Lateral flow dipstick antigen assay for human cystic echinococcosis

Khanbabaie, Sam; Riazi, Mehdi; Chang, Chiat Han; Yunus, Muhammad Hafiznur; Noordin, Rahmah

doi:10.1016/j.actatropica.2018.11.018

Cited by 15 publications

(19 citation statements)

References 40 publications

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“…All above mentioned clinical and history data didn't intercede for accurate diagnosis of hydatidosis so serological tests improvement is still needed especially detecting the circulating Echinococcus antigen in cystic echinococcosis patients [34] and introducing new materials like nanogold particles for functionalization of detecting antibodies used in ELISA techniques enhanced the sensitivity and reduced the analysis time of these techniques [35].…”

Section: Discussionmentioning

confidence: 99%

“…We used in this study nanogold particles to load the detecting ppAb in sandwich ELISA technique for the first time in hydatidosis serodiagnosis to detect diagnostic circulating antigens, lateral flow dipstick assay was used by [34] to prepare three hydatid antigens including hydatid cyst fluid, native antigen B and recombinant antigen B to raise polyclonal rabbit antiserum against each antigen and the IgG fractions were conjugated on gold nanoparticles to detect those antigens. The dotimmunogold staining method was used to detect antibodies against hydatid cyst disease in sheep by preparing antigen B which was labeled with gold nanoparticles and analyzed by SDS-PAGE thus nanoparticles produced a typical purple color by binding to the strip at the site of immunoreaction [36].…”

Section: Discussionmentioning

confidence: 99%

“…Hydatidosis (cystic echinococcosis) has worldwide distribution representing a major public health problem especially those concerning sheep and cows grazing, so its diagnosis represented an important issue and continuous improvement is needed. Hydatidosis diagnosis is dependent on clinical presentation and serodiagnosis so the development of the serodiagnostic technique is a necessary request [1]. Monitoring of hydatid fluid to react against IgG antibodies was a guide for hydatidosis diagnosis but on following up, it was shown to be useless as the anti-hydatid fluid antibodies persist for long time in patients' sera in addition to the appearance of false positive and negative results [2].…”

Section: Introductionmentioning

confidence: 99%

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A pioneer usage of nanogold sandwich ELISA in the detection of hydatid antigen in humans as a tool for improvement of serodiagnostic testing.

Elawamy

Rashed

Nassr

et al. 2020

Preprint

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Background: Human cystic echinococcosis is a zoonosis occurring due to dogs handling and exposure to Echinococcus granulosus ova in their stools being an accidental intermediate host. Serology remains the only sure and important tool to diagnose this disease. Our objectives in this research are to improve the serodiagnostic abilities of sandwich ELISA through its binding to gold nanoparticle and proving the role of minute nanoparticles in serodiagnosis. Methods: gold nanoparticles (AuNPs) were employed for the capture of the antigens of protoscolices (pAg) in the patient’s serum by sandwich ELISA. Cross-reactivity for antigens from Hymenolepis nana , Entrobius vermicularis , and Fasciola gigantica was ruled out by using anti-protoscolices polyclonal antibodies (ppAb). (pAg) of sonicated protoscolices which were removed from camel lung cysts, was purified by diethyl aminoethyl Sephadex (DEAE-Sephadex) and injected to a New zealand white rabbit giving ppAb which then conjugated to horseradish peroxidase (HRP) and loaded on AuNPs being used as a diagnostic indicator for circulating pAg by both sandwich ELISA and nanogold sandwich ELISA techniques. Results: Nanogold sandwich ELISA was able to give positive results with 96.3% of hydatid patients and 5% of non-hydatid patients while sandwich ELISA showed 81.4% and 20% positive cases of the same groups respectively. The sensitivity and specificity of nanogold sandwich ELISA were 96.3% & 95% against 81.5% & 80% for those of sandwich ELISA respectively. Conclusion: The conjugation of AuNPs to anti- Echinococcus IgG antibodies seems to be beneficial to maximize the sensitivity of sandwich ELISA, reduce the overall cost of the assay as less antibody was needed, solve harsh buffering conditions and achieve greater stability which provides a reliable quantitation of analytes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A pioneer usage of nanogold sandwich ELISA in the detection of hydatid antigen in humans as a tool for improvement of serodiagnostic testing.

Elawamy

Rashed

Nassr

et al. 2020

Preprint

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“…Potassium ferrocyanide (K 4 Fe(CN) 6 ), bovine serum albumin (BSA), potassium ferricyanide (K 3 Fe(CN) 6 ), ethanol amine, cysteamine hydrochloride, sulphuric acid, disodium hydrogen orthophosphate, 1,4‐phenylene diisothiocyanate (PDITC), potassium dihydrogen orthophosphate, pyridine, N,N‐dimethyl formamide (DMF) and sodium chloride were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Recombinant AgB and purified rabbit polyclonal antibody against recombinant AgB were produced at INFORMM as described previously . Rabbit polyclonal anti‐dystrophin antibodies were purchased from Abcam (MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis

2020

Self Cite

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Cystic echinococcosis (CE) or hydatid disease is a parasitic infection caused by Echinococcus granulosus. Early serodiagnosis and continuous monitoring of the disease is very important for medical treatment. Here, we report the detecting of both echinococcus antigen and antibody for the diagnosis of hydatid disease using square wave voltammetry (SWV)‐based immunosensors. The gold electrodes were functionalized using cysteamine/phenylene diisothiocyanate linkers and used for the immunosensors fabrication. The hydatid antigen and antibody immunosensors were constructed by the immobilization of either purified rabbit polyclonal antibody or recombinant antigen B (AgB), respectively on the functionalized gold electrodes surfaces. The detection in both cases was achieved by following the change in the SWV reduction peak current of the ferro/ferricyanide redox couple upon antibody or antigen binding. These immunosensors enabled the detection of echinococcus antigen and antibody within a concentration range of 1 pg.mL−1 to 1 μg.mL−1 with detection limits of 0.4 pg.mL−1 and 0.3 pg.mL−1, respectively. A preliminary application of the developed immunosensor was performed in spiked serum sample showing good recovery percentages ranging from 102 to 110 % for both hydatid antibody and antigen detection. This easy‐to‐use, sensitive, and low cost quantitative method holds great promise for the early diagnosis of hydatid disease and thus, better managements and treatment outcomes.

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“…The disease has a wide geographical scope and occurs in all continents, including the tropical and subtropical regions. Cystic echinococcosis (hydatid cyst) (CE) was diagnosed by the basic methods of clinical examination, imaging and serological tests (5)(6)(7). CE is treated by a variety of ways, including chemical therapy (drug therapy), percutaneous aspiration injection and respiration (PAIR), surgical treatment, or the option of observation without surgical intervention (8,9).…”

Section: Introductionmentioning

confidence: 99%

Application of ultrasound contra infection with secondary hydatidosis in mice

Ramdan¹,

Ali²

2020

IJVS

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The present research explained the impact of ultrasound on the viability of Echinococcus granulosus protoscolices in vitro application of fixed frequency waves at 20000 pulse/sec and 1.8 w/cm 2 , with different exposing periods, thirty, twenty, fifteen, twelve, ten and five seconds respectively, subsequently prevention of mice against interjected with minor hydatid cysts, in comparison with the control animals infected with protoscolices without exposure to ultrasound waves, depending on many criteria including the numbers, weights and diameters of developed hydatid cysts. The results displayed an obvious impact of ultrasound waves on Echinococcus granulosus protoscolices viability number by increase of exposure period in vitro, in addition to a decrease of 100% of hydatid cysts numbers in the dealt ones with 17% fertility exposed for 20 seconds, the reduction rate was 99.23% in the group of fertility 35% which exposed for 15 seconds, after four and five months of infection.

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Lateral flow dipstick antigen assay for human cystic echinococcosis

Cited by 15 publications

References 40 publications

A pioneer usage of nanogold sandwich ELISA in the detection of hydatid antigen in humans as a tool for improvement of serodiagnostic testing.

A pioneer usage of nanogold sandwich ELISA in the detection of hydatid antigen in humans as a tool for improvement of serodiagnostic testing.

Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis

Application of ultrasound contra infection with secondary hydatidosis in mice

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