Latest advances and perspectives of liquid biopsy for cancer diagnostics driven by microfluidic on-chip assays

Xie, Yibing; Xu, Xiawei; Wang, Jing; Lin, Jie; Ren, Yong; Wu, Aiguo

doi:10.1039/d2lc00837h

Cited by 17 publications

(8 citation statements)

References 132 publications

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“…Due to the widespread adoption of PDMS-on-glass microfluidic devices across the Lab on a Chip community, [145][146][147] there has been considerable interest in the ability to use such systems as a backbone for DLW-printing microstructures for microfluidic applications. Conventional PDMS-on-glass systems, however, present a number of inherent barriers to DLW-printing of microstructures directly inside of the enclosed microchannels.…”

Section: Dlw Inside Enclosed Pdms-on-glass Microfluidic Systemsmentioning

confidence: 99%

Direct laser writing-enabled 3D printing strategies for microfluidic applications

Young,

Xu,

Sarker

et al. 2024

Lab Chip

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Section: Dlw Inside Enclosed Pdms-on-glass Microfluidic Systemsmentioning

confidence: 99%

Direct laser writing-enabled 3D printing strategies for microfluidic applications

Young,

Xu,

Sarker

et al. 2024

Lab Chip

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“…Microfluidic technologies, sometimes referred to as "lab-on-a-chip" provide an opportunity to create devices or chips that could compete with classical techniques in biomedical and chemical research. [149][150][151][152] These low-cost devices can process microliters of solution via capillary channels in a high-throughput manner, thus reducing the requirement for samples and reagents. Importantly, all reactions and washing steps are performed in sealed reaction systems, ideal for preventing cross-contamination.…”

Section: Microfluidics and Lab-on-a-chip Technology-integrating Indiv...mentioning

confidence: 99%

Advanced technologies towards improved HPV diagnostics

Bartosik,

Moranova,

Izadi

et al. 2024

Journal of Medical Virology

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Persistent infection with high‐risk types of human papillomaviruses (HPV) is a major cause of cervical cancer, and an important factor in other malignancies, for example, head and neck cancer. Despite recent progress in screening and vaccination, the incidence and mortality are still relatively high, especially in low‐income countries. The mortality and financial burden associated with the treatment could be decreased if a simple, rapid, and inexpensive technology for HPV testing becomes available, targeting individuals for further monitoring with increased risk of developing cancer. Commercial HPV tests available in the market are often relatively expensive, time‐consuming, and require sophisticated instrumentation, which limits their more widespread utilization. To address these challenges, novel technologies are being implemented also for HPV diagnostics that include for example, isothermal amplification techniques, lateral flow assays, CRISPR‐Cas‐based systems, as well as microfluidics, paperfluidics and lab‐on‐a‐chip devices, ideal for point‐of‐care testing in decentralized settings. In this review, we first evaluate current commercial HPV tests, followed by a description of advanced technologies, explanation of their principles, critical evaluation of their strengths and weaknesses, and suggestions for their possible implementation into medical diagnostics.

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“…For example, nowadays digital microfluidics 17 challenges standard microchannels by providing control over fluids through discrete droplets. Moreover, the integration of microfluidics with electronics, 18 photonics, 19 plasmonic, 20 magnetics, 21 nanomaterials, 22 , 23 and advanced microscopy and spectroscopy techniques 24 enhances the accuracy in fluid manipulation, improves the precision in sensing and characterizing biological samples, and increases the overall automation and screening efficiency.…”

Section: Next Generation Microfluidic Systems: Trendsmentioning

confidence: 99%

Next-Generation Microfluidics for Biomedical Research and Healthcare Applications

Deliorman,

Ali,

Qasaimeh

2023

Biomed Eng Comput Biol

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Microfluidic systems offer versatile biomedical tools and methods to enhance human convenience and health. Advances in these systems enables next-generation microfluidics that integrates automation, manipulation, and smart readout systems, as well as design and three-dimensional (3D) printing for precise production of microchannels and other microstructures rapidly and with great flexibility. These 3D-printed microfluidic platforms not only control the complex fluid behavior for various biomedical applications, but also serve as microconduits for building 3D tissue constructs—an integral component of advanced drug development, toxicity assessment, and accurate disease modeling. Furthermore, the integration of other emerging technologies, such as advanced microscopy and robotics, enables the spatiotemporal manipulation and high-throughput screening of cell physiology within precisely controlled microenvironments. Notably, the portability and high precision automation capabilities in these integrated systems facilitate rapid experimentation and data acquisition to help deepen our understanding of complex biological systems and their behaviors. While certain challenges, including material compatibility, scaling, and standardization still exist, the integration with artificial intelligence, the Internet of Things, smart materials, and miniaturization holds tremendous promise in reshaping traditional microfluidic approaches. This transformative potential, when integrated with advanced technologies, has the potential to revolutionize biomedical research and healthcare applications, ultimately benefiting human health. This review highlights the advances in the field and emphasizes the critical role of the next generation microfluidic systems in advancing biomedical research, point-of-care diagnostics, and healthcare systems.

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Latest advances and perspectives of liquid biopsy for cancer diagnostics driven by microfluidic on-chip assays

Abstract: Microfluidics based lab on a chip technology is a multidisciplinary approach evolving rapidly in past decade and remains hot topic of extensive investigations as a promising microanalysis platform for a...

Cited by 17 publications

References 132 publications

Direct laser writing-enabled 3D printing strategies for microfluidic applications

Direct laser writing-enabled 3D printing strategies for microfluidic applications

Advanced technologies towards improved HPV diagnostics

Next-Generation Microfluidics for Biomedical Research and Healthcare Applications

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