Lateral Flow Strip Biosensors for Foodborne Pathogenic Bacteria via Direct and Indirect Sensing Strategies: A Review

Abstract

Rapid and sensitive detection of foodborne pathogenic bacteria is particularly important for the prevention and control of foodborne diseases. The lateral flow strip biosensor (LFSB) is one of the most promising point-of-care detection tools and has been widely used in food safety monitoring. This review introduces recent advances in the detection of foodborne pathogenic bacteria using LFSBs. According to different bacterial biomarkers, we summarize the direct and indirect sensing strategies of bacterial LFSBs. The direct sensing strategies for whole bacterial cells are divided into antibodies, antibody alternatives, and label-free according to the recognition elements. The indirect sensing strategies refer to the detection of bacterial nucleic acids and metabolites. Next, we compare and discuss the applications of direct and indirect sensing strategies. Finally, the existing challenges, future perspectives, and development directions are discussed, which will facilitate the theoretical innovation and practical application for bacterial LFSBs.

Electrochemical Evaluation of the Number of Viable Bacteria Using Carbon Electrode Chip

“…1,2 The ingestion of food and drinking water contaminated with these pathogens causes various symptoms, such as diarrhea, abdominal pain, vomiting, and, in severe cases, hemolytic uremic disease. 3 According to a World Health Organization (WHO) and World Bank study, 600 million cases of foodborne illness occur worldwide annually, costing lowand middle-income economies approximately US $110 billion in lost productivity and medical costs. Therefore, rapid detection of pathogenic bacteria is necessary to prevent health hazards such as food poisoning and infectious diseases, as well as reduce economic losses such as medical expenses.…”

Salivary endotoxin detection using combined mono/polyclonal antibody-based sandwich-type lateral flow immunoassay device

“…Since LPS is a large biomacromolecule with multiple binding sites, one can beneficially utilize the more sensitive sandwich-type immunoassay rather than the competitive approach. 29 The size of LPS aggregates in our buffer solutions were evaluated using dynamic light scattering (DLS) method (Fig. S1†).…”

Simultaneous Electrochemical Detection of Multiple Bacterial Species Using Metal–Organic Nanohybrids

“…1,2 The ingestion of food and drinking water contaminated with these pathogens causes various symptoms such as diarrhea, abdominal pain, and vomiting, and in severe cases, hemolytic uremic disease. 3 According to a study by the World Health Organization (WHO) and the World Bank, 600 million cases of foodborne illness occur worldwide annually, costing low-and middle-income economies approximately US $110 billion in lost productivity and medical costs. Therefore, the rapid detection of pathogenic bacteria is necessary to prevent health hazards such as food poisoning and infectious diseases and to reduce economic losses as medical expenses.…”

“…Pathogenic Escherichia coli, Salmonella enterica, and Staphylococcus aureus are well known as causative organisms of food poisoning. , The ingestion of food and drinking water contaminated with these pathogens causes various symptoms such as diarrhea, abdominal pain, and vomiting, and in severe cases, hemolytic uremic disease . According to a study by the World Health Organization (WHO) and the World Bank, 600 million cases of foodborne illness occur worldwide annually, costing low- and middle-income economies approximately US $110 billion in lost productivity and medical costs.…”