Latex–protein complexes from an acute phase recombinant antigen of Toxoplasma gondii for the diagnosis of recently acquired toxoplasmosis

Peretti, Leandro Ezequiel; Gonzalez, Veronica D.; Marcipar, Iván Sergio; Gugliotta, Luis Marcelino

doi:10.1016/j.colsurfb.2014.05.009

Cited by 5 publications

(4 citation statements)

References 41 publications

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“…The best performance was obtained with the complexes PS-GMA I/P22Ag (Γ = 2•17 mg m −2 ) and PS-MAA II/P22Ag (Γ = 2•93 mg m −2 ), which produced OD ac /OD neg = 4•79 and 2•18, respectively; and OD ac /OD ch = 4•79 and 1•66, respectively. In all cases, the response obtained with the different LPC were similar to those obtained with the single antigen (Peretti et al 2014), thus confirming that the antigen was not affected during the sensitization process, and consequently the produced LPC were able to recognize specific antibodies.…”

Section: R E S U L T S a N D Discussionsupporting

confidence: 65%

“…In all cases, the response obtained with the different LPC were similar to those obtained with the single antigen (Peretti et al . 2014), thus confirming that the antigen was not affected during the sensitization process, and consequently the produced LPC were able to recognize specific antibodies.…”

Section: Resultsmentioning

confidence: 54%

“…Then, microplates were washed thrice with Tween in PBS at 0·01%, and incubated with a 1:2000 dilution of the Peroxidase-conjugated goat anti-human IgG, Fc γ (Zymed, San Francisco, USA) in skimmed milk in PBS at 1% (v/v). The enzymatic reaction was developed using 100 µ L of tetramethyl benzidine (Zymed) in H 2 O 2 and stopped with 100 µ L of 2 N H 2 SO 4 (Peretti et al 2014).…”

Section: Methodsmentioning

confidence: 99%

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Diagnosis of toxoplasmosis in pregnancy. Evaluation of latex–protein complexes by immnunoagglutination

et al. 2017

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The aim of this work was to obtain a reagent based on latex particles for ruling out acute toxoplasmosis in pregnant women by immunoagglutination (IA). Latex-protein complexes (LPC) were previously synthesized coupling the recombinant protein of Toxoplasma gondii P22Ag and the homogenate of the parasite to latex particles with different size, chemical functionality and charge density. LPC were tested in IA assays against a panel of 72 pregnant women serum samples. Results were analysed through receiver operating characteristic curves, determining area under the curve (AUC), sensitivity, specificity positive and negative predictive values (PPV and NPV, respectively). It was observed that the antigenicity of proteins was not affected during sensitization by either physical adsorption or covalent coupling. The best results in the sense of maximizing discrimination of low avidity sera from chronic ones were observed for the IA test based on latex particles with carboxyl functionality and the recombinant P22Ag, obtaining an AUC of 0·94, a sensitivity of 100% and a NPV of 100%. In this way, the proposed test could be useful for the toxoplasmosis diagnosis in pregnant women, with the advantages of being cheap, rapid and easy to be implemented.

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Section: R E S U L T S a N D Discussionsupporting

confidence: 65%

Section: Resultsmentioning

confidence: 54%

Section: Methodsmentioning

confidence: 99%

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Diagnosis of toxoplasmosis in pregnancy. Evaluation of latex–protein complexes by immnunoagglutination

et al. 2017

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“…Polymeric latex particles for biomedical purposes are of great interest because of their variety of applications (Pichot et al 1997), which include the production of a latex agglutination test (LAT) for the detection of illnesses like Chagas' disease (Gonzalez et al 2008b(Gonzalez et al , 2010García et al 2013García et al , 2014, cystic echinococcosis (Miranda et al 2009), bluetongue virus (Yang et al 2010), neosporosis (Moraveji et al 2012), toxoplasmosis (Jiang et al 2008;Peretti et al 2014Peretti et al , 2016, leptospirosis (Prochetto et al 2017) and leishmaniasis (García et al 2017), among others. Leptospirosis is a zoonotic disease caused by the bacterium Leptospira interrogans which affects humans and a wide range of animals (Vanasco et al 2008;Hartskeerl et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination

Peretti

Gonzalez

Clementi

et al. 2020

Braz. J. Chem. Eng.

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Synthesis and characterization of latex-protein complexes from different antigens ofToxoplasma gondiifor immunoagglutination assays

Peretti

Gonzalez

Costa

et al. 2016

International Journal of Polymeric Materials and Polymeric Biom

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The acute phase recombinant protein of Toxoplasma gondii P22Ag was expressed and purified and the homogenate of the parasite was obtained from an infected mouse. These antigens were used to produce latex-protein complexes (LPC) through physical adsorption and chemical coupling onto different latexes, with the aim of producing immunoagglutination (IA) reagents able to detect recently acquired toxoplasmosis. Polystyrene and "core-shell" latexes where employed, exhibiting varied particle size, functionality (carboxyl or epoxy), and charge density. In sensitization experiments for producing LPC, the recombinant protein showed better coupling efficiency onto the particles surface than the homogenate and this could be explained by the complex mixture of the homogenate, which includes a large number of proteins of different molecular mass, isoelectric points, and hydrophobicity. The synthesized LPC were employed in IA assays. To this effect, the agglutination reaction was followed by measuring the changes in the optical absorbance by turbidimetry. Experiments against control sera were performed to evaluate the performance of various LPC and it was observed that the IA test based on P22Ag and the carboxylated latex of 350 nm of particle diameter allowed a good discrimination between acute sera and chronic/negative ones. The proposed test is cheap, rapid, and easy to implement.

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Latex–protein complexes from an acute phase recombinant antigen of Toxoplasma gondii for the diagnosis of recently acquired toxoplasmosis

Cited by 5 publications

References 41 publications

Diagnosis of toxoplasmosis in pregnancy. Evaluation of latex–protein complexes by immnunoagglutination

Diagnosis of toxoplasmosis in pregnancy. Evaluation of latex–protein complexes by immnunoagglutination

Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination

Synthesis and characterization of latex-protein complexes from different antigens ofToxoplasma gondiifor immunoagglutination assays

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