Latex agglutination test for detection of mutans streptococci in relation to dental caries in children

Takei, T; Ogawa, Taiji; Alaluusua, Satu; Fujiwara, Taku; Morisaki, Ichijiro; Ooshima, Takashi; Sobue, S; Hamada, Shigeyuki

doi:10.1016/0003-9969(92)90004-r

Cited by 5 publications

(4 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The S. mutans oral isolate MT8148 (Ooshima et al, 1983) and a blood isolate TW295 (Fujiwara et al, 2001) were used. In addition, Streptococcus sobrinus 6715 (Takei et al, 1992), Streptococcus sanguinis ATCC 10556, Streptococcus oralis ATCC 10557, Streptococcus gordonii ATCC 10558, Streptococcus mitis ATCC 49456 and Streptococcus salivarius HHT were utilised, along with S. sanguinis strains isolated from the blood of patients with bacteraemia, sepsis or infective endocarditis (Supplemental Table S1) (Nemoto et al, 2011). All strains were grown under 5% CO2 on Mitis-Salivarius (MS) agar (Difco Laboratories, Detroit, MI, USA) plates for 48 h at 37°C or brain heart infusion (BHI) broth (Difco) for 18 h at 37°C.…”

Section: Bacterial Strainsmentioning

confidence: 99%

Aggravation of inflammatory bowel diseases by oral streptococci

et al. 2013

View full text Add to dashboard Cite

OBJECTIVES: Streptococcus mutans can aggravate colitis in mice. We evaluated the virulence of colitis using type strains as well as blood isolates of several oral streptococcal species. MATERIALS AND METHODS: We investigated the susceptibility of blood isolates of several oral streptococci to phagocytosis, adhesion to and invasion of hepatic cells and interferon-c secretion. A mouse model of dextran sodium sulphate-induced colitis was used to evaluate bacterial aggravation of colitis. In addition, interferon-c antibody was administered to mice with prominent aggravation of colitis. RESULTS: In vitro analyses showed that Streptococcus sanguinis ATCC 10556 was a possible virulent strain among type strains of several oral streptococci, and that analysis of blood isolates of S. sanguinis TW289 revealed a potential virulent strain. Intravenous administration of ATCC 10556 and TW289 caused prominent aggravation of dextran sodium sulphate-induced colitis, and histopathological examinations showed that interferon-c secretion due to infection of hepatic cells caused colitis aggravation. Administration of interferon-c antibody suppressed TW289-induced colitis. CONCLUSION: These results suggest that some virulent oral streptococcal strains are associated with the aggravation of colitis induced by enhanced secretion of interferon-c when they invade the bloodstream. Oral Diseases (2014) 20, 359-366

show abstract

Section: Bacterial Strainsmentioning

confidence: 99%

Aggravation of inflammatory bowel diseases by oral streptococci

et al. 2013

View full text Add to dashboard Cite

show abstract

“…It is generally accepted that mutans streptococci and lac tobacilli are associated with dental caries [Loesche, 1986], The presence o f these bacteria in human dental plaque is indicative of an increased cariogenic activity [Krasse,1984;Loesche, 1986;Aaltonen et al" 1987;de Soet et al, 1990b;Takei et al, 1992;Batoni et al, 1992]. The bacterial flora of open carious lesions is complex, with lactobacilli, mutans streptococci, and many other oral microorganisms being isolated from the dentine [Going et al, 1978], We reported recently that the bacterial composition o f HCL is less com plex [Weerheijm et al, 1990], Bacteria other than mutans streptococci and lactobacilli are found only rarely, even when using non-selective blood agar; Actinomyces spp.…”

Section: Discussionmentioning

confidence: 99%

A Comparison of the Microbial Flora in Carious Dentine of Clinically Detectable and Undetectable Occlusal Lesions

Soet¹,

Weerheijm

Amerongen

et al. 1995

Caries Res

View full text Add to dashboard Cite

It is not known whether the aetiology of occlusal hidden caries lesions (HCL) is identical to that of small visible lesions (SVL). Previous studies of the microflora of HCL suggest that relatively few species can be isolated. The aim of the present study was to compare the bacterial composition of dentine from 10 HCL and 17 SVL in a population of children aged 8-18 years. The following bacteria were identified: Actinomyces spp., mutans streptococci, Streptococcus sanguis, Streptococcus oralis, Streptococcus gordonii, Streptococcus mitis, and Lactobacillus spp. Streptococcus mutans was found more often in the HCL group (p = 0.03), while Streptococcus sobrinus was found more often in the SVL group (p = 0.05). However, proportions of both species were lower in the SVL group as compared with the HCL group (S. mutans p = 0.07; S. sobrinus p = 0.03). Lactobacilli and Actinomyces spp. were found at similar levels in both groups. A more diverse range of streptococcal species and Veillonella was found in the SVL group (p = 0.001). These results show a less complex microflora in HCL as compared with SVL, indicating that the aetiology of hidden caries might be different from that of open caries.

show abstract

“…Streptococcus mutans strains and culture media Table 1 lists the S. mutans strains used in the present study. A total of 14 S. mutans clinical strains were selected from our laboratory stock (six positive for Cnm, four positive for Cbm, four negative for both Cnm and Cbm) (Ooshima et al, 1983;Takei et al, 1992;Fujiwara et al, 2001;Nakano et al, 2004aNakano et al, , 2008Nakano et al, , 2010bNomura et al, 2012). As for Cnm-positive and Cbm-positive strains, isogenic mutant strains with inactivated cnm or cbm genes constructed following erythromycin-resistant genes insertions were also utilized (Nakano et al, 2010b;Nomura et al, 2012).…”

Section: Methodsmentioning

confidence: 99%