Lateral organization of biomimetic cell membranes in varying pH conditions

Cooperativity between sodium ions and water molecules facilitates lipid mobility in model cell membranes

“…The membranes were prepared either in Milli-Q water or in a buffer with the addition of 5 mM up to 1.5 M of NaCl. As the pH of the buffer has a prominent effect on the phase separation in lipid membranes, 25 10 mM HEPES buffer was used to keep the pH of the medium constant at pH = 5.2, equal to that of Milli-Q water (see Experimental section in ESI † ). Representative confocal images of the phase-separated SLBs prepared in Milli-Q water as well as in buffers of different composition are shown in Fig.…”

Cooperativity between sodium ions and water molecules facilitates lipid mobility in model cell membranes

“…The membranes were prepared either in Milli Q water or in a buffer with the addition of 5 mM up to 1.5 M of NaCl. As the pH of the buffer has a prominent effect on the phase separation in lipid membranes 25 , 10 mM HEPES buffer was used to keep the pH of the medium constant at pH=5.2, equal to that of Milli Q water (see experimental section). Representative confocal images of the phaseseparated SLBs prepared in Milli Q water as well as in buffers of different composition are shown in Figure 1A.…”

Nanoscale structural response of biomimetic cell membranes to controlled dehydration

“…SLBs were formed using a previously reported method. 39 Briefly, lipid vesicles were diluted 10 times to a final lipid concentration of 1 mM by addition of HEPES buffer. Aliquots containing MLVs were sonicated in a Bransonic 1800 ultrasonic bath for 10 min to generate small unilamellar vesicles (SUVs).…”