Lavage and nipple aspiration of breast ductal fluids: A source of biomarkers for environmental mutagenesis

Klein, Pamela; Lawrence, Julia

doi:10.1002/em.10061

Cited by 19 publications

(16 citation statements)

References 33 publications

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“…Breast fluids can be obtained either by gentle suction with a simple syringeadapted apparatus and massage (nipple aspiration fluid, NAF) or by microcatheter insertion and flushing (ductal lavage) [1]. Although fluids are not always cellular, particularly in case of NAF, certain cellular characteristics of breast fluids have been proven to predict risk of breast cancer.…”

Section: Introductionmentioning

confidence: 99%

Nutritional predictors for cellular nipple aspirate fluid: Nutrition and Breast Health Study

Kato

Ren

Visscher

et al. 2005

Breast Cancer Res Treat

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The presence of epithelial cells in breast nipple aspirate fluid (NAF), irrespective of abnormality, has been associated with increased risk of breast cancer in previous studies. We sought to investigate whether the presence of epithelial cells in NAF is associated with nutritional parameters among 71 healthy premenopausal women who participated in the Nutrition and Breast Health Study and provided any samples of NAF during the study. Total of 142 samples which were obtained over a 1-year period of intervention with low-fat and/or high vegetable-fruit diets were available for cytological evaluation. The odds ratios (ORs) and 95% confidence intervals (CIs) for the detection of epithelial cells in NAF were estimated by fitting generalized estimating equations models by quartile level of nutritional parameters. The probability of yielding epithelial cell-positive NAF progressively increased with increasing total fat intake (p=0.001). The OR for the highest quartile level of fat intake, compared with lowest, was 7.22 (95% CI 1.14-45.82). On the other hand, there were a marginally significant inverse association with total fiber intake as well as an weak inverse association with the number of servings of fruit and vegetables. Furthermore, the probability of detecting epithelial cells in NAF decreased with increasing plasma levels of lutein and alpha-carotene (p-values for linear trend; 0.001 and 0.049, respectively). The ORs for the highest versus lowest quartile levels are 0.17 (95% CI 0.04-0.65) and 0.19 (95% CI 0.04-0.91), respectively. These results are generally in support of roles of nutritional factors in breast cancer and thus further studies are warranted.

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Section: Introductionmentioning

confidence: 99%

Nutritional predictors for cellular nipple aspirate fluid: Nutrition and Breast Health Study

Kato

Ren

Visscher

et al. 2005

Breast Cancer Res Treat

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“…[33][34][35] Amongst the breast cancers, detection of tumor suppressor gene hypermethylation in serum, plasma, fine needle aspirate, nipple aspirate and ductal lavage has been reported. 18,19,[36][37][38][39][40][41] We have initiated a study aimed at detecting gene hypermethylation from serum derived circulating DNA and assessed its concordance with methylation status of the same gene in matched tumors from breast cancer patients. For our preliminary study, we chose two genes RASSF1A and RARβ and checked their methylation status in breast tumors and matched sera along with representative normal breast tissues and sera.…”

Section: Introductionmentioning

confidence: 99%

Detection of RASSF1A and RAR? Hypermethylation in Serum DNA from Breast Cancer Patients

Shukla

Mirza²,

Sharma³

et al. 2006

Epigenetics

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ABBREVIATIONS RASSF1ARAS association domain family protein 1A RARβ retionic acid receptor β MSP methylation specific PCR ERα estrogen receptor α PRB progesterone receptor B ACKNOWLEDGEMENTSWe are grateful to Department of Biotechnology, India, for providing the financial support to conduct this study. S.M. is grateful to University Grant Commission for award of Junior Research Fellowship. The authors thank Dr. Cecile Rochette Egly for the kind gift of RARβ monoclonal antibody. Research PaperDetection of RASSF1A and RARβ Hypermethylation in Serum DNA from Breast Cancer Patients ABSTRACTBreast cancer is fast emerging as the leading cancer amongst females, especially in young females in metropolitan cities in India. The epigenetic alterations involved in the onset and progression of breast cancer may serve as biomarkers for early detection and prognosis of the disease. Furthermore, using body fluids such as serum offers a noninvasive method to procure multiple samples for such analyses. In this study, we examined methylation status of two normally unmethylated but biologically significant cancer genes, RAS association domain family protein 1A (RASSF1A) and retionic acid receptor β (RARβ) by methylation specific PCR (MSP) in invasive ductal carcinomas of the breast and paired serum DNA. RASSF1A was found to be methylated in 17 of 20 (85%) breast tumors; while sera from 15 of 20 (75%) of the patients showed concordant methylated RASSF1A, with a sensitivity of 88%. RARβ was methylated in 2/20 (10%) breast tumors. A gene unmethylated in the tumor DNA was always found to be unmethylated in the matched serum DNA for both RASSF1A and RARβ genes; hence specificity was 100%. Immunohistochemical analysis of RARβ protein in 15 breast carcinoma patients harboring unmethylated RARβ in tumors and serum DNA showed the expression of RARβ protein in tumors and paired normal breast tissues, confirming the MSP findings, suggesting that RARβ promoter is functional in these cases. This study underscores the potential utility of DNA methylation based screening of serum, a readily accessible body fluid, as a surrogate marker for early detection of breast cancer.

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“…Despite the first elucidation of cell-free nucleic acids (cf-NAs) in human bloods early in 1948 [38], it is not until 1990s that research on circulating cf-NAs starts to be prosperous. Moreover, epigenetic biomarkers were also detectable in other body fluids such as nipple aspirate [39,40], urine [41,42], sputum [43] and bronchoalveolar lavage [44]. …”

Section: Methylated Dna As Biomarkermentioning

confidence: 99%

Methylated DNA and microRNA in Body Fluids as Biomarkers for Cancer Detection

Wang

Jin

2013

IJMS

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Epigenetic alterations including DNA methylation and microRNAs (miRNAs) play important roles in the initiation and progression of human cancers. As the extensively studied epigenetic changes in tumors, DNA methylation and miRNAs are the most potential epigenetic biomarkers for cancer diagnosis. After the identification of circulating cell-free nuclear acids, increasing evidence demonstrated great potential of cell-free epigenetic biomarkers in the blood or other body fluids for cancer detection.

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Lavage and nipple aspiration of breast ductal fluids: A source of biomarkers for environmental mutagenesis

Cited by 19 publications

References 33 publications

Nutritional predictors for cellular nipple aspirate fluid: Nutrition and Breast Health Study

Nutritional predictors for cellular nipple aspirate fluid: Nutrition and Breast Health Study

Detection of RASSF1A and RAR? Hypermethylation in Serum DNA from Breast Cancer Patients

Methylated DNA and microRNA in Body Fluids as Biomarkers for Cancer Detection

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