Layer-by-layer assembly of silica nanoparticles on 3D fibrous scaffolds: Enhancement of osteoblast cell adhesion, proliferation, and differentiation

Abstract

Silica nanoparticles were applied onto the fiber surface of an interbonded three-dimensional polycaprolactone fibrous tissue scaffold by an electrostatic layer-by-layer self-assembly technique. The nanoparticle layer was found to improve the fiber wettability and surface roughness. Osteoblast cells were cultured on the fibrous scaffolds to evaluate the biological compatibility. The silica nanoparticle coated scaffold showed enhanced cell attachment, proliferation, and alkaline phosphatase activities. The overall results suggested that interbonded fibrous scaffold with silica nanoparticulate coating could be a promising scaffolding candidate for various applications in bone repair and regeneration.

Surface Micro‐ and Nanoengineering: Applications of Layer‐by‐Layer Technology as a Versatile Tool to Control Cellular Behavior

“…C4) Mean cell number as a function of culture time on different scaffolds. At day 3, samples of 1 layer, 3 layers, and 5 layers were all significantly different from 3D control ( p < 0.05); at day 7, sample of 1 layer was significantly different from 3D control ( p < 0.05) …”

“…Varying the number of poly(allylamine hydrochloride) (PAH)/SiO 2 bilayers, increased the number of nanoparticles per area unit, resulting in different topographies (Figure C1,C1',C2,C2',C3,C3'). Overall, the presence of nanosized silica particles showed enhanced cell attachment and proliferation compared with unmodified 3D polycaprolactone fibrous tissue scaffold—see Figure C4. After 3 d in culture, less interspace between silica nanoparticles seemed to result in less osteoblastic proliferation rates (Figure C4).…”

The role of surface functionalization of silica nanoparticles for bioimaging

“…104 Another application of this type of electrostatic interactions is the layer-by-layer (LbL) method, where alternating layers of cationic and anionic polyelectrolytes are coupled to SNP surface to mediate the interaction with charged biomolecules. 103,105,106 Another type of SNP surface functionalization takes advantage of the strong binding a±nity between streptavidin and biotin, which has been a type of conjugate intensively used in several bioanalytical protocols. 107 Corricelli et al have explored this type of noncovalent binding to produce colloidal stable core-shell PbS/SiO2 NP in physiological media (Fig.…”

Mesoporous silica particle-PLA–PANI hybrid scaffolds for cell-directed intracellular drug delivery and tissue vascularization

“…13 The Notch signalling pathway is a key regulator of stem cell function and a promising therapeutic target in regenerative medicine and developmental disorders, [13][14][15][16][17] but Notch signalling is difficult to control due to its biphasic activity profile and adverse effects upon untargeted delivery of Notch inhibitors. [26][27][28][29] However, none of these materials were designed for cell-directed delivery and intracellular drug release. 13 MSNs have a large intrinsic pore volume, can carry a wide range of drug modalities and have a high flexibility allowing control over the diameter, shape, charge and surface chemistry to regulate bio-behaviour, drug delivery and release properties.…”