Lateral flow based immunobiosensors for detection of food contaminants

Raeisossadati, Mohammadjavad; Danesh, Noor Mohammad; Borna, Fazlollah; Gholamzad, Mehrdad; Ramezani, Mohammad; Abnous, Khalil; Taghdisi, Seyed Mohammad

doi:10.1016/j.bios.2016.06.061

Cited by 155 publications

(64 citation statements)

References 116 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For analysis of PHC and HPC (in triplicate) the normalization of the (L)LAB values was carried out by dividing the L values of the test lines by the L values of the control lines. The method of dividing the test line response by the control line response (T/C ratio) is a technique commonly used for the quantification of sandwich LFIAs [35,[47][48][49]. The results for PHC can be found in Figure 4, and the HPC smartphone calibration curve can be found in Figure S4 in Supplementary Information.…”

Section: Smartphone Readout and Analysismentioning

confidence: 99%

A Critical Comparison between Flow-through and Lateral Flow Immunoassay Formats for Visual and Smartphone-Based Multiplex Allergen Detection

2019

View full text Add to dashboard Cite

(1) Background: The lack of globally standardized allergen labeling legislation necessitates consumer-focused multiplexed testing devices. These should be easy to operate, fast, sensitive and robust. (2) Methods: Herein, we describe the development of three different formats for multiplexed food allergen detection, namely active and passive flow-through assays, and lateral flow immunoassays with different test line configurations. (3) Results: The fastest assay time was 1 min, whereas even the slowest assay was within 10 min. With the passive flow approach, the limits of detection (LOD) of 0.1 and 0.5 ppm for total hazelnut protein (THP) and total peanut protein (TPP) in spiked buffer were reached, or 1 and 5 ppm of THP and TPP spiked into matrix. In comparison, the active flow approach reached LODs of 0.05 ppm for both analytes in buffer and 0.5 and 1 ppm of THP and TPP spiked into matrix. The optimized LFIA configuration reached LODs of 0.1 and 0.5 ppm of THP and TPP spiked into buffer or 0.5 ppm for both analytes spiked into matrix. The optimized LFIA was validated by testing in 20 different blank and spiked matrices. Using device-independent color space for smartphone analysis, two different smartphone models were used for the analysis of optimized assays.

show abstract

Section: Smartphone Readout and Analysismentioning

confidence: 99%

A Critical Comparison between Flow-through and Lateral Flow Immunoassay Formats for Visual and Smartphone-Based Multiplex Allergen Detection

2019

View full text Add to dashboard Cite

show abstract

“…For the fabrication of disposable electrochemical and colorimetric sensors for food analysis, cellulose and its derivatives, such as nitrocellulose, are among the materials most commonly used . Cellulose‐based materials have the obvious advantage that they can be ultralow cost, and various biomolecules can be immobilized or freeze‐dried on the hydrophilic surface without much effort.…”

Section: Fields Of Applicationmentioning

confidence: 99%

Disposable Sensors in Diagnostics, Food, and Environmental Monitoring

et al. 2019

View full text Add to dashboard Cite

Disposable sensors are low‐cost and easy‐to‐use sensing devices intended for short‐term or rapid single‐point measurements. The growing demand for fast, accessible, and reliable information in a vastly connected world makes disposable sensors increasingly important. The areas of application for such devices are numerous, ranging from pharmaceutical, agricultural, environmental, forensic, and food sciences to wearables and clinical diagnostics, especially in resource‐limited settings. The capabilities of disposable sensors can extend beyond measuring traditional physical quantities (for example, temperature or pressure); they can provide critical chemical and biological information (chemo‐ and biosensors) that can be digitized and made available to users and centralized/decentralized facilities for data storage, remotely. These features could pave the way for new classes of low‐cost systems for health, food, and environmental monitoring that can democratize sensing across the globe. Here, a brief insight into the materials and basics of sensors (methods of transduction, molecular recognition, and amplification) is provided followed by a comprehensive and critical overview of the disposable sensors currently used for medical diagnostics, food, and environmental analysis. Finally, views on how the field of disposable sensing devices will continue its evolution are discussed, including the future trends, challenges, and opportunities.

show abstract

“…Some assays achieve high specificity by focusing on antibody-antigen interactions that form very rapidly. For example, in lateral flow devices each protein may have to be bound in seconds as it passes an immobilized capture antibody, thereby discriminating against nonspecific interaction with a slower on-rate [26,27]. Conversely, many assays require antibodies to remain bound to their target molecules for an extended time, again minimizing background from low-affinity interactions.…”

Section: What Affinity Reagents To Use?mentioning

confidence: 99%

A myopic perspective on the future of protein diagnostics

Landegren

Al-Amin

Björkesten

2017

Preprint

View full text Add to dashboard Cite

Plasma proteome analyses of the future promise invaluable insights into states of health, not only by measuring proteins whose role it is to ensure blood homeostasis, but increasingly also as a window into the health of practically any tissue in the body via so-called leakage protein biomarkers. Realizing more of this vast potential will require progress along many lines. Here we discuss the main ones, such as optimal selection of target proteins, affinity reagents, immunoassay formats, samples, and applications, with a view from ongoing work in our laboratory.

show abstract

Lateral flow based immunobiosensors for detection of food contaminants

Cited by 155 publications

References 116 publications

A Critical Comparison between Flow-through and Lateral Flow Immunoassay Formats for Visual and Smartphone-Based Multiplex Allergen Detection

A Critical Comparison between Flow-through and Lateral Flow Immunoassay Formats for Visual and Smartphone-Based Multiplex Allergen Detection

Disposable Sensors in Diagnostics, Food, and Environmental Monitoring

A myopic perspective on the future of protein diagnostics

Contact Info

Product

Resources

About