Lawn, turfgrass and pasture applications.

Grewal, P. S.; Koppenhöfer, Albrecht M.; Choo, Ho Yul; Ehlers, R. U.; Shapiro‐Ilan, David I.

doi:10.1079/9780851990170.0115

Cited by 85 publications

(62 citation statements)

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“…The nematodes actively seek out and infect insect hosts by entering through natural openings or burrowing directly through the insect cuticle (13). The host range of these nematodes includes grubs, weevils, ticks, cockroaches, crickets, and the larval stage of many insects (14). Once inside the insect, the nematodes regurgitate the bacteria into the hemolymph (10).…”

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confidence: 99%

Elucidation of the Photorhabdus temperata Genome and Generation of a Transposon Mutant Library To Identify Motility Mutants Altered in Pathogenesis

Hurst¹,

Rowedder²,

Michaels³

et al. 2015

J. Bacteriol.

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The entomopathogenic nematode Heterorhabditis bacteriophora forms a specific mutualistic association with its bacterial partner Photorhabdus temperata. The microbial symbiont is required for nematode growth and development, and symbiont recognition is strain specific. The aim of this study was to sequence the genome of P. temperata and identify genes that plays a role in the pathogenesis of the Photorhabdus-Heterorhabditis symbiosis. A draft genome sequence of P. temperata strain NC19 was generated. The 5.2-Mb genome was organized into 17 scaffolds and contained 4,808 coding sequences (CDS). A genetic approach was also pursued to identify mutants with altered motility. A bank of 10,000 P. temperata transposon mutants was generated and screened for altered motility patterns. Five classes of motility mutants were identified: (i) nonmotile mutants, (ii) mutants with defective or aberrant swimming motility, (iii) mutant swimmers that do not require NaCl or KCl, (iv) hyperswimmer mutants that swim at an accelerated rate, and (v) hyperswarmer mutants that are able to swarm on the surface of 1.25% agar. The transposon insertion sites for these mutants were identified and used to investigate other physiological properties, including insect pathogenesis. The motility-defective mutant P13-7 had an insertion in the RNase II gene and showed reduced virulence and production of extracellular factors. Genetic complementation of this mutant restored wild-type activity. These results demonstrate a role for RNA turnover in insect pathogenesis and other physiological functions. IMPORTANCEThe relationship between Photorhabdus and entomopathogenic nematode Heterorhabditis represents a well-known mutualistic system that has potential as a biological control agent. The elucidation of the genome of the bacterial partner and role that RNase II plays in its life cycle has provided a greater understanding of Photorhabdus as both an insect pathogen and a nematode symbiont.

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confidence: 99%

Elucidation of the Photorhabdus temperata Genome and Generation of a Transposon Mutant Library To Identify Motility Mutants Altered in Pathogenesis

Hurst¹,

Rowedder²,

Michaels³

et al. 2015

J. Bacteriol.

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“…EPNs have been applied as biological control agents to suppress a variety of economically important insect pests (Kaya and Gaugler, 1993;Hazır et al, 2003;Grewal et al, 2005). In our study, we used the bait insect G. mellonella to establish whether different application methods affected efficacy in the laboratory and field.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of the entomopathogenic nematode Heterorhabditis bacteriophora using different application methods in the presence or absence of a natural enemy

Raja¹,

Hazir²,

Gümüş³

et al. 2015

Turk J Agric For

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IntroductionEntomopathogenic nematodes (EPNs) (Steinernematidae and Heterorhabditidae) are mutualistically associated with insect-pathogenic bacteria and together they kill their insect hosts. The steinernematids are associated with the bacterial genus Xenorhabdus, whereas the heterorhabditids are associated with the genus Photorhabdus. These EPNs have adapted specific mechanisms to transmit the bacteria to their insect hosts (Dillman et al., 2012) and are considered good candidates for integrated pest management of soil insect pests (Lacey and Georgis, 2012). In fact, several nematode species are produced commercially and applied in a variety of cropping systems in many different countries (Alves, 1986;Garcia et al., 2008). These biological control agents must be delivered in a way that enables the infective juveniles (IJs) of the nematodes to survive and infect their hosts (Shapiro-Ilan et al., 2006;Brusselman et al., 2012). EPNs can be applied with nearly all agronomic or horticultural ground equipment including pressurized tank sprayers, mist blowers, electrostatic sprayers, drip irrigation systems, or even aerial sprayers (Georgis, 1990;

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“…Estes organismos também têm sido utilizados em diversas regiões do mundo, no controle de larvas de escarabeídeos e de outros insetos (Grewal et al 2005).…”

Section: Keyunclassified

Eficiência de nematoides entomopatogênicos e inseticidas químicos contra Sphenophorus levis e Leucothyreus sp. em cana-de-açúcar

Leite

Tavares

Botelho

et al. 2012

Pesqui. Agropecu. Trop.

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Lawn, turfgrass and pasture applications.

Cited by 85 publications

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Elucidation of the Photorhabdus temperata Genome and Generation of a Transposon Mutant Library To Identify Motility Mutants Altered in Pathogenesis

Elucidation of the Photorhabdus temperata Genome and Generation of a Transposon Mutant Library To Identify Motility Mutants Altered in Pathogenesis

Efficacy of the entomopathogenic nematode Heterorhabditis bacteriophora using different application methods in the presence or absence of a natural enemy

Eficiência de nematoides entomopatogênicos e inseticidas químicos contra Sphenophorus levis e Leucothyreus sp. em cana-de-açúcar

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