Layer-by-Layer Investigation of Ultrastructures and Biomechanics of Human Cornea

Rates, Erick Rafael Dias; Almeida, Charles Duarte; Costa, Eduardo de Freitas; Farias, Roberta Jansen de Mello; Santos-Oliveira, Ralph; Alencar, Luciana Magalhães Rebêlo

doi:10.3390/ijms23147833

Cited by 10 publications

(4 citation statements)

References 45 publications

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“…Tissue shape and mechanics, for example, are critical for regulating organ function. AFM can capture high-resolution images and connect them with mechanical and viscoelastic properties, making it an ideal tool for studying blood cells, cancer cells, , and viral particles, among other biological systems . It is worth noting that AFM can be utilized in the physiological circumstances of cells or biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Atomic Force Microscopy Applied to the Study of Tauopathies

do Nascimento Amorim,

Silva França,

Santos-Oliveira

et al. 2024

ACS Chem. Neurosci.

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Atomic force microscopy (AFM) is a scanning probe microscopy technique which has a physical principle, the measurement of interatomic forces between a very thin tip and the surface of a sample, allowing the obtaining of quantitative data at the nanoscale, contributing to the surface study and mechanical characterization. Due to its great versatility, AFM has been used to investigate the structural and nanomechanical properties of several inorganic and biological materials, including neurons affected by tauopathies. Tauopathies are neurodegenerative diseases featured by aggregation of phosphorylated tau protein inside neurons, leading to functional loss and progressive neurotoxicity. In the broad universe of neurodegenerative diseases, tauopathies comprise the most prevalent, with Alzheimer's disease as its main representative. This review highlights the use of AFM as a suitable research technique for the study of cellular damages in tauopathies, even in early stages, allowing elucidation of pathogenic mechanisms of these diseases.

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Section: Introductionmentioning

confidence: 99%

Atomic Force Microscopy Applied to the Study of Tauopathies

do Nascimento Amorim,

Silva França,

Santos-Oliveira

et al. 2024

ACS Chem. Neurosci.

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“…During the separation process, the zero deflection of the cantilever beam also does not mean that the probe is separated from the sample. Due to the adhesive forces between the probe tip and the sample, the cantilever beam deforms and forces in the opposite direction before the two are truly separated (Dias Rates et al, 2022). The “instantaneous” separation occurs when the probe continues to move away from the sample and overcomes the adhesive forces (Holuigue et al, 2022).…”

Section: Basic Introduction Of Afmmentioning

confidence: 99%

Atomic force microscopy in disease‐related studies: Exploring tissue and cell mechanics

Liu,

Han,

Kong

et al. 2023

Microscopy Res & Technique

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Despite significant progress in human medicine, certain diseases remain challenging to promptly diagnose and treat. Hence, the imperative lies in the development of more exhaustive criteria and tools. Tissue and cellular mechanics exhibit distinctive traits in both normal and pathological states, suggesting that “force” represents a promising and distinctive target for disease diagnosis and treatment. Atomic force microscopy (AFM) holds great promise as a prospective clinical medical device due to its capability to concurrently assess surface morphology and mechanical characteristics of biological specimens within a physiological setting. This review presents a comprehensive examination of the operational principles of AFM and diverse mechanical models, focusing on its applications in investigating tissue and cellular mechanics associated with prevalent diseases. The findings from these studies lay a solid groundwork for potential clinical implementations of AFM.Research HighlightsBy examining the surface morphology and assessing tissue and cellular mechanics of biological specimens in a physiological setting, AFM shows promise as a clinical device to diagnose and treat challenging diseases.

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“…The lucidity of the cornea is due to the complicated balance between the layers and the cellular components of the cornea. 1 Cornea is an immune-privileged structure, which provides protection to the eye and is the reason for successful corneal transplants. 1,2 Corneal neovascularization occurs in various pathologies and can result in compromise of corneal transparency.…”

Section: Introductionmentioning

confidence: 99%

“…1 Cornea is an immune-privileged structure, which provides protection to the eye and is the reason for successful corneal transplants. 1,2 Corneal neovascularization occurs in various pathologies and can result in compromise of corneal transparency. This results in impaired visual acuity, corneal edema and scarring.…”

Section: Introductionmentioning

confidence: 99%

Effect of Intra-Stromal Bevacizumab on Corneal Vascularization

Niazi¹,

Masood²,

Ali³

et al. 2022

pak J Ophthalmol

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Purpose: To study the effect of Intra-stromal Bevacizumab on corneal vascularization. Study Design: Quasi experimental study. Place and Duration of Study: Outpatient department of the Combined Military Hospital, Multan from April 2022 to August 2022. Methods: Thirty patients were recruited through non-probability, convenience sampling technique. Ethical approval was taken from Institutional Review Board. Complete ocular examination was performed by a consultant ophthalmologist and patients with corneal neovascularization were included in the study. Area of corneal vascularization was measured on slit lamp. After taking all the aseptic measures and administering anesthetic eye drops, patients were administered 0.2 ml of (2.5 mg/0.1 mL) Bevacizumab (Avastin, Roche) using an ophthalmic microscope. The patient was then called for a follow up after 4 weeks and then the grade of corneal vascularization was checked by a consultant ophthalmologist on slit lamp examination. Data analysis was doneusing SPSS version 25. Results: Mean age of the patients was 43.4 ± 13.7 years (range 25 to 72 years). Male to female ratio was 2:3. There was significant decrease in the area of corneal neovascularization from 2.78mm ± 0.30 to 2.50mm ± 0.27 (p = 0.0003). The mean change in the area of corneal neovascularization was 10.07%. Two patients had sub-conjunctival hemorrhage which settled over due course of time. Conclusion: Intra-stromal Bevacizumab resulted in significant reduction in vascularized area of cornea with minimal side effects indicating that intra-stromal Bevacizumab is an effective and safe modality in the treatment of corneal neovascularization.

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Layer-by-Layer Investigation of Ultrastructures and Biomechanics of Human Cornea

Cited by 10 publications

References 45 publications

Atomic Force Microscopy Applied to the Study of Tauopathies

Atomic Force Microscopy Applied to the Study of Tauopathies

Atomic force microscopy in disease‐related studies: Exploring tissue and cell mechanics

Effect of Intra-Stromal Bevacizumab on Corneal Vascularization

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