Layer‐by‐layer inorganic/polymeric nanoparticles for kinetically controlled multigene delivery

Bishop, Corey J.; Liu, Allen L.; Lee, David S.; Murdock, Richard J.; Green, Jordan J.

doi:10.1002/jbm.a.35610

Cited by 23 publications

(18 citation statements)

References 27 publications

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“…Furthermore, the obtained lipopolymers could be used in the context of other techniques intended to control and improve multigene delivery as with the layer-by-layer technique reported by Bishop et al . (2016) [ 80 ]. Moreover, it would be very interesting to evaluate the performance of these kinds of cationic lipopolymers to interact and protect dsRNA, siRNA or other negatively charged molecules intended to be delivered in vivo .…”

Section: Conclusion Perspectives and Future Applicationsmentioning

confidence: 99%

Diacetylenic lipids in the design of stable lipopolymers able to complex and protect plasmid DNA

et al. 2017

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Different viral and non-viral vectors have been designed to allow the delivery of nucleic acids in gene therapy. In general, non-viral vectors have been associated with increased safety for in vivo use; however, issues regarding their efficacy, toxicity and stability continue to drive further research. Thus, the aim of this study was to evaluate the potential use of the polymerizable diacetylenic lipid 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DC8,9PC) as a strategy to formulate stable cationic lipopolymers in the delivery and protection of plasmid DNA. Cationic lipopolymers were prepared following two different methodologies by using DC8,9PC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and the cationic lipids (CL) 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), stearylamine (SA), and myristoylcholine chloride (MCL), in a molar ratio of 1:1:0.2 (DMPC:DC8,9PC:CL). The copolymerization methodology allowed obtaining cationic lipopolymers which were smaller in size than those obtained by the cationic addition methodology although both techniques presented high size stability over a 166-day incubation period at 4°C. Cationic lipopolymers containing DOTAP or MCL were more efficient in complexing DNA than those containing SA. Moreover, lipopolymers containing DOTAP were found to form highly stable complexes with DNA, able to resist serum DNAses degradation. Furthermore, neither of the cationic lipopolymers (with or without DNA) induced red blood cell hemolysis, although metabolic activity determined on the L-929 and Vero cell lines was found to be dependent on the cell line, the formulation and the presence of DNA. The high stability and DNA protection capacity as well as the reduced toxicity determined for the cationic lipopolymer containing DOTAP highlight the potential advantage of using lipopolymers when designing novel non-viral carrier systems for use in in vivo gene therapy. Thus, this work represents the first steps toward developing a cationic lipopolymer-based gene delivery system using polymerizable and cationic lipids.

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Section: Conclusion Perspectives and Future Applicationsmentioning

confidence: 99%

Diacetylenic lipids in the design of stable lipopolymers able to complex and protect plasmid DNA

et al. 2017

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“…Utilizing polymers or dendrimers instead of lipids allows inorganic nanoparticles to be encased in a shell to create core/shell nanosystems (Figure D–F); , inversely, polymer nanoparticles can also be functionalized with surface-associated inorganic components (Figure G) . The layer-by-layer architecture involves sequential coating of metallic nanoparticles by polymer and therapeutic to create nanosystems with tunable release profiles (Figure H) . Finally, the rattle-type architecture consists of a solid core nanoparticle surrounded by an unattached porous shell (Figure I) .…”

Section: Organic Building Blocksmentioning

confidence: 99%

Hybrid Nanosystems for Biomedical Applications

et al. 2021

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Inorganic/organic hybrid nanosystems have been increasingly developed for their versatility and efficacy at overcoming obstacles not readily surmounted by nonhybridized counterparts. Currently, hybrid nanosystems are implemented for gene therapy, drug delivery, and phototherapy in addition to tissue regeneration, vaccines, antibacterials, biomolecule detection, imaging probes, and theranostics. Though diverse, these nanosystems can be classified according to foundational inorganic/organic components, accessory moieties, and architecture of hybridization. Within this Review, we begin by providing a historical context for the development of biomedical hybrid nanosystems before describing the properties, synthesis, and characterization of their component building blocks. Afterward, we introduce the architectures of hybridization and highlight recent biomedical nanosystem developments by area of application, emphasizing hybrids of distinctive utility and innovation. Finally, we draw attention to ongoing clinical trials before recapping our discussion of hybrid nanosystems and providing a perspective on the future of the field.

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“…Release of the nucleic acid is dependent on the degradability of polymers within the coating and their electrostatic interactions and can occur over a period of hours or days depending on the composition of the layers [104]. Two nucleic acids can also be loaded and released sequentially from LbLcoated nanoparticles, leading to the expression of two genes on different timescales [105]. LbL techniques utilizing PBAE have also been employed to generate 3D surfaces for gene and protein delivery.…”

Section: Pbae-based Hybrid Materialsmentioning

confidence: 99%

Poly(beta-amino ester)s as gene delivery vehicles: challenges and opportunities

Karlsson

Rhodes

Green

et al. 2020

Expert Opinion on Drug Delivery

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Introduction: Gene delivery technologies are being developed for an increasing number of biomedical applications, with delivery vehicles including viruses and non-viral materials. Among biomaterials used for non-viral gene delivery, poly(beta-amino ester)s (PBAEs), a class of synthetic, biodegradable polymers, have risen as a leading gene delivery vehicle that has been used for multiple applications in vitro and in vivo.Areas covered: This review summarizes the key properties of PBAEs and their development, including a discussion of the advantages and disadvantages of PBAEs for gene delivery applications. The use of PBAEs to improve the properties of other drug delivery vehicles is also summarized. Expert opinion:PBAEs are designed to have multiple characteristics that are ideal for gene delivery, including their reversible positive charge, which promotes binding to nucleic acids as well as imparting high buffering capacity, and their rapid degradability under mild conditions. Simultaneously, some of their properties also lead to nanoparticle instability and low transfection efficiency in physiological environments. The ease with which PBAEs can be chemically modified

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Layer‐by‐layer inorganic/polymeric nanoparticles for kinetically controlled multigene delivery

Cited by 23 publications

References 27 publications

Diacetylenic lipids in the design of stable lipopolymers able to complex and protect plasmid DNA

Diacetylenic lipids in the design of stable lipopolymers able to complex and protect plasmid DNA

Hybrid Nanosystems for Biomedical Applications

Poly(beta-amino ester)s as gene delivery vehicles: challenges and opportunities

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