Latest Advances in Protein-Recovery Technologies from Agricultural Waste

Yusree, Farhana Iylia Fatinee Mohd; Peter, Angela Paul; Nor, Mohamed Yusoff Mohd; Show, Pau Loke; Mokhtar, Mohd Noriznan

doi:10.3390/foods10112748

Cited by 18 publications

(6 citation statements)

References 91 publications

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“…Indeed, huge amounts of these wastes are produced every year which necessitate downstream processing for simultaneous valorization of such wastes and eliminating the concomitating adverse effects. Agro-industrial wastes are used for manufacturing of biofuels, enzymes, vitamins, antioxidants, animal feed, antibiotics and other chemicals [10].…”

Section: Discussionmentioning

confidence: 99%

Three-phase partitioning for isolating peroxidase from lemon peels

Al-Madhagi¹,

Valantina²,

Abdelwahed³

2023

Ann Proteom Bioinform

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Background and objective: Peroxidase is an oxidoreductase that uses different compounds as substrates and thus can be utilized for different applications. The goal of this work is to isolate peroxidase from lemon peels using Three-Phase Portioning (TPP). Methods: TPP was set by adding varying amounts of salts and alcohol and the enzyme activity recovery was measured for each variable. Different parameters were optimized successively in order to achieve the highest enzyme activity recovery including salt type, salt concentration, pH, alcohol/crude extract ratio and type of alcohol and then, combining all optimized conditions together. Results: Salt that gave maximal recovery was sodium potassium tartrate, optimal salt concentration was 15%, optimal pH was 8, optimal alcohol/crude extract ratio was 1 and t-butanol was preferred to 1-butanol. Efficiently, upon combining all optimized factors, an activity recovery of 175% was obtained. Conclusion: This protocol provides an easy, feasible method to efficiently isolate peroxidase from lemon peels using TPP.

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Section: Discussionmentioning

confidence: 99%

Three-phase partitioning for isolating peroxidase from lemon peels

Al-Madhagi¹,

Valantina²,

Abdelwahed³

2023

Ann Proteom Bioinform

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“…Extraction typically occurs in buffered media at low temperatures where the extracted proteinases are purified using different methods, such as an aqueous two-phase system, chromatographic separation, reverse micellar system, and membrane filtration. These enzymes are highly important at the industrial level, with an annual sale of approximately $3 billion, owing to their wide application in food industries, pharmaceuticals, and detergent manufacturing [48].…”

Section: Pineapple (Ananas Comosus)mentioning

confidence: 99%

High Added-Value by-Products from Biomass: A Case Study Unveiling Opportunities for Strengthening the Agroindustry Value Chain

Dos Santos,

Barcellos-Silva,

Leite-Barbosa

et al. 2024

Biomass

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The current era witnesses a remarkable advancement in biomass utilization, guided by the principles of green chemistry and biorefinery and the comprehensive exploitation of plant-based raw materials. Predominantly, large-scale production methods have been pursued, akin to approaches in the oil industry, enabling the incorporation of novel products into energy and petrochemical markets. However, the viability of such systems on a small and medium scale is hindered by logistical challenges and the constraints of economies of scale. For small agricultural producers and food processing companies, the complete utilization of biomass transcends environmental responsibility, evolving into a strategy for survival through the diversification of by-products with enhanced value. The state of Rio de Janeiro in Brazil presents a range of population dynamics, geographical features, climate conditions, and agricultural production patterns that closely resemble those found in various tropical countries and agricultural regions worldwide. This region, sustaining a green belt supporting 17 million people, provides an apt case study for investigating chemical compounds with potential value among agro-industrial residues, which can motivate the creation of a lucrative biotechnological industry. Examples include naringenin and hesperidin from oranges and lemons, epi-gallo-catechin gallate from bananas, caffeic acids from coffee, and the bromelain enzyme from pineapples. This study addresses the challenges associated with developing biotechnological alternatives within the agroindustry, considering economic, technological, logistical, and market-related aspects. The insights from examining the Brazilian state of Rio de Janeiro will contribute to the broader discourse on sustainable biomass utilization and the creation of value-added by-products.

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“…These include hydrodynamic cavitation extraction (HCE), microwave assisted extraction (MAE), pulsed electric fields (PEF) and ultrasound assisted extraction (UAE), the working mechanisms of which have been reviewed in-depth in other works. 83–107 Key advantages and drawbacks of these technologies with regards to process operation and product safety/nutrition are summarised in Table 2.…”

Section: Waste-to-protein Sourcesmentioning

confidence: 99%