Lateral migration of blood cells and microspheres in two-dimensional Poiseuille flow: A laser-Doppler study

In Vitro Blood Flow Behaviour in Microchannels with Simple and Complex Geometries

“…Blood flow in both microvessels and microchannels has been measured by several measurements techniques such as: double-slit photometric (Nash & Meiselman, 1983), laser-Doppler anemometer (Uijttewaal et al, 1994), video-based methods (Parthasarathi et al, 1999). Although the past research findings have been encouraging, detailed studies on the way blood flow behaves at a microscopic level have been limited by several factors such as poor spatial resolution, difficulty to obtain accurate measurements at such small scales, optical errors arisen from walls of the microvessels, high concentration of blood cells, and difficulty in visualization of results due to insufficient computing power and absence of reliable image analysis techniques.…”

Measurement of Individual Red Blood Cell Motions Under High Hematocrit Conditions Using a Confocal Micro-PTV System

“…The most plausible explanation relies on the formation of a cell-free plasma layer near the wall, with red blood cells (RBCs) tending to migrate toward the center of the microtube. Extensive research ensued on the flow properties of blood in both dilute and concentrated suspensions using several measuring techniques such as double-slit photometry 7,8 , video microscopy and image analysis [4][5][6]9 , laser-Doppler anemometry 10,11 , and particle-measuring methods [12][13][14][15] . Although extensive studies have been conducted on the microhemodynamic behavior of single RBCs in dilute suspensions, their behavior in concentrated suspensions remains poorly understood, in part due to technical limitations.…”

In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system

“…In addition, the non-continuum behavior of blood flow through microvessels leads to complex flow mechanics, which are not yet clearly understood (Goldsmith and Turitto 1986, Secomb 1995, Mchedlishvili & Maeda 2001. Because several important physiological and pathological phenomena occur in the microcirculation, velocity profiles have been intensively studied both in vivo (Einav et al 1975, Tangelder et al 1986, Golster et al 1999, Parthasarathi et al 1999, Nakano et al 2003 and in vitro (Gaehtgens et al 1970, Baker & Wayland 1974, Born et al 1978, Cochrane et al 1981, Gaehtgens 1987, Uijttewaal et al 1994, Alonso et al 1995, Moger et al 2004, Kim & Lee 2006) using several blood flow measuring techniques. Both in vivo and in vitro experiments have played important roles in our understanding of several phenomena in the microcirculation.…”

“…Historically, the most commonly used experimental techniques to study blood flow in the microcirculation have been double-slit photometry (Gaehtgens et al 1970, Baker & Wayland 1974, video microscopy and image analysis (Bugliarello et al 1963, Tangelder et al 1986, Goldsmith and Turitto 1986, Gaehtgens 1987, Alonso et al 1995, Parthasarathi et al 1999, Tsukada 2000, and laserDoppler anemometry (Einav et al 1975, Born et al 1978, Cochrane et al 1981, Uijttewaal et al 1994, Golster et al 1999. However, with these techniques, both spatial resolution and velocity accuracy are unsatisfactory.…”