Lateral diffusion of lectin receptors in fibroblast membranes as a function of cell shape

Swaisgood, Mark; Schindler, Melvin

doi:10.1016/0014-4827(89)90078-5

Cited by 24 publications

(9 citation statements)

References 43 publications

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“…Cell alignment appears to involve changes in the cell's actin cytoskeleton. Fibroblasts that are not attached to a surface are spherical in shape with either no apparent stress fibers [20] or actin primarily located near cytoplasmic precursors of focal contacts [7,8]. Within hours of attaching to a culture surface, the cell flattens and spreads, and active filaments form longitudinal stress fibers [13].…”

Section: Discussionmentioning

confidence: 99%

Fibroblast orientation to stretch begins within three hours

Neidlinger‐Wilke

Grood

Claes

et al. 2002

Journal Orthopaedic Research

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Most connective tissue cells align in response to stretch. Previous studies have shown these responses occur within 12-14 h of initiation of stretch, but do not identify the time at which this orientation occurs, nor whether the orientation continues after cessation of stretch. To ascertain the earliest times at which fibroblast orientation occurs, we cultured primary human fibroblasts on deformable culture dishes and stretched (1 Hz, 8% uniaxial strain) them for up to 24 h. We photographed the cells at 0.5, [1][2][3][4][5][6]8, 10, 12, 14,16, and 24 h. Similarly cells were photographed at 1-3, or 4 h after cessation of stretch for stretch durations of 1, 2, and 3 h. Orientation of cells were ascertained by an interactive computer program. The fibroblasts began to orient by 2-3 h and orientation appeared nearly complete by 24 h. Cultures stretched for 2 or 3 h continued to exhibit greater degrees of orientation (compared to controls) for 2 or 3 h respectively after cessation of stretch. We conclude fibroblasts begin to orient within 3 h of initiation of stretch, and that they continue to orient for several hours after cessation of stretch.

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Section: Discussionmentioning

confidence: 99%

Fibroblast orientation to stretch begins within three hours

Neidlinger‐Wilke

Grood

Claes

et al. 2002

Journal Orthopaedic Research

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“…The participation of the actin cytoskeleton in the formation of nanoscale domains was first postulated when it was observed that the coefficient of diffusion of phospholipid probes were significantly lower in live cells (Lee et al, 1993; Swaisgood and Schindler, 1989), than those estimated in artificial membranes (Ladha et al, 1996; Sonnleitner et al, 1999). This difference was also observed for transmembrane protein markers and glycosylphosphatidylinositol-anchored protein markers (Kusumi et al, 2012).…”

Section: N-3 Pufa and Lipid Rafts In The Cd4+ T Cell Plasma Membranementioning

confidence: 99%

Omega-3 fatty acids, lipid rafts, and T cell signaling

Hou

McMurray

Chapkin

2016

European Journal of Pharmacology

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n-3 PUFA have been shown in many clinical studies to attenuate inflammatory responses. Although inflammatory responses are orchestrated by a wide spectrum of cells, CD4+ T cells play an important role in the etiology of many chronic inflammatory diseases such as inflammatory bowel disease and obesity. In light of recent concerns over the safety profiles of non-steroidal anti-inflammatory drugs (NSAIDs), alternatives such as bioactive nutraceuticals are becoming more attractive. In order for these agents to be accepted into mainstream medicine, however, the mechanisms by which nutraceuticals such as n-3 polyunsaturated fatty acids (PUFA) exert their anti-inflammatory effects must be fully elucidated. Lipid rafts are nanoscale, dynamic domains in the plasma membrane that are formed through favorable lipid-lipid (cholesterol, sphingolipids, and saturated fatty acids) and lipid-protein (membrane-actin cytoskeleton) interactions. These domains optimize the clustering of signaling proteins at the membrane to facilitate efficient cell signaling which is required for CD4+ T cell activation and differentiation. This review summarizes novel emerging data documenting the ability of n-3 PUFA to perturb membrane-cytoskeletal structure and function in CD4+ T cells. An understanding of these underlying mechanisms will provide a rationale for the use of n-3 PUFA in the treatment of chronic inflammation.

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“…When needed, the value for the Stokes radius was converted to an aqueous diffusion constant at 37°C by the Stakes-Einstein equation: D = k*Tf6*pi*n*RH where k is Boltzmann's constant, T is temperature in Kelvin, n is the viscosity of water (lo-' cm2/sec), and RH is the Stokes radius. Physical properties used in predicting the diffusion constants were taken from the following sources: calcium (Kushmerick and Podolsky, 1969;Nasi and Tillotson, 1985;Wang, 1954), IP, (Berridge and Irvine, 19841, CAMP and cGMP (Tremblay et al, 19881, calmodulin (Klee and Vanaman, 19821, PKA (Carlson et al, 1979), cam kinase (Narin and Greengard, 1987), calcineuron (Klee et al, 1988;Nestler and Greengard, 1984), calpain (Fukui et al, 19881, cGMPdependent kinase (Gill and McCune, 19791, guanylate cyclase (Strada et al, 1984;Tremblay et al, 19881, CAMP phosphodiesterase (Beavo, 1988;, cam kinase I1 (Colbran and Soderling, 1990), DAG (Nandi and Wahl, 1988;Sassaroli et al, 1990;Swaisgood and Schindler, 1989;Wu et al, 1982), G-protein (alpha; Bierer et al, 1987;Dolphin, 1987;Ho et al, 1989;Stryer and Bourne, 1986), PKC (Dianoux et al, 1989;Kikkawa et al, 1989;Nishizuka, 1988;.…”

Section: Diffusion Propertiesmentioning

confidence: 99%

Local communication within dendritic spines: Models of second messenger diffusion in granule cell spines of the mammalian olfactory bulb

Woolf

Greer

1994

Synapse

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Dendritic spines are generally believed to play a role in modulating synaptically induced electrical events. In addition, they may also confine second messengers and thus topologically limit the distance over which second messenger cascades may be functionally significant. In order to address this possibility, computer simulations of transient second messenger concentration changes were performed. The results show the importance of spine morphology and binding and extrusion mechanisms in controlling second messenger transients. In the presence of intrinsic cytoplasmic binding sites and kinetic rates similar to that expected for calcium, second messengers were confined to the spine head. In the absence of binding/extrusion mechanisms, the size and time course of the input transient to the spine head influenced the second messenger transients that might be seen at the base of the spine neck and in other spines. Large and/or sustained increases in second messenger concentration in the spine head were communicated to the spine base and to other spine heads. The results emphasize the importance of a knowledge of breakdown pathways, concentrations and kinetics of binding sites, and extrusion mechanisms for understanding the dynamics of local chemical changes for dendritic spine function.

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Lateral diffusion of lectin receptors in fibroblast membranes as a function of cell shape

Cited by 24 publications

References 43 publications

Fibroblast orientation to stretch begins within three hours

Fibroblast orientation to stretch begins within three hours

Omega-3 fatty acids, lipid rafts, and T cell signaling

Local communication within dendritic spines: Models of second messenger diffusion in granule cell spines of the mammalian olfactory bulb

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