Lateral motion of fluorescent molecules embedded into cell membranes of clonal myogenic cells, L6, changes upon cell maturation

Abstract

The lateral motion of fluorescent molecules embedded into cell membranes of myogenic cell line, L6, was measured. The motion of S-F-ConA became faster at cell fusion stage, and became slower after fusion. On the other hand, the motion of lipid analog, F18, was not changed at cell fusion stage. However, after fusion when myotubes were formed, the motion of F18 became slower. At cell fusion stage, there was a large variation in the motion of S-F-ConA.This means that at this stage the properties of myoblasts change drastically and rapidly.

Reduced lateral mobility of a fluorescent lipid analog in cell membranes of rat fibroblasts transformed by simian virus 40

“…RESULTS Table 1 shows the lateral diffusion coefficients of F18 embedded into cell membranes of normal and SV40-transformed fibroblasts. These values were close to those of membrane lipids [10,11] and were almost identical to those obtained in other Table 1 Lateral diffusion coefficient and recovery fraction of cells [4][5][6][7]. Therefore, the motion of F18 may represent that of membrane lipids and the membrane fluidity, as described by the lateral mobility of F18, was similar in various cell types.…”

“…In order to obtain more clear information on the dynamic properties of cell membranes, combination of the data of rotational and lateral mobility is required. The lateral mobility of FI8 and S-F-concanavalin A is very sensitive to the structural changes of cell membranes from neuronal cells [4][5][6] and muscle cells [7]. Here we report that the lateral mobility of fluorescent molecules on cell membranes measured by the FPR technique is different depending on probes used: : the lateral mobility of fluorescent fatty acid analog was lower in transformed fibroblasts, whereas, the lateral mobility of S-F-concanavalin A remained unchanged.…”

“…This may be one of the biological reasons why normal fibroblasts have more fluid membranes than transformed ones. Indeed, in myogenic cells, the mobility of the molecules in the cell membranes increased prior to cell fusion when the cells were in close contact [7].…”

Brain tumor induce immunoregulatory dendritic cells in tumor draining lymph nodes that can be targeted by OX40

“…Recently, additional DC subpopulations have been identified both intratumorally and in TdLN as crucial elements playing a role in regulating the immune response (DC1, DC2, DC3, mregDC, moDC) 15,41,42 . Utilizing scRNA-seq in human and mouse, several groups have described a conserved program consistently identified in DC subpopulations 6,43 . By employing combined antibody and targeted scRNA sequencing, we aimed to identify the DC population in TdLN from both SB28 and EG7 models.…”