Layer-specific cell differentiation in bi-layered vascular grafts under flow perfusion

Abstract

DOI to the publisher's website.• The final author version and the galley proof are versions of the publication after peer review.• The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publication General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal.If the publication is distributed under the terms of Article 25fa of the Dutch Copyright Act, indicated by the "Taverne" license above, please follow below link for the End User Agreement:

Melt Electrospinning of Nanofibers from Medical‐Grade Poly(ε‐Caprolactone) with a Modified Nozzle

“…[ 30 ] While sharp edges in electrohydrodynamic have been adopted especially for SES scale up, [ 22,31 ] the acupuncture needle approach adopted here both introduces a sharp point and limits the fluid flow through the nozzle. With this approach, high quality MES substrates could be made that have applicability for biomedical [ 32,33 ] and filtration applications. [ 34 ]…”

“…[ 34 ] Using the system here, a similar purpose product can be made without requiring solvent use. Similarly, solution electrospun tubes that are melt electrowritten upon for vascular approaches [ 32 ] could be made solvent‐free without compromising the “barrier effect” that the small diameter electrospun fibers are intended to provide. Broadly speaking, such modified needles have generic utility in biomedical materials to make small diameter nanofibers while not requiring the use of solvents.…”

“…While current manufacturing throughput for MES is notably low in this study, the placement of the electrospun fibers on a specific spot on the collector is highly predictable and the head small enough to be employed within a hybrid manufacturing approach (shown with MEW as an example in this study) for biomaterial applications where defined barriers are of interest. [ 32–34 ]…”

Cellular Geometry Sensing at Different Length Scales and its Implications for Scaffold Design

“…For example, neuron growth is strongly guided by the directions of scaffold fibers, leading to various approaches to induce nerve repair by tuning the properties of biomaterials and scaffolds [41,42]. Another example is in the case of (micro)vascular tissue engineering, where multiple cell types need to organize in different configurations (e.g., endothelial cells in a concave tubular construct, wrapped around by pericytes) to ensure function within the microvasculature [43,44]. Moreover, increasing attention has been focused toward the question of how cells actually perceive these geometrical cues of the extracellular environment, in addition to the downstream effect of the cue on the cellular behavior.…”

Topographic Guidance in Melt-Electrowritten Tubular Scaffolds Enhances Engineered Kidney Tubule Performance

“…Twisting scaffolds would additionally protect and stabilize the constructs. To engineer peritubular blood vessels, the initial pore size could be reduced by combination of MEW with electrospinning, as shown by Jungst et al and Pennings et al[42,43]. Furthermore, the use of supportive cell types like pericytes or fibroblasts could help in the formation of endothelial monolayers[44].…”