Lateral flow biosensors based on the use of micro- and nanomaterials: a review on recent developments

Huang, Yan; Xu, Tailin; Wang, Wenqian; Wen, Yongqiang; Li, Kun; Qian, Lisheng; Zhang, Xueji; Liu, Guodong

doi:10.1007/s00604-019-3822-x

Cited by 98 publications

(61 citation statements)

References 185 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even though a large number of graphene-based sensors reported in the literature exhibited good stability and repeatability, performance of some sensors in actual biological samples (such as blood and urine) often failed to achieve the desired detection results, which was mainly due to some non-specificity of biological and chemical molecules during the interfacial reaction of graphene and targets. Although some sample preparations (e.g., separation or preconcentration) are necessary before the final tests for biological samples, however, they always face the limits of complex procedures and are a time-consuming operation [76]. One effective solution to such problems is to develop ultrasensitive and high-specific sensors.…”

Section: Conclusion and Outlooksmentioning

confidence: 99%

Graphene-Based Biosensors for Detection of Biomarkers

2020

Self Cite

View full text Add to dashboard Cite

The development of biosensors with high sensitivity and low-detection limits provides a new direction for medical and personal care. Graphene and graphene derivatives have been used to prepare various types of biosensors due to their excellent sensing performance (e.g., high specific surface area, extraordinary electronic properties, electron transport capabilities and ultrahigh flexibility). This perspective review focuses on graphene-based biosensors for quantitative detection of cancer-related biomarkers such as DNA, miRNA, small molecules and proteins by integrating with different signal outputting approaches including fluorescent, electrochemistry, surface plasmon resonance, surface enhanced Raman scattering, etc. The article also discussed their challenges and potential solutions along with future prospects.

show abstract

Section: Conclusion and Outlooksmentioning

confidence: 99%

Graphene-Based Biosensors for Detection of Biomarkers

2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The sample-to-answer time for detecting human prostate specific antigen in a drop of blood (20 μL) is 15 min exhibiting a LOD of 0.028 ng/mL (21.4-fold improvement compared to that of conventional LFIA) demonstrating impressively the potential for sensitivity improvement by technological advancements [ 71 ]. The application of innovative reporter agents, e.g., nanozymes [ 72 ], up-converting phosphor technology-based lateral flow assay [ 73 ], antibody-gated indicator delivery systems [ 74 ], surface-enhanced Raman scattering (SERS) based LFIAs [ 75 ] as well as the combination with smartphone based readout [ 76 , 77 ] are other trends for LFIAs [ 78 ]. Tang et al [ 79 ] applied Eu/Tb (III) nanospheres with enhanced fluorescence as labels for anti-idiotypic nanobodies and monoclonal antibodies.…”

Section: Portable Immunoassay Techniques With Multiplexing Capabilmentioning

confidence: 99%

Multiplex Immunoassay Techniques for On-Site Detection of Security Sensitive Toxins

Pöhlmann

Elßner

2020

Toxins

View full text Add to dashboard Cite

Biological toxins are a heterogeneous group of high molecular as well as low molecular weight toxins produced by living organisms. Due to their physical and logistical properties, biological toxins are very attractive to terrorists for use in acts of bioterrorism. Therefore, among the group of biological toxins, several are categorized as security relevant, e.g., botulinum neurotoxins, staphylococcal enterotoxins, abrin, ricin or saxitoxin. Additionally, several security sensitive toxins also play a major role in natural food poisoning outbreaks. For a prompt response to a potential bioterrorist attack using biological toxins, first responders need reliable, easy-to-use and highly sensitive methodologies for on-site detection of the causative agent. Therefore, the aim of this review is to present on-site immunoassay platforms for multiplex detection of biological toxins. Furthermore, we introduce several commercially available detection technologies specialized for mobile or on-site identification of security sensitive toxins.

show abstract

“…Lateral flow-based techniques have been developed for rapid detection of various infectious and analytical agents during the past few years [96] that meets the affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverable to end-users (ASSURED) criteria of the world health organization (WHO) [97]. A lot of lateral flow assays with different accuracy, specificity, and sensitivity were developed.…”

Section: Lateral Flow-based Techniquesmentioning

confidence: 99%