Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed

Wang, Zhizeng; Zhi, Dejuan; Zhao, Yue; Zhang, Hailong; Wang, Xin; Ru, Yi; Li, Hongyu

doi:10.2147/ijn.s58942

Cited by 61 publications

(24 citation statements)

References 36 publications

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“…This is consistent with other colloidal gold LFIAs that have demonstrated reliability up to one year. 31 , 32 This stability allows for strips used at the point-of-care to undergo reevaluation at a centralized laboratory for quality assurance.…”

Section: Resultsmentioning

confidence: 99%

Noninvasive Urine Biomarker Lateral Flow Immunoassay for Monitoring Active Onchocerciasis

et al. 2018

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The parasitic disease onchocerciasis is the second leading cause of preventable blindness, afflicting more than 18 million people worldwide. Despite an available treatment, ivermectin, and control efforts by the World Health Organization, onchocerciasis remains a burden in many regions. With an estimated 120 million people living in areas at risk of infection, efforts are now shifting from prevention to surveillance and elimination. The lack of a robust, point-of-care diagnostic for an active Onchocerca infection has been a limiting factor in these efforts. Previously, we reported the discovery of the biomarker N-acetyl-tyramine-O-glucuronide (NATOG) in human urine samples and its ability to track treatment progression between medicated patients relative to placebo; we also established its capability to monitor disease burden in a jird model. NATOG is a human-produced metabolite of tyramine, which itself is produced as a nematode neurotransmitter. The ability of NATOG to distinguish between active and past infection overcomes the limitations of antibody biomarkers and PCR methodologies. Lateral flow immunoassay (LFIA) diagnostics offer the versatility and simplicity to be employed in the field and are inexpensive enough to be utilized in large-scale screening efforts. Herein, we report the development and assessment of a NATOG-based urine LFIA for onchocerciasis, which accurately identified 85% of analyzed patient samples (N = 27).

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Section: Resultsmentioning

confidence: 99%

Noninvasive Urine Biomarker Lateral Flow Immunoassay for Monitoring Active Onchocerciasis

et al. 2018

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“…86 Dinitrobutylphenol is very detrimental to human and aquatic animals as it has carcinogenicity. Dinitrobutylphenol is released in to waste water causing environmental pollution, SeNPs based chemiluminescence system is, so far, best for its detection.…”

Section: Iii) Sensormentioning

confidence: 99%

Selenium nanostructures: microbial synthesis and applications

Shirsat¹,

Kadam²,

Naushad

et al. 2015

RSC Adv.

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Due to considerable impact on day-to-day life through superior functioning of energy, medicine, electronics, sensors and space industries young researchers are being attracted towards nanotechnology/biotechnology field. Selenium (Se) is acting as an essential element in most of the organism and beneficial nutrient in higher plants. Se nanostructures (NSs) i.e. nanospheres or particles (NPs) and nanowires (NWs) have unusual properties compared to their bulk counterparts. It has seven-fold lower toxicity than its other biologically utilizable materials. Several physical & chemical methods are being applied for the synthesis of Se NSs, but development of eco-friendly routes is of considerable importance towards biological applications. Recently microorganisms are being reported for the synthesis of Se NSs by reduction of oxyions in anaerobic and aerobic environments. This review briefly proposes the recent developments in the microbial synthesis, characterization, synthesis mechanism of Se NSs along with non-biological (solar, sensor, photocatalysis etc.) and biological (anticancer, antioxidant, antiprotozoal etc.) applications. Advantages of Se NSs synthesized using green approaches, over commonly used, are explored.

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“…Selenium nanoparticles are prepared at room temperature, easily bind to proteins, and are not sensitive to electrolytes. They also have higher levels of sensitivity, and are more economical to prepare than other probe types [19]. The current study is based on our reported article on how to prepare selenium nanoparticles [18].…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Anti-SARS-CoV-2 IgM and IgG Using a Selenium Nanoparticle-based Lateral Flow Immunoassay

Wang

Zheng

Wang

et al. 2020

Preprint

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Background: Coronavirus disease 2019 is an infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-CoV-2 is highly transmissible. Early and rapid testing is necessary to effectively prevent and control the outbreak. Detection of SARS-CoV-2 antibodies with lateral flow immunoassay can achieve this goal. Antibody detection is especially effective for the detection of asymptomatic infection.Methods: In this study, SARS-CoV-2 nucleoprotein was expressed by E. coli and purified by affinity chromatography. We used the highly stable and sensitive selenium nanoparticle as the labeling probe coupled with the SARS-CoV-2 nucleoprotein to prepare a new SARS-CoV-2 antibody (IgM and IgG) detection kit. The sensitivity and specificity of the kit were verified by plasma of COVID-19 patients and health persons. Separate detection of IgM and IgG, such as in this assay, was performed in order to reduce mutual interference and improve the accuracy of the test results.Results: The SARS-CoV-2 nucleoprotein was purified on a nickel column, and the final purity was greater than 90%. The sensitivity of the kit was 94.74% and the specificity was 95.12% by 41 negative plasma samples and 19 positive plasma samples detection.Conclusions: The assay kit does not require any special device for reading the results and the readout is a simple color change that can be evaluated with the naked eye. This kit is suitable for rapid and real-time detection of the SARS-CoV-2 antibody.

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Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed

Cited by 61 publications

References 36 publications

Noninvasive Urine Biomarker Lateral Flow Immunoassay for Monitoring Active Onchocerciasis

Noninvasive Urine Biomarker Lateral Flow Immunoassay for Monitoring Active Onchocerciasis

Selenium nanostructures: microbial synthesis and applications

Rapid Detection of Anti-SARS-CoV-2 IgM and IgG Using a Selenium Nanoparticle-based Lateral Flow Immunoassay

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