Layer-by-Layer Deposition of Hyaluronan and Quercetin-Loaded Chitosan Nanoparticles onto Titanium for Improving Blood Compatibility

Abstract

Surface modification is an effective way to improve the hemocompatibility of biomaterials. Quercetin has significant anticoagulation and antithrombotic effects, and thus it is a promising candidate agent for the surface modification of blood-contacting materials. In this study, quercetin was successfully encapsulated in tripolyphosphate–chitosan nanoparticles (TCs) based on the ionic gelation of chitosan with tripolyphosphate (TPP) anions. Then, hyaluronan acid (HA)/quercetin-loaded TPP–chitosan nanoparticle (QTCs) films, in addition to HA/TCs films, were prepared separately using an electrostatic layer-by-layer self-assembly technique. The encapsulation of quercetin in the chitosan nanoparticles was confirmed by UV spectra. The quercetin-loaded multilayer coatings were also successfully self-assembled, as confirmed by the UV spectra and contact angle measurements. Platelet adhesion experiments were carried out with platelet-enriched plasma so as to evaluate the blood compatibility of the different samples. There were many platelets on the surfaces of the glass and HA/TC-coated titanium, which were partially activated but not aggregated. Meanwhile, many more platelets were observed on the uncoated titanium surfaces, most of which developed pseudopodia. By contrast, the platelet adhesion and activation were reduced remarkably on the surface of the HA/QTC-coated titanium. These results showed that the multilayer coatings containing quercetin could act as potential biomaterials to improve the anticoagulation performance of blood-contacting materials.

Chitosan-Based Anti-Oxidation Delivery Nano-Platform: Applications in the Encapsulation of DHA-Enriched Fish Oil

“…CS NPs have been used for loading insulin and also applied in diabetes therapy [ 28 ]. Moreover, several food bioactive ingredients have been encapsulated in CS NPs, including curcumin [ 29 ], flavonoids [ 30 ], lutein [ 31 ], polyphenols [ 32 ], resveratrol [ 33 ], and vitamins (B9, B12, and C) [ 34 ]. Fish oils are rich in ω-3 PUFAs, which are highly prone to oxidation due to their higher content of unsaturated fatty acid undergoing lipid oxidation.…”

Loading Gentamicin and Zn2+ on TiO2 Nanotubes to Improve Anticoagulation, Endothelial Cell Growth, and Antibacterial Activities

“…Although great progress has been made in the surface functionalization of titanium-based biomaterials, there are still many issues to be solved, including the delayed surface endothelialization, thrombosis, and infection after implantation [ 12 , 13 ]. Studies have shown that the surface properties of the implant are related not only to the surface bioactivities but also to the surface topographies.…”

Development of an electrospun polycaprolactone/silk scaffold for potential vascular tissue engineering applications

“…This detection limit was expressed by Hemo-R. Hemo-R should not be more than 5%, indicating that the material could meet the biomedical requirements. 51,52 In this study, the direct method was that various proportions of the microfiber scaffold and collected fresh rabbit blood were co-incubated for 3 h, while distilled water and saline solution were used as positive and negative controls, respectively. As previous research had proven that pure SF has excellent blood compatibility, we did not test the pure SF experimental group.…”