounting for the slow component in the autocorrelation. When these long bursts were excised from the data, the autocorrelation fit well to simple diffusion, with D ) 4 × 10 -6 cm 2 /s. Autocorrelation of the long bursts alone gave a rate constant for desorption of 3 s -1 . The strongly adsorbed molecules were found to comprise 10% of the total population of adsorbates. It is concluded that the lateral transport of the SP6 promoter primer is described by fast lateral diffusion interrupted by rare, reversible, strong adsorption to defect sites. During strong adsorption, the tetramethylrhodamine label undergoes hindered motion, suggesting it is not the adsorbing moiety.

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Section: Introductionmentioning

confidence: 99%

Single-Molecule Study of an Adsorbed Oligonucleotide Undergoing Both Lateral Diffusion and Strong Adsorption

Wirth

Swinton

2001

J. Phys. Chem. B

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“…Similar to l 1 , l9 2 is equal to a well known chromatographic parameter, i. e. variance (r 2 ) of elution peak. Equations (3) - (8) indicate that the band broadening originates from the contributions of the several mass…”

Section: Moment Equationsmentioning

confidence: 99%

Moment analysis of chromatographic behavior in reversed‐phase liquid chromatography

Miyabe

2009

J of Separation Science

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Chromatographic behavior, such as sample retention, band broadening, elution peak profile, column efficiency, separation performance, and so on, depends on both the retention equilibrium and mass transfer kinetics in the column. A great number of research works have been carried out so far on the retention equilibrium in chromatography. However, chromatographic behavior has not so abundantly been investigated from kinetic points of view because of some essential difficulties of kinetic study. Additionally, in contrast with the extensive applications of various HPLC instruments and separation media developed in recent decades, it seems that arrangements of theoretical bases and experimental strategies relating to the kinetic study are not well recognized by most chromatographers. In this review, some items of information about the moment analysis method and concrete examples about mass transfer kinetics derived by the method are provided. The progress of some strategies for the kinetic study on chromatography is also introduced.

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“…Fluidity is a prominent feature of the phospholipid part of biological membranes, as well as of model phospholipid bilayer vesicle and monolayer systems, and it has been measured by different methods (Stoeckenius and Engelman, 1969;Kovaleski and Wirth, 1995). This process is present in the membrane fluidity characteristic, which in turn determines the function and stability of the membrane.…”

Section: Lateral Diffusion In Lipid Monolayersmentioning

confidence: 99%

Self-Assembly Monolayer Structures of Lipids and Macromolecules at Interfaces

Birdi¹

2002

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PREFACEThe information that science can extract from molecules that are essential for the processes of life can lead to better understanding of those processes. In natural selection during evolution (both prebiotic and biotic), physicochemical forces have been featured. A specific class of molecules, the amphiphiles, in aqueous environments exhibit self-assembly properties at liquid and solid interfaces. Such properties when combined with other forces require a special analysis. Of the self-assembly properties of amphiphiles, one is the formation of monomolecular films at both liquid-air and solid-air interfaces. During the last few decades, much has been reported in the literature on the formation of insoluble self-assembly monolayer (SAM) films of lipids and macromolecules (synthetic polymers and biopolymers) on the surface of water or at oil-water interfaces.The phenomenon of monolayer self-assembly has attracted considerable attention as a means of controlling molecular structures at interfaces. Studies of monomolecular films have been found to provide much physicochemical information on a molecular scale, which is useful for understanding many industrial and biological phenomena. For instance, the information obtained from lipid monolayer studies has been useful in determining the forces that stabilize various SAM structures found in lipid bilayers, vesicles, biological cell membranes, virus-cell fusion, emulsions, foams and films, and other self-assemblies. Current texts generally devote a single chapter to the characteristics of spread monolayers on liquids, and a researcher may have to look up a few hundred references to determine the procedures needed to investigate or analyze a particular phenomenon. Furthermore, there is an urgent need at this time for a text that discusses the state of the art regarding the surface phenomena exhibited by lipids and biopolymers, as they are relevant to a wide variety of surface and interfacial processes. During the last decade, the study of SAMs on solid surfaces has been aided greatly by developments in scanning tunneling microscopy (STM) and atomic force microscopy (AFM).The purpose of this book is to bring the reader up to date with the most recent experimental and the theoretical developments in relation to SAMs at liquid and solid surfaces. It is my intention to lead the researcher through the vast literature in such a way that he or she will be able to pursue particular investigation with suitable guidance. Such investigations may be industrial (emulsions, foams, dispersions, enhanced oil recovery, nonlinear optics) or biological [lipid-phase transitions, vesicles (drug targeting); bilayers, membranes, ion transport through membranes, sensors] in nature. The treatment in different parts of the text is based on the fundamental concepts of classical thermodynamics of surfaces (interfaces), i.e., the Gibbs adsorption theory. A large amount of experimental data from the literature has been included and critically examined from a vii viii PREFACE theoretical viewpoint...

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Lateral Diffusion of Acridine Orange at Liquid Hydrocarbon/Water Interfaces

Cited by 48 publications

References 5 publications

Single-Molecule Study of an Adsorbed Oligonucleotide Undergoing Both Lateral Diffusion and Strong Adsorption

Single-Molecule Study of an Adsorbed Oligonucleotide Undergoing Both Lateral Diffusion and Strong Adsorption

Moment analysis of chromatographic behavior in reversed‐phase liquid chromatography

Self-Assembly Monolayer Structures of Lipids and Macromolecules at Interfaces

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