1998
DOI: 10.1021/ac970536b
|View full text |Cite
|
Sign up to set email alerts
|

Layer-by-Layer Construction of Enzyme Multilayers on an Electrode for the Preparation of Glucose and Lactate Sensors:  Elimination of Ascorbate Interference by Means of an Ascorbate Oxidase Multilayer

Abstract: A layer-by-layer structure of enzyme multilayers composed of glucose oxidase (GOx) or lactate oxidase (LOx) and ascorbate oxidase (AOx) was prepared on the surface of a platinum electrode. The amperometric response to glucose or lactate was studied in the presence of ascorbic acid as a possible interference. An alternating and repeated deposition of avidin and the biotin-labeled enzymes resulted in the layer-by-layer structure of GOx/AOx and LOx/AOx multilayers. Optical and gravimetric measurements based on an… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

1
97
0
3

Year Published

1998
1998
2012
2012

Publication Types

Select...
8

Relationship

2
6

Authors

Journals

citations
Cited by 170 publications
(101 citation statements)
references
References 33 publications
1
97
0
3
Order By: Relevance
“…Such high enzyme loadings of glucose oxidase could be achieved with SAM enzyme electrodes if a number of single molecule thick enzyme layers are deposited by exploiting some of the recently developed step-by-step immobilization techniques. [3][4][5][6][7][8][9][10][11][12][13][14] Such an approach could then lead to a transport limited enzyme electrode being fabricated in a highly controlled manner, thus exploiting the highly controlled immobilization virtues of the SAM approach with the advantages of transport limitation.…”
Section: What Limits the Response Of The Sam Enzyme Electrode?mentioning
confidence: 99%
See 1 more Smart Citation
“…Such high enzyme loadings of glucose oxidase could be achieved with SAM enzyme electrodes if a number of single molecule thick enzyme layers are deposited by exploiting some of the recently developed step-by-step immobilization techniques. [3][4][5][6][7][8][9][10][11][12][13][14] Such an approach could then lead to a transport limited enzyme electrode being fabricated in a highly controlled manner, thus exploiting the highly controlled immobilization virtues of the SAM approach with the advantages of transport limitation.…”
Section: What Limits the Response Of The Sam Enzyme Electrode?mentioning
confidence: 99%
“…Using SAMs has the potential to provide enzyme electrodes with a high degree of reproducibility, 1,2 molecular level control over the spatial distribution of the immobilized enzymes [3][4][5][6][7][8][9][10][11][12][13][14] and the immobilization of the enzyme close to the electrode thus allowing direct electron transfer to be achieved. [15][16][17][18][19][20][21][22][23][24] These advantages have resulted in a recent surge in research into self-assembled monolayers for biosensor applications in general, and enzyme electrodes in particular.…”
mentioning
confidence: 99%
“…4 A selective permeable membrane immobilized with an enzyme on an electrode surface is available for the elimination of interfering substances. [5][6][7] However, in some cases, the selective permeable membrane cannot eliminate the interfering substances completely. In addition, most amperometric enzyme biosensor tips are disposable.…”
Section: Introductionmentioning
confidence: 99%
“…In this context, we have demonstrated that enzyme multilayer membranes can be prepared by an alternate deposition of avidin and biotin-labeled enzymes on the surface of a quartz slide and an electrode ( Fig. 1) [4][5][6][7][8]. The enzyme multilayer membranes thus prepared rely on the strong affinity between avidin and biotin [9] and are characterized by an alternate deposition of monomolecular layers of avidin and enzyme.…”
Section: Introductionmentioning
confidence: 99%