Layered Double Hydroxide Nanoparticles for Efficient Gene Delivery for Cancer Treatment

Senapati, Sudipta; Sarkar, Tanmoy; Das, Parimal; Maiti, Pralay

doi:10.1021/acs.bioconjchem.9b00434

Cited by 30 publications

(15 citation statements)

References 45 publications

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“…The solid-state UV–vis spectra of pure PLA, DNA, and different DNA-incorporated PLA–DNA complexes have been shown in Figure b. Pristine DNA shows a broad absorption peak at around 285 nm associated with heterocyclic ring structure of the DNA bases . After incorporation of DNA into the polymer matrix, a blue shift of DNA peak is observed at 275 nm, presumably due to the strong dipolar interactions between DNA and the PLA molecules.…”

Section: Results and Discussionmentioning

confidence: 84%

“…(a) Polarized optical microscopy (POM) investigation of PLA and PLA–DNA samples; (b) solid-state comparative UV–vis spectra of blank PLA, naked DNA, and PLA–DNA; (c) reflectance mode FTIR spectra of the samples; and (d) DSC thermograms of different samples showing different melting behaviors of DNA in the presence of PLA and in naked state. UV−vis, FTIR, and DSC data for DNA samples were obtained from our previous work …”

Section: Results and Discussionmentioning

confidence: 99%

“…Although viral vectors possess high transfection properties, safety-related concerns limit their use as gene delivery systems, and thereby nonviral gene delivery vectors are a more attractive candidate for gene delivery. , Cationic, amine-containing polymers, such as polyethylenimine (PEI), have been tried widely as an attractive alternative to viral gene delivery systems due to their high transfection efficiency. However, considerable cytotoxicity with high-molecular-weight PEI has been reported in various studies. , Biodegradable polymers − and inorganic , nanoparticles have been getting considerable attention in recent times for safe and efficient gene delivery.…”

Section: Introductionmentioning

confidence: 99%

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Controlled DNA Delivery Using Poly(lactide) Nanoparticles and Understanding the Binding Interactions

et al. 2021

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Cationic polymer-based gene delivery vectors suffer from several limitations such as low DNA-loading capacity, poor transfection, toxicity, environmental degradations, etc. Again, very limited works are available addressing the binding interactions in detail at the atomic level explaining the loading capacity, protection ability against harsh environments, and controlled release behavior of the DNA-encapsulated vehicles. Here, a poly(L-lactide) (PLA) nanoparticle-based controlled DNA release system is proposed. The developed vehicle possesses a high DNA-loading capacity and can release the loaded DNA in a controlled manner. Spectroscopic, physicochemical, and molecular simulation techniques (AM1 and atomistic molecular dynamics) have been employed to understand the binding interactions between PLA and DNA molecules enabling high DNA loading, protection against external harsh environments, and controlled DNA release behavior. Methyl thiazolyl tetrazolium (MTT) assay experiments confirm the biocompatible nature of the vehicle. Cellular uptake efficiency and endo-lysosomal escape capabilities have been investigated against HeLA cells. This study, therefore, demonstrates the development of a promising nonviral DNA delivery vector and includes a detailed investigation of the atomic-level interaction behavior between PLA and DNA molecules.

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Section: Results and Discussionmentioning

confidence: 84%

Section: Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Controlled DNA Delivery Using Poly(lactide) Nanoparticles and Understanding the Binding Interactions

et al. 2021

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“…Due to the remarkable development in nanotechnology, a considerable number of excellent works have been devoted to constructing nontoxic delivery systems with the basic concepts of low toxicity and high transfection efficiency. Nonviral vehicles include cationic lipids (Lipoplexes) [ 15 ], cationic polymers (polyplexes) [ 16 , 17 ], organic nanoparticles [ 18 ], and inorganic nanoparticles [ 19 ]. For instance, polyethyleneimine (PEI) [ 20 ], poly-lysine (PLL), poly-amidoamine (PAMAM) [ 21 ], lipofectamine [ 22 ], and graphene quantum dots [ 23 ] are employed for systemic administration by mimicking the functions of viral cell entry that enable stronger binding affinity and avoiding the immune potential and toxicity risks of viral vectors [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Topography-Mediated Enhancement of Nonviral Gene Delivery in Stem Cells

Yang

Bron

et al. 2022

Pharmaceutics

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Gene delivery holds great promise for bioengineering, biomedical applications, biosensors, diagnoses, and gene therapy. In particular, the influence of topography on gene delivery is considered to be an attractive approach due to low toxicity and localized delivery properties. Even though many gene vectors and transfection systems have been developed to enhance transfection potential and combining it with other forms of stimulations could even further enhance it. Topography is an interesting surface property that has been shown to stimulate differentiation, migration, cell morphology, and cell mechanics. Therefore, it is envisioned that topography might also be able to stimulate transfection. In this study, we tested the hypothesis “topography is able to regulate transfection efficiency”, for which we used nano- and microwave-like topographical substrates with wavelengths ranging from 500 nm to 25 µm and assessed the transfectability of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) and myoblasts. For transfection, Lipofectamine 2000 and a gene encoding plasmid for red-fluorescent protein (m-Cherry) were used and topography-induced cell morphology and transfection efficiency was analyzed. As a result, topography directs cell spreading, elongation, and proliferation as well as the transfection efficiency, which were investigated but were found not to be correlated and dependent on the cell type. A 55% percent improvement of transfection efficiency was identified for hBM-MSCs grown on 2 µm wrinkles (24.3%) as compared to hBM-MSCs cultured on flat controls (15.7%). For myoblast cells, the highest gene-expression efficiency (46.1%) was observed on the 10 µm topography, which enhanced the transfection efficiency by 64% as compared to the flat control (28.1%). From a qualitative assessment, it was observed that the uptake capacity of cationic complexes of TAMRA-labeled oligodeoxynucleotides (ODNs) was not topography-dependent but that the intracellular release was faster, as indicated by the positively stained nuclei on 2 μm for hBM-MSCs and 10 μm for myoblasts. The presented results indicate that topography enhances the gene-delivery capacity and that the responses are dependent on cell type. This study demonstrates the important role of topography on cell stimulation for gene delivery as well as understanding the uptake capacity of lipoplexes and may be useful for developing advanced nonviral gene delivery strategies.

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“…LDHs have also been largely reported for biological and other broad applications [ [17] , [18] , [19] , [20] , [21] ]. Most studies have focused on the development of nanosystem formulations for cancer treatment [ 10 , 22 , 23 ], inflammatory [ 7 , 15 , 24 ], cardiovascular and the fight against Acquired Immunodeficiency Syndrome virus (AIDS) [ [25] , [26] , [27] ]. LDHs can be described by the general formula

[ 28 , 29 ], where

and

are respectively divalent and trivalent cations,

represents the interlayer charge of the ionic molecule,

is the molar fraction of the trivalent cation.…”

Section: Introductionmentioning

confidence: 99%