Layer-by-Layer Deposition with Polymers Containing Nitrilotriacetate, A Convenient Route to Fabricate Metal- and Protein-Binding Films

Wijeratne, Salinda; Liu, Weijing; Dong, Jinlan; Ning, Wenjing; Ratnayake, Nishanka Dilini; Walker, Kevin D.; Bruening, Merlin L.

doi:10.1021/acsami.6b00896

Cited by 21 publications

(14 citation statements)

References 50 publications

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“…It has been widely used for: biomedical applications [3], corrosion control [3], catalysis [4], drug delivery [5], electrochemical sensing [6], protein purification [7], electrochromism [8], ultrastrong materials [9], optics and optoelectronics [10], among others [11][12][13]. …”

Section: Introductionmentioning

confidence: 99%

Simultaneous characterization of physical, chemical, and thermal properties of polymeric multilayers using infrared spectroscopic ellipsometry

Castilla‐Casadiego

Pinzón-Herrera

Pérez‐Pérez

et al. 2018

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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In this study, multilayered films of polyethylenimine/poly (sodium-p-styrene sulfonate) (PEI)/(PSS) and type I collagen/heparin sodium (COL)/(HEP) were fabricated using the layer-by-layer technique, and fully characterized using Infrared Variable Angle Spectroscopic Ellipsometry (IRVASE) to simultaneously analyze the chemistry, thickness, and roughness of the multilayers with respect to changes in pH of the washing solution, and changes in temperature. Film topography and Young’s modulus were obtained by atomic force microscopy (AFM) and nanoindentation. Our results show that with IRVASE it is possible to analyze the thickness of the multilayers prepared using a washing solution of pH 5, obtaining values of 71.7 nm and 40.3 nm for three bilayers of PEI/PSS and COL/HEP, respectively. Film roughness varies between multilayer systems, obtaining values of 37.76 nm for three bilayers of PEI/PSS and 33.58 nm for three bilayers of COL/HEP. Increasing the pH of the washing solution for PEI/PSS yielded thinner films that were less susceptible to thermal induced changes in film chemistry in the range of 25 – 150 °C. PEI/PSS films decreased in thickness with increasing temperature up to 75 °C, whereas above 75 °C film thickness increased. Through IRVASE, a transition temperature for the PEI/PSS multilayers was observed at 75 °C. Temperatures above 37 °C drastically alter the chemistry and the thickness of the COL/HEP multilayers indicating a possible degradation of the polymers. We obtained, through nanoindentation, a Young’s modulus of 15000 kPa and 9000 kPa for 12 bilayers of PEI/PSS and COL/HEP, respectively. These results demonstrate that, using IRVASE, we can simultaneously evaluate the physical, chemical, and thermal properties of synthetic and natural multilayered polymeric films.

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Section: Introductionmentioning

confidence: 99%

Simultaneous characterization of physical, chemical, and thermal properties of polymeric multilayers using infrared spectroscopic ellipsometry

Castilla‐Casadiego

Pinzón-Herrera

Pérez‐Pérez

et al. 2018

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…Distinct building blocks and strategies have been extensively investigated to enable a reliable, functional healing as some polymers involved in the process are vulnerable to abrasion. Again, the LbL technique is as a powerful tool in the buildup of multifunctional interfaces based on spontaneous non-covalent interactions of materials, tuning the interface characteristics [62][63][64]. Such interactions introduce properties not exhibited by individual materials, which can be easily exemplified with polyethylenimine (PEI) and poly(acrylic acid) (PAA).…”

Section: Multifunctional Materialsmentioning

confidence: 99%

Multilayered Nanostructures Integrated with Emerging Technologies

Braunger¹,

Hensel²,

Gaál³

et al. 2020

Multilayer Thin Films - Versatile Applications for Materials Engineering

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Surface and interface functionalization are crucial steps to introduce new functionalities in numerous applications, as faster dynamics occur on surfaces rather than bulk. Within this context, the layer-by-layer (LbL) technique is a versatile methodology to controllably form organized nanostructures from the spontaneous adsorption of charged molecules. It enables the assembly of multilayered LbL films on virtually any surface using non-covalent molecular interactions, allowing the nanoengineering of interfaces and creation of multifunctional systems with distinct building blocks (polymers, clays, metal nanoparticles, enzymes, organic macromolecules, etc.). Several applications require thin films on electrodes for sensing/ biosensing, and here we explore LbL films deposited on interdigitated electrodes (IDEs) that were 3D-printed using the fusing deposition modeling (FDM) technique. IDEs covered with LbL films can be used to form multisensory systems employed in the analysis of complex liquids transforming raw data into specific patterns easily recognized by computational and statistical methods. We extend the FDM 3D-printing methodology to simplify the manufacturing of electrodes and microchannels, thus integrating an e-tongue system in a microfluidic device. Moreover, the continuous flow within microchannels contributes to faster and more accurate analysis, reducing the amount of sample, waste, and costs.

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“… 43 − 47 Adsorption of a poly(acrylic acid) (PAA)-containing film in membrane pores followed by activation of the film enables immobilization of affinity groups at high densities. 42 , 43 , 48 − 50 Modification of pores with either polymer brushes 45 − 47 or layer-by-layer films 42 , 43 , 51 can create affinity membranes with high binding capacities, and membrane modification using layer-by-layer deposition of a few polyelectrolyte layers is very convenient.…”

Section: Introductionmentioning

confidence: 99%

Quantitation of Trastuzumab and an Antibody to SARS-CoV-2 in Minutes Using Affinity Membranes in 96-Well Plates

et al. 2022

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Quantitation of therapeutic monoclonal antibodies (mAbs) in human serum could ensure that patients have adequate levels of mAbs for effective treatment. This research describes the use of affinity, glass-fiber membranes in a 96-well-plate format for rapid (<5 min) quantitation of the therapeutic mAb trastuzumab and a mAb against the SARS-CoV-2 spike protein. Adsorption of a poly(acrylic acid)-containing film in membrane pores and activation of the −COOH groups in the film enable covalent-linking of affinity peptides or proteins to the membrane. Passage of mAb-containing serum through the affinity membrane results in mAb capture within 1 min. Subsequent rinsing, binding of a secondary antibody conjugated to a fluorophore, and a second rinse yield mAb-concentration-dependent fluorescence intensities in the wells. Calibration curves established from analyses on different days have low variability and allow determination of mAb levels in separately prepared samples with an average error <10%, although errors in single-replicate measurements may reach 40%. The assays can occur in diluted serum with physiologically relevant mAb concentrations, as well as in undiluted serum. Thus, the combination of 96-well plates containing affinity membranes, a microplate reader, and a simple vacuum manifold affords convenient mAb quantitation in <5 min.

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Layer-by-Layer Deposition with Polymers Containing Nitrilotriacetate, A Convenient Route to Fabricate Metal- and Protein-Binding Films

Cited by 21 publications

References 50 publications

Simultaneous characterization of physical, chemical, and thermal properties of polymeric multilayers using infrared spectroscopic ellipsometry

Simultaneous characterization of physical, chemical, and thermal properties of polymeric multilayers using infrared spectroscopic ellipsometry

Multilayered Nanostructures Integrated with Emerging Technologies

Quantitation of Trastuzumab and an Antibody to SARS-CoV-2 in Minutes Using Affinity Membranes in 96-Well Plates

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