Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice

Alder, Janet; Fujioka, Wendy; Lifshitz, Jonathan; Crockett, David P.; Thakker‐Varia, Smita

doi:10.3791/3063-v

Cited by 8 publications

(6 citation statements)

References 3 publications

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“…LFPI requires a craniectomy in the parietal bone, and the assembly of an injury hub onto the skull. Using the fluid percussion device, a fluid impulse is delivered directly to the brain through the injury hub, onto the intact dura, which causes a focal lesion to the brain, as well as applying rotational forces to the brain that results in widespread diffuse pathology ( Alder et al, 2011 ). The craniectomy site can be adjusted to change the location of the focal lesion (i.e., frontal bone; Ouyang et al, 2018 ).…”

Section: Pre-clinical Models Of Tbimentioning

confidence: 99%

Sleep, inflammation, and hemodynamics in rodent models of traumatic brain injury

Green,

Carey,

Mannino

et al. 2024

Front. Neurosci.

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Traumatic brain injury (TBI) can induce dysregulation of sleep. Sleep disturbances include hypersomnia and hyposomnia, sleep fragmentation, difficulty falling asleep, and altered electroencephalograms. TBI results in inflammation and altered hemodynamics, such as changes in blood brain barrier permeability and cerebral blood flow. Both inflammation and altered hemodynamics, which are known sleep regulators, contribute to sleep impairments post-TBI. TBIs are heterogenous in cause and biomechanics, which leads to different molecular and symptomatic outcomes. Animal models of TBI have been developed to model the heterogeneity of TBIs observed in the clinic. This review discusses the intricate relationship between sleep, inflammation, and hemodynamics in pre-clinical rodent models of TBI.

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Section: Pre-clinical Models Of Tbimentioning

confidence: 99%

Sleep, inflammation, and hemodynamics in rodent models of traumatic brain injury

Green,

Carey,

Mannino

et al. 2024

Front. Neurosci.

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show abstract

“…With this change and control, researchers can examine the consequences of mild TBI, which is a prevalent type of TBI in sports-related injuries. 35…”

Section: Cortical Impact Modelmentioning

confidence: 99%

“…This alteration enables researchers to investigate the impact of repetitive mild TBI, a prevalent kind of TBI in sports-related injuries. 35 When compared to the CCI model, the FPI results in a more widespread injury and primarily causes moderate to severe TBI. To research diffuse injuries, the FPI would therefore be more appropriate.…”

Section: Controlled Cortical Impact Model Fluid Percussion Modelmentioning

confidence: 99%

Experimental animal models in traumatic brain injury research: a comprehensive review of methods and outlook

Saidu,

Bulama,

Onu

et al. 2024

Int J Sci Rep

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Traumatic brain injury (TBI) remains a significant public health concern worldwide, necessitating effective research models to elucidate its pathophysiology and develop therapeutic interventions. Animal models play a crucial role in TBI research, offering valuable insights into injury mechanisms and potential treatments. However, selecting the appropriate model can be challenging due to diverse array of available options and their respective advantages and limitations. In this comprehensive review, we examine four commonly used animal models of TBI: the weight drop, fluid percussion, cortical impact, and blast injury models. Each model is characterized by distinct injury mechanisms, allowing researchers simulate various aspects of TBI pathology. We discuss the unique advantages and disadvantages of each model, providing insights into their applications and considerations for model selection based on research objectives and outcome measures. Furthermore, we highlight emerging directions in TBI modelling, emphasizing the importance of refining and innovating models to replicate the complexity of human TBI. By critically evaluating and understanding the subtlety of different TBI models, researchers can make informed decisions to enhance the translational potential of preclinical TBI research and ultimately improve clinical outcomes for TBI patients.

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“…This injury results in cortical tissue loss and axonal injury, thus representing key characteristics of a focal injury (Osier and Dixon, 2016;Mohamed et al, 2021). Fluid percussion models of TBI produce a diffuse injury in animal model by inflicting a direct fluid pulse onto the intact dura (Dixon et al, 1987;Alder et al, 2011). This injury results in swelling and subcortical neuronal loss, mirroring clinical characteristics of a mixed focal and diffuse injury.…”

Section: Preclinical Models Recapitulate Fundamental Features Of Clin...mentioning

confidence: 99%

Deplete and repeat: microglial CSF1R inhibition and traumatic brain injury

Boland,

Kokiko-Cochran

2024

Front. Cell. Neurosci.

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Traumatic brain injury (TBI) is a public health burden affecting millions of people. Sustained neuroinflammation after TBI is often associated with poor outcome. As a result, increased attention has been placed on the role of immune cells in post-injury recovery. Microglia are highly dynamic after TBI and play a key role in the post-injury neuroinflammatory response. Therefore, microglia represent a malleable post-injury target that could substantially influence long-term outcome after TBI. This review highlights the cell specific role of microglia in TBI pathophysiology. Microglia have been manipulated via genetic deletion, drug inhibition, and pharmacological depletion in various pre-clinical TBI models. Notably, colony stimulating factor 1 (CSF1) and its receptor (CSF1R) have gained much traction in recent years as a pharmacological target on microglia. CSF1R is a transmembrane tyrosine kinase receptor that is essential for microglia proliferation, differentiation, and survival. Small molecule inhibitors targeting CSF1R result in a swift and effective depletion of microglia in rodents. Moreover, discontinuation of the inhibitors is sufficient for microglia repopulation. Attention is placed on summarizing studies that incorporate CSF1R inhibition of microglia. Indeed, microglia depletion affects multiple aspects of TBI pathophysiology, including neuroinflammation, oxidative stress, and functional recovery with measurable influence on astrocytes, peripheral immune cells, and neurons. Taken together, the data highlight an important role for microglia in sustaining neuroinflammation and increasing risk of oxidative stress, which lends to neuronal damage and behavioral deficits chronically after TBI. Ultimately, the insights gained from CSF1R depletion of microglia are critical for understanding the temporospatial role that microglia develop in mediating TBI pathophysiology and recovery.

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Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice

Cited by 8 publications

References 3 publications

Sleep, inflammation, and hemodynamics in rodent models of traumatic brain injury

Sleep, inflammation, and hemodynamics in rodent models of traumatic brain injury

Experimental animal models in traumatic brain injury research: a comprehensive review of methods and outlook

Deplete and repeat: microglial CSF1R inhibition and traumatic brain injury

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