Lateral flow immunochromatographic assay for rapid screening of faecal carriage of carbapenemase-producing Enterobacteriaceae

Fauconnier, Charlotte; Dodémont, Magali; Depouhon, Angélique; Anantharajah, Ahalieyah; Verroken, Alexia; Rodríguez-Villalobos, Hector

doi:10.1093/jac/dky429

Cited by 12 publications

(12 citation statements)

References 9 publications

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“…The fact that the test can quickly identify the intestinal carriage of different types of carbapenemases belonging to the five most common carbapenemase families is also important when choosing empirical therapy for severe infections or immunocompromised patients. Our results are comparable to those by Fauconnier et al who tested the immunochromatographic assay OKN K-SeT test for rapid screening of CPO fecal carriage and reported 100% specificity and 96% sensitivity [ 14 ].…”

Section: Discussionsupporting

confidence: 88%

“…The supernatant was discarded, and the pellet was resuspended with the lysis buffer provided in the NG-Test CARBA 5 kit (four drops, 120 μL) and processed following the manufacturer’s instructions. The presence of carbapenemases was confirmed by multiplex PCR [ 14 ] detecting bla OXA-48 , bla KPC , bla NDM , bla IMP , and bla VIM . DNA extraction was performed from the broth (100 μL) after incubating for two hours (PureLink Genomic DNA Kits, Invitrogen, Life technologies, Carlsbad, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

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Detection of KPC, NDM and VIM-Producing Organisms Directly from Rectal Swabs by a Multiplex Lateral Flow Immunoassay

et al. 2021

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We report a preliminary evaluation of the NG-Test CARBA 5 immunochromatographic assay for detecting carbapenemases directly from rectal swabs on the same day of sampling. Thirty fecal swabs were examined for carbapenemase-producing organisms (CPOs) by conventional culture, PCR, and NG-Test CARBA 5. Each sample was tested by the immunochromatographic assay five times, including direct testing and incubation in trypticase soy broth for 1, 2, 3, and 4 h. Twenty patients yielded CPOs by culture. Immunochromatographic and PCR results were concordant and detected the same 25 carbapenemases (11 KPC, 8 VIM, and 6 NDM). In five cases, we detected co-carriage of KPC and VIM. Compared with PCR, the sensitivity of NG-Test CARBA 5 for the detection of KPC, VIM, and NDM was 80% without incubation, 88% with one hour, 92% with two, and 100% with three hours incubation, while specificity was 100% for all time points. All samples containing adequate fecal content were detected by NG-Test CARBA 5 concordantly with PCR, without incubation. NG-Test CARBA 5 is a reliable test that rapidly detects the presence of carbapenemases at the same day of sampling, directly from rectal swabs. It thus provides early information to guide antimicrobial treatment and infection control interventions.

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Section: Discussionsupporting

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Detection of KPC, NDM and VIM-Producing Organisms Directly from Rectal Swabs by a Multiplex Lateral Flow Immunoassay

et al. 2021

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“…The RDT used in this study was designed to be performed on culture-grown colonies on agar plates. However, previous studies employed different versions of this RDT as a screening method for faecal carriage of multiresistant bacteria directly on rectal swabs [4], with comparable diagnostic accuracy if compared with culture [14]. With regard to positive blood culture bottles, a proof-ofprinciple study conducted on the previous version of this RDT (RESIST-3) showed a sensitivity and specificity of 100%, respectively, for carbapenemase detection if blood cultures were spiked in the laboratory with carbapenemase-producing bacteria and the test applied on a centrifuged lysate of positive blood cultures [15].…”

Section: Discussionmentioning

confidence: 99%

Application and clinical impact of the RESIST-4 O.K.N.V. rapid diagnostic test for carbapenemase detection in blood cultures and clinical samples

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et al. 2020

Eur J Clin Microbiol Infect Dis

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Invasive infections caused by carbapenemase-producing bacteria are associated with excess mortality. We applied a rapid diagnostic test (RDT) on clinical samples with an elevated likelihood of carbapenemase-producing bacteria and documented its impact on antibiotic treatment decisions. Among 38 patients, twelve tested positive for infections caused by carbapenemase-producing bacteria (31.6%), mainly in blood cultures. KPC (n = 10) was more frequent than OXA-48 (n = 2). RDT-based carbapenemase detection led to a treatment modification to ceftazidime/avibactam-containing regimens in all patients before detailed antibiotic testing results became available. Eleven patients (92%) survived the acute infection, whereas one patient with a ceftazidime/avibactam- and colistin-resistant OXA-48-positive isolate died.

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“…Other teams have used centrifugation and washing steps for the detection of carbapenemases from rectal swabs using LFIA. However, for this sample matrix, prior incubation in the presence of an antibiotic is necessary [ 116 , 117 ].…”

Section: Direct Detection Of Antibiotic-resistant Bacteria In Clinica...mentioning

confidence: 99%

The Revolution of Lateral Flow Assay in the Field of AMR Detection

et al. 2022

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The global spread of antimicrobial resistant (AMR) bacteria represents a considerable public health concern, yet their detection and identification of their resistance mechanisms remain challenging. Optimal diagnostic tests should provide rapid results at low cost to enable implementation in any microbiology laboratory. Lateral flow assays (LFA) meet these requirements and have become essential tools to combat AMR. This review presents the versatility of LFA developed for the AMR detection field, with particular attention to those directly triggering β-lactamases, their performances, and specific limitations. It considers how LFA can be modified by detecting not only the enzyme, but also its β-lactamase activity for a broader clinical sensitivity. Moreover, although LFA allow a short time-to-result, they are generally only implemented after fastidious and time-consuming techniques. We present a sample processing device that shortens and simplifies the handling of clinical samples before the use of LFA. Finally, the capacity of LFA to detect amplified genetic determinants of AMR by isothermal PCR will be discussed. LFA are inexpensive, rapid, and efficient tools that are easy to implement in the routine workflow of laboratories as new first-line tests against AMR with bacterial colonies, and in the near future directly with biological media.

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Lateral flow immunochromatographic assay for rapid screening of faecal carriage of carbapenemase-producing Enterobacteriaceae

Cited by 12 publications

References 9 publications

Detection of KPC, NDM and VIM-Producing Organisms Directly from Rectal Swabs by a Multiplex Lateral Flow Immunoassay

Detection of KPC, NDM and VIM-Producing Organisms Directly from Rectal Swabs by a Multiplex Lateral Flow Immunoassay

Application and clinical impact of the RESIST-4 O.K.N.V. rapid diagnostic test for carbapenemase detection in blood cultures and clinical samples

The Revolution of Lateral Flow Assay in the Field of AMR Detection

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