Lauric acid abolishes interferon-gamma (IFN-γ)-induction of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression in human macrophages

Lim, Wei-Siong; Gan, Mary-Shi-Ying; Ong, Melissa-Hui-Ling; Chew, Choy-Hoong

doi:10.1016/j.apjr.2015.06.005

Cited by 14 publications

(8 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Resazurin metabolism of RAW macrophages was only marginally affected by fatty acid treatments, with a quadratic effect found with glycerol monolaurate. Past studies have found no effect of lauric acid on macrophage cell viability at lower concentrations (less than 20 μmol/L for 24 h incubation) [ 49 ], although another study with a 48 h incubation period found cytotoxic effects at greater concentrations (400 μmol/L) [ 50 ]. Judging from these studies, lauric acid likely impacts cell viability but only at greater concentrations with longer incubation times than those used in the present study.…”

Section: Discussionmentioning

confidence: 99%

Development of an in vitro macrophage screening system on the immunomodulating effects of feed components

Sivinski

Mamedova

Rusk

et al. 2020

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background While feed components capable of modulating the immune system are highly sought after and marketed, often little evidence is available to support functional immune response claims. Thus, a high-throughput in vitro cell screening system was developed to test these compounds for innate immune signaling effects, using Saccharomyces cerevisiae and its cell wall components in addition to lauric acid and its esters as models in two separate experiments. This screening system utilized RAW 264.7 murine macrophages to assess live S. cerevisiae cells and S. cerevisiae -derived cell wall components β-glucan, mannan, and zymosan (a crude cell wall preparation containing both β-glucan and mannan). D -mannose was also evaluated as the monomer of mannan. We also examined the effect of a saturated fatty acid (C12:0, lauric acid) and its esters (methyl laurate and glycerol monolaurate) on innate immune cell activation and cellular metabolism. RAW cells were transfected with a vector that drives expression of alkaline phosphatase upon promoter activation of nuclear factor κ-light-chain-enhancer of activated B cells (NFκB), a major inflammatory/immune transcription factor. RAW cells were incubated with 0.01, 0.1 or 1 mg/mL of yeast compounds alone or RAW cells were challenged with LPS and then incubated with yeast compounds. In a separate experiment, RAW cells were incubated with 0, 0.5, 2.5, 12.5, 62.5, and 312.5 μmol/L of lauric acid, methyl laurate, or glycerol monolaurate alone, or RAW cells were challenged with LPS and then incubated with fatty acid treatments. Results Treatment with zymosan or β-glucan alone induced NFκB activation in a dose-dependent manner, whereas treatment with D -mannose, mannan, or live S. cerevisiae cells did not. Post-treatment with mannan after an LPS challenge decreased NFκB activation, suggesting that this treatment may ameliorate LPS-induced inflammation. Slight increases in NFκB activation were found when fatty acid treatments were applied in the absence of LPS, yet substantial reductions in NFκB activation were seen when treatments were applied following an LPS challenge. Conclusions Overall, this cell screening system using RAW macrophages was effective, high-throughput, and sensitive to feed components combined with LPS challenges, indicating modulation of innate immune signaling in vitro . Graphical abstract

show abstract

Section: Discussionmentioning

confidence: 99%

Development of an in vitro macrophage screening system on the immunomodulating effects of feed components

Sivinski

Mamedova

Rusk

et al. 2020

J Animal Sci Biotechnol

View full text Add to dashboard Cite

show abstract

“…Wei-Siong et al [25] study, an ICAM expression was up-regulated by IFN through nuclear factor kappa B (NF interferon regulatory factor expression.…”

Section: Discussionmentioning

confidence: 98%

In vitro: The Modulating Effect of Myricetin on the Atherosclerosis Related Processes in THP1 Macrophages

Almassabi

Huwait²,

Almowallad

et al. 2021

JPRI

View full text Add to dashboard Cite

Aims: To assess the anti-atherosclerotic effects of Myricetin (pharmaceutical) in human THP-1 macrophages following IFN-γ or MCP-1 stimulation. Study Design: The protective effects of myricetin against atherosclerosis was evaluated using the humanTHP-1 macrophages and studying the following parameters namely, cell viability, cell proliferation, cell migration, inflammation related gene expression and cholesterol efflux in vitro. Place and Duration of Study: THP-1 cell line: Department of biochemistry (faculty of science), Cell Culture Unit, Experimental Biochemistry Unit (King Fahad Medical Research Centre), King Abdul Aziz University, between September 2019 and September 2020. Methodology: The THP-1 cell lines were differentiated into macrophages by incubation with PMA (160 nM) for 24 hours. The viability percentage was determined using Pierce LDH cytotoxicity assay kit, the percentage change in macrophages proliferation was evaluated by crystal violet dye, the RNA was extracted then the cDNA was synthesized and the quantitative real time polymerase chain reaction (qRT-PCR) was done for inflammation-related genes, ICAM-1 and MCP-1. The percentage of monocyte migration and cholesterol efflux were also calculated. Results: Cytotoxicity assays demonstrated no significant toxicity with myricetin at 25μM and 50 μM concentrations on THP-1 macrophages. The quantitative real-time RT-PCR (qRT-PCR) demonstrated a significant increase in interferon gamma (IFN-γ) mediated expression of both intercellular adhesion molecule (ICAM-1) and monocyte chemo-attractant protein-1 (MCP-1) by 2.1 and 7.1 fold respectively, compared to the control. Treatment with myricetin (25 μM and 50 μM) significantly inhibited the IFN-γ induced overexpression of ICAM-1 by 42.86% & 71.34% and MCP-1 by 53.52% & 87.32% respectively. Myricetin (25 μM) significantly reduced the migration of monocytes by 33.66% compared to MCP-1. The cholesterol efflux from THP-1 macrophages treated with myricetin was significantly increased by 47% and 57% in the absence and presence of IFN-γ, respectively compared to the control. Conclusion: Myricetin has anti-inflammatory effects and supports cholesterol efflux, which can help in prevention of atherosclerosis. Furthermore, myricetin did not exhibit any cytotoxic effects and therefore is a safe phytochemical which can complement conventional therapeutics.

show abstract

“…Lauric acid is a free saturated fatty acid in serum [24]. After a large amount of lauric acid is injected into the blood vessels, the blood vessels deposited in the corresponding blood vessels, which will cause vascular endothelial cells and vascular wall injury, induce thrombosis, cause local vascular circulation disorder, and eventually form the ischemic state of the local organism [25,26]. At present, lauric acid has been widely used to induce TAO model [21].…”

Section: Discussionmentioning

confidence: 99%

Integration Traditional Chinese Medicine and Nibble Debridement and Dressing Method Reduce Thrombosis and Inflammatory Response in the Treatment of Thromboangiitis Obliterans

Zhong

Huang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Thromboangiitis obliterans (TAO), also known as Buerger's disease, is an occlusive arterial disease. However, the pathogenesis of TAO is still unclear. Research has shown that traditional Chinese medicine (TCM) has significant advantages in the treatment of TAO. Our purpose is to explore the underlying roles of TCM in combination with nibble debridement and dressing method (NDDM) in TAO rat model.Methods: 10 mg/ml sodium laurate was utilized to establish a TAO rat model, and then the TAO mode rats were treated with notoginseng powder (NP), maifusheng (MFS) or the combination of NP or MFS and NDDM. Ganrene’s classification and, blood rheology was evaluated; the pathological characteristics of rat limbs were examined by H&E staining and Masson staining; CD3+ and CD20+ levels were measured by immunohistochemistry and flow cytometry. In addition, inflammation-associated cytokines were analyzed by RT-qPCR, Western blot and ELISA assays. Results: ntegration NP or MFS and NDDM dramatically reduced the ganrene’s classification and affected blood rheology parameter of TAO model rats compared with NP and MFS alone. Meanwhile, NP or MFS in combination with NDDM decreased CD3+CD20+T cells, reduced thrombosis and inflammatory cell infiltration, and dramatically decreased the levels of inflammation-associated cytokines.Conclusion: Our results suggested that integration NP or MFS and NDDM could relieve the symptoms of TAO model rats induced by sodium laurate, which might provide new management strategy for TAO.

show abstract

Lauric acid abolishes interferon-gamma (IFN-γ)-induction of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression in human macrophages

Cited by 14 publications

References 24 publications

Development of an in vitro macrophage screening system on the immunomodulating effects of feed components

Development of an in vitro macrophage screening system on the immunomodulating effects of feed components

In vitro: The Modulating Effect of Myricetin on the Atherosclerosis Related Processes in THP1 Macrophages

Integration Traditional Chinese Medicine and Nibble Debridement and Dressing Method Reduce Thrombosis and Inflammatory Response in the Treatment of Thromboangiitis Obliterans

Contact Info

Product

Resources

About