2013

DOI: 10.1166/sl.2013.2729

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Layer-by-Layer Films Containing SiO<SUB>2</SUB> Nanoparticles as Field-Effect pH Sensors

Izabela G. Arruda¹,

Nirton Cristi Silva Vieira²,

et al.

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“…A pH sensitivity of (29 ± 1) mV.pH −1 was obtained ( Fig. 2b ), which is in agreement with the literature 14 15 .…”

Section: Resultssupporting

confidence: 92%

Electrical Detection of Dengue Biomarker Using Egg Yolk Immunoglobulin as the Biological Recognition Element

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,

²

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³

et al. 2015

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Nonstructural protein 1 (NS1) is secreted by dengue virus in the first days of infection and acts as an excellent dengue biomarker. Here, the direct electrical detection of NS1 from dengue type 2 virus has been achieved by the measurement of variations in open circuit potential (OCP) between a reference electrode and a disposable Au electrode containing immobilized anti-NS1 antibodies acting as immunosensor. Egg yolk immunoglobulin (IgY) was utilized for the first time as the biological recognition element alternatively to conventional mammalian antibodies in the detection of dengue virus NS1 protein. NS1 protein was detected in standard samples in a 0.1 to 10 µg.mL−1 concentration range with (3.2 ± 0.3) mV/µg.mL−1 of sensitivity and 0.09 µg.mL−1 of detection limit. Therefore, the proposed system can be extended to detect NS1 in real samples and provide an early diagnosis of dengue.

“…A pH sensitivity of (29 ± 1) mV.pH −1 was obtained ( Fig. 2b ), which is in agreement with the literature 14 15 .…”

Section: Resultssupporting

confidence: 92%

Electrical Detection of Dengue Biomarker Using Egg Yolk Immunoglobulin as the Biological Recognition Element

¹

,

²

,

³

et al. 2015

Self Cite

View full text Add to dashboard Cite

Nonstructural protein 1 (NS1) is secreted by dengue virus in the first days of infection and acts as an excellent dengue biomarker. Here, the direct electrical detection of NS1 from dengue type 2 virus has been achieved by the measurement of variations in open circuit potential (OCP) between a reference electrode and a disposable Au electrode containing immobilized anti-NS1 antibodies acting as immunosensor. Egg yolk immunoglobulin (IgY) was utilized for the first time as the biological recognition element alternatively to conventional mammalian antibodies in the detection of dengue virus NS1 protein. NS1 protein was detected in standard samples in a 0.1 to 10 µg.mL−1 concentration range with (3.2 ± 0.3) mV/µg.mL−1 of sensitivity and 0.09 µg.mL−1 of detection limit. Therefore, the proposed system can be extended to detect NS1 in real samples and provide an early diagnosis of dengue.

Potentiometric detection of chemical species by spin-assisted assembly of vanadium pentoxide nanorods

¹

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²

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³

et al. 2016

Sensors and Actuators B: Chemical

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Label-free electrical recognition of a dengue virus protein using the SEGFET simplified measurement system

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²

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³

et al. 2014

Anal. Methods

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Separative extended gate field-effect transistor was applied as an immunosensor for the label-free recognition of dengue virus nonstructural protein 1 (NS1).

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