Layer-by-layer bioassembly of cellularized polylactic acid porous membranes for bone tissue engineering

Gudurić, Vera; Metz, Carole; Siadous, Robin; Bareille, Reine; Levato, Riccardo; Engel, Elisabeth; Fricain, Jean-Christophe; Devillard, Raphaël; Lužanin, Ognjan; Catros, Sylvain

doi:10.1007/s10856-017-5887-6

Cited by 39 publications

(33 citation statements)

References 43 publications

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“…Control massive scaffolds were 2 mm thick, to reproduce the thickness of the four membranes stacked. We have also shown in one previous study that it was possible for cells to migrate between layers when four membranes were superposed (Guduric et al, ).…”

Section: Methodssupporting

confidence: 73%

“…Another group of authors have used Matrigel © to glue layers to each other, but it did not enable sufficient stabilization (Yang et al, ). In previous experiments, we have used glass rings on the top layer of LBL assemblies to limit the movements of layers during in vitro cell culture, but the glass rings damaged the top layer and the manipulation was difficult because the rings were not completely stable; moreover, it was difficult to manipulate these LBL scaffolds for in vivo implantation (Guduric et al, ). In this study we have used a novel stabilization system, which is easy to fabricate and can be manipulated without damages during in vivo implantations.…”

Section: Discussionmentioning

confidence: 99%

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Layer‐by‐layer bioassembly of poly(lactic) acid membranes loaded with coculture of HBMSCs and EPCs improves vascularization in vivo

Gudurić

Siadous

Babilotte

et al. 2019

J Biomedical Materials Res

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Layer‐by‐layer (LBL) BioAssembly method was developed to enhance the control of cell distribution within 3D scaffolds for tissue engineering applications. The objective of this study was to evaluate in vivo the development of blood vessels within LBL bioassembled membranes seeded with human primary cells, and to compare it to cellularized massive scaffolds. Poly(lactic) acid (PLA) membranes fabricated by fused deposition modeling were seeded with monocultures of human bone marrow stromal cells or with cocultures of these cells and endothelial progenitor cells. Then, four cellularized membranes were assembled in LBL constructs. Early osteoblastic and endothelial cell differentiation markers, alkaline phosphatase, and von Willebrand's factor, were expressed in all layers of assemblies in homogenous manner. The same kind of LBL assemblies as well as cellularized massive scaffolds was implanted subcutaneously in mice. Human cells were observed in all scaffolds seeded with cells, but not in the inner parts of massive scaffolds. There were significantly more blood vessels observed in LBL bioassemblies seeded with cocultures compared to all other samples. LBL bioassembly of PLA membranes seeded with a coculture of human cells is an efficient method to obtain homogenous cell distribution and blood vessel formation within the entire volume of a 3D composite scaffold.

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Section: Methodssupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Layer‐by‐layer bioassembly of poly(lactic) acid membranes loaded with coculture of HBMSCs and EPCs improves vascularization in vivo

Gudurić

Siadous

Babilotte

et al. 2019

J Biomedical Materials Res

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“…The success of a cell‐populated scaffold implantation depends on two mains parameters: scaffold design and cell incorporation . To date, two methods of incorporating cells into scaffolds are being explored: (a) seeding of cells onto the surface of the scaffold following fabrication (top‐down approach) and (b) the incorporation of cells into the scaffold fabrication process (bottom‐up approach).…”

Section: Discussionmentioning

confidence: 99%

“…To date, two methods of incorporating cells into scaffolds are being explored: (a) seeding of cells onto the surface of the scaffold following fabrication (top‐down approach) and (b) the incorporation of cells into the scaffold fabrication process (bottom‐up approach). The small seeded PLA scaffolds produced in this study could be assembled to produce a larger volume scaffold like in the bottom up tissue engineering approach to achieve a homogeneous cells distribution in the final 3D construct . Regardless of the method used to add cells to the 3D scaffolds, vascularization remains a great challenge in tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

“…Another advantage of FDM technology is the ease to associate cells with these thin polymeric scaffolds resulting in a better cell colonization, proliferation and differentiation compared with a larger 3D structure which often includes an inner hypoxic central area, which prevents deep cell colonization. Moreover, stacked together, these populated scaffolds can form a large 3D structure within an internal organization improving both cell communication and cell–material interactions in vitro and in vivo …”

Section: Introductionmentioning

confidence: 99%

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Characterization of printed PLA scaffolds for bone tissue engineering

Grémare

Gudurić

Bareille

et al. 2017

J Biomedical Materials Res

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278

180

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Autografts remain the gold standard for orthopedic transplantations. However, to overcome its limitations, bone tissue engineering proposes new strategies. This includes the development of new biomaterials such as synthetic polymers, to serve as scaffold for tissue production. The objective of this present study was to produce poly(lactic) acid (PLA) scaffolds of different pore size using fused deposition modeling (FDM) technique and to evaluate their physicochemical and biological properties. Structural, chemical, mechanical, and biological characterizations were performed. We successfully fabricated scaffolds of three different pore sizes. However, the pore dimensions were slightly smaller than expected. We found that the 3D printing process induced decreases in both, PLA molecular weight and degradation temperatures, but did not change the semicrystalline structure of the polymer. We did not observe any effect of pore size on the mechanical properties of produced scaffolds. After the sterilization by γ irradiation, scaffolds did not exhibit any cytotoxicity towards human bone marrow stromal cells (HBMSC). Finally, after three and seven days of culture, HBMSC showed high viability and homogenous distribution irrespective of pore size. Thus, these results suggest that FDM technology is a fast and reproducible technique that can be used to fabricate tridimensional custom-made scaffolds for tissue engineering. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 887-894, 2018.

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Nanoengineered Endocytic Biomaterials for Stem Cell Therapy

Wang,

Sun,

Liu

et al. 2024

Adv Funct Materials

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Stem cells, ideal for the tissue repair and regeneration, possess extraordinary capabilities of multidirectional differentiation and self‐renewal. However, the limited spontaneous differentiation potential makes it challenging to harness them for tissue repair without external intervention. Although conventional approaches using biomolecules, small organic molecules, and ions have shown specific and effective functions, they face challenges such as in vivo diffusion and degradation, poor internalization, and side effects on adjacent cells. Nanoengineered biomaterials offer a solution by solidifying and nanosizing these soluble regulating molecules and ions, facilitating their uptake by stem cells. Once inside lysosomes, these nanoparticles release their contents in a controlled “molecule or ion storm,” efficiently altering the intracellular biological and chemical microenvironment to tune the differentiation of stem cells. This newly emerged approach for regulating stem cell fate has attracted much attention in recent years. This method has shown promising results and is poised to enhance clinical stem cell therapy. This review provides an overview of the design principles for nanoengineered biomaterials, discusses the categories and characteristics of nanoparticles, summarizes the application of nanoparticles in tissue repair and regeneration, and discusses the direction of nanoparticle‐enhanced stem cell therapy and prospects for its clinical application in regenerative medicine.

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Layer-by-layer bioassembly of cellularized polylactic acid porous membranes for bone tissue engineering

Cited by 39 publications

References 43 publications

Layer‐by‐layer bioassembly of poly(lactic) acid membranes loaded with coculture of HBMSCs and EPCs improves vascularization in vivo

Layer‐by‐layer bioassembly of poly(lactic) acid membranes loaded with coculture of HBMSCs and EPCs improves vascularization in vivo

Characterization of printed PLA scaffolds for bone tissue engineering

Nanoengineered Endocytic Biomaterials for Stem Cell Therapy

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