Lateral flow test for meat authentication with visual detection

Magiati, Maria; Myridaki, Vasiliki M.; Christopoulos, Theodore K.; Kalogianni, Despina P.

doi:10.1016/j.foodchem.2018.09.063

Cited by 69 publications

(26 citation statements)

References 14 publications

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“…Recently, several applications of RPA in DNA and RNA target detection have been proved [ 20 , 21 ], and the integration of RPA in different platforms has been reported [ 22 , 23 , 24 ]. Lateral flow dipsticks (LFD) has great application prospects in the detection field due to easy-to-use, high sensitivity, less time consuming and low-cost [ 25 , 26 , 27 ]. Furthermore, RPA products can be detected with lateral flow dipstick (LFD) which only requires 5 min to complete all test process without any special equipment, and the results can be observed by naked eyes [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Recombinase Polymerase Amplification Based Multiplex Lateral Flow Dipstick for Fast Identification of Duck Ingredient in Adulterated Beef

Zhang

Zhou

et al. 2020

Animals

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Meat adulteration has become a global social problem. In order to protect consumers from meat adulteration, several methods have been developed to identify meat species. However, the conventional methods are labor-intensive, time-consuming and require instruments. In the present study, a rapid and visual method based on recombinase polymerase amplification (RPA) and multiplex lateral flow dipstick (MLFD) was developed to detect duck ingredient in adulterated beef. Using recombinase and strand displacement polymerase enable RPA to amplify different double-labeled DNA amplicons at room temperature, which can be further detected by MLFD. The whole reaction process can be finished within 35 min, and the results can be determined by naked eyes. As low as 5% of duck ingredient in adulterated beef can be easily measured. Moreover, we confirmed that our new method held good potential in the detection of commercially processed meat samples. In conclusion, this study reported a useful animal derived meat adulteration detection method, which have potential application in future.

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Section: Introductionmentioning

confidence: 99%

Recombinase Polymerase Amplification Based Multiplex Lateral Flow Dipstick for Fast Identification of Duck Ingredient in Adulterated Beef

Zhang

Zhou

et al. 2020

Animals

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“…Colorimetric detection represents the most common detection method in paper-based assays for food safety monitoring mainly due to its simplicity and ability to be detected by the naked eyes [102]. In fact, a numbers of lateral flow test strips have been developed for the identification of food contaminations such as foodborne pathogens, chemicals and food adulterations [103]. Generally, in lateral flow assay, the amount of targets could be estimated based upon the gold nanoparticles (AuNPs)-induced color changes.…”

Section: Recent Advances Of Poc Diagnostic Techniques For Food Safmentioning

confidence: 99%

Emerging Point-of-care Technologies for Food Safety Analysis

Choi

Yong

Choi

et al. 2019

Sensors

132

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Food safety issues have recently attracted public concern. The deleterious effects of compromised food safety on health have rendered food safety analysis an approach of paramount importance. While conventional techniques such as high-performance liquid chromatography and mass spectrometry have traditionally been utilized for the detection of food contaminants, they are relatively expensive, time-consuming and labor intensive, impeding their use for point-of-care (POC) applications. In addition, accessibility of these tests is limited in developing countries where food-related illnesses are prevalent. There is, therefore, an urgent need to develop simple and robust diagnostic POC devices. POC devices, including paper- and chip-based devices, are typically rapid, cost-effective and user-friendly, offering a tremendous potential for rapid food safety analysis at POC settings. Herein, we discuss the most recent advances in the development of emerging POC devices for food safety analysis. We first provide an overview of common food safety issues and the existing techniques for detecting food contaminants such as foodborne pathogens, chemicals, allergens, and toxins. The importance of rapid food safety analysis along with the beneficial use of miniaturized POC devices are subsequently reviewed. Finally, the existing challenges and future perspectives of developing the miniaturized POC devices for food safety monitoring are briefly discussed.

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“…Streptavidin (SA) protein forms strong noncovalent bonds with biotin; hence, it is conjugated with AuNPs (for conjugation) or immobilized at T‐line (for recognition) (Zhao, Wang, et al., 2020). Some antibody‐independent NA‐LFA methods have also been developed in which biotinylated probes or amplicons have been used with SA or poly(dT) sequence as a binding molecule (Kortli et al., 2020; Magiati, Myridaki, Christopoulos, & Kalogianni, 2019; Ngom, Guo, Wang, & Bi, 2010). A number of NA‐LFAs or NA‐LFS or NA‐LFD devices have been developed for POC detection of animal species in different meat products (Figure 1) (Li et al., 2019; Yan et al., 2020; Zhang, Zhang, et al., 2019; Zhao, Wang, et al., 2020; Zheng et al., 2020).…”

Section: Poc Testing Devicesmentioning

confidence: 99%

“…It was also suggested that this approach can be modified and used for the identification of other meat species using modified primer sets. An LFA‐based visual detection method was developed for the identification of horse and pork species in their binary mixture with beef and sheep meat (Magiati et al., 2019). Biotinylated primers were used during amplification of DNA from different animal species.…”

Section: Recent Developments In Poc Devices For Meat Species Identifimentioning

confidence: 99%

Rapid point‐of‐care testing methods/devices for meat species identification: A review

Kumar

Narsaiah

2020

Comp Rev Food Sci Food Safe

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The authentication of animal species is an important issue due to an increasing trend of adulteration and mislabeling of animal species in processed meat products. Polymerase chain reaction is the most sensitive and specific technique for nucleic acid‐based animal species detection. However, it is a time‐consuming technique that requires costly thermocyclers and sophisticated labs. In recent times, there is a need of on‐site detection by point‐of‐care (POC) testing methods and devices under low‐resource settings. These POC devices must be affordable, sensitive, specific, user‐friendly, rapid and robust, equipment free, and delivered to the end users. POC devices should also confirm the concept of micro total analysis system. This review discusses POC testing methods and devices that have been developed for meat species identification. Recent developments in lateral flow assay‐based devices for the identification of animal species in meat products are also reviewed. Advancements in increasing the efficiency of lateral flow detection are also discussed.

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Lateral flow test for meat authentication with visual detection

Cited by 69 publications

References 14 publications

Recombinase Polymerase Amplification Based Multiplex Lateral Flow Dipstick for Fast Identification of Duck Ingredient in Adulterated Beef

Recombinase Polymerase Amplification Based Multiplex Lateral Flow Dipstick for Fast Identification of Duck Ingredient in Adulterated Beef

Emerging Point-of-care Technologies for Food Safety Analysis

Rapid point‐of‐care testing methods/devices for meat species identification: A review

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