Layered Double Hydroxides/Trypsin Based Conductometric Biosensors

Mansouri, Hela; Forano, Claude; Prévot, Vanessa; Jaffrézic‐Renault, Nicole; Amara, Abdesslem Ben Haj

doi:10.1166/sl.2009.1167

Cited by 7 publications

(3 citation statements)

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“…Various soft chemical routes such as adsorption, delamination/restacking, layer by layer self-assembly (LbL), coprecipitation and electrogeneration have been used to immobilize different oxido-reductases, urease or trypsine in LDH host structure for amperometric [63] and conductimetric [62,64] biosensor applications. Such enzymes immobilization onto LDHs preserves their native integrity [62,63].…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

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LDHs as Electrode Materials for Electrochemical Detection and Energy Storage: Supercapacitor, Battery and (Bio)-Sensor

Mousty

Leroux²

2012

NANOTEC

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From an exhaustive overview based on applicative academic literature and patent domain, the relevance of Layered Double Hydroxide (LDHs) as electrode materials for electrochemical detection of organic molecules having environmental or health impact and energy storage is evaluated. Specifically the focus is driven on their application as supercapacitor, alkaline or lithium battery and (bio)-sensor. Inherent to the high versatility of their chemical composition, charge density, anion exchange capability, LDH-based materials are extensively studied and their performances for such applications are reported. Indeed the analytical characteristics (sensitivity and detection limit) of LDH-based electrodes are scrutinized, and their specific capacity or capacitance as electrode battery or supercapacitor materials, are detailed.

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Section: Electrochemical Biosensorsmentioning

confidence: 99%

“…This enzyme was immobilized within ZnAl and MgAl LDHs by coprecipitation and used for the conductimetric detection of N -benzoyl-L-Arginine ethyl ester, as a model substrate [64].…”

Section: Other Electrochemical Biosensorsmentioning

confidence: 99%

LDHs as Electrode Materials for Electrochemical Detection and Energy Storage: Supercapacitor, Battery and (Bio)-Sensor

Mousty

Leroux²

2012

NANOTEC

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“…Layered Double Hydroxides (LDHs) are well known in immobilizing biologically active materials such as porphyrins (Park et al, 1989;Bonnet et al, 1996;Robbins and Dutta, 1996), nucleoside phosphates (Choy et al, 2000;Choy et al, 2001;Minagawa et al, 2019), drugs Fardella et al, 1997 b ; Fardella et al, 1998;Fogg et al, 1998;Khan et al, 2001;Choi et al, 2018), vitamins (Hwang et al, 2001), amino acids (Whilton et al, 1997;Aisawa et al, 2000;Aisawa et al, 2001) and fatty acids (Meyn et al, 1990;Borja and Dutta, 1992). It is believed that the host lattice may protect these relatively delicate, but comparatively large biomolecules from degradation and also aid their transport to specific targets within the body (Mansouri et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Investigation of enhancement in thermal stability of trypsin in modified Mg/Al layered double hydroxides

et al. 2021

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Mg/Al Layered Double Hydroxides (LDHs) were functionalized to immobilize the serine protease, trypsin. Three methods were implemented for immobilization: physical adsorption, entrapment and covalent cross-linking. Trypsin was immobilized in Mg/Al-NO 3-LDH via simple adsorption. The same LDH host was modified with Sodium Dodecyl Sulphate (SDS) which assembles inside the double layer, for the enzyme to be entrapped. For covalent cross linking, LDH host was modified with vertical pillars of glutamate ions which were cross-linked to horizontally aligned dicarbonyl linkers. This cross linkage firmly holds the enzyme via Schiff's base linkages using the free amino groups of the enzyme. Thermal stability and storage stability of the immobilized enzyme was studied in comparison with the free enzyme using trypsin activity assay experiments. The enzyme showed remarkable stability against autolysis even at higher temperatures showing the potential of modified LDHs to store trypsin at room temperature.

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Hybrid and biohybrid layered double hydroxides for electrochemical analysis

Mousty

Prévot

2013

Anal Bioanal Chem

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Layered double hydroxides (LDH) are lamellar materials that have been extensively used as electrode modifiers. Nanostructured organic-inorganic materials can be designed by intercalation of organic or metallic complexes within the interlayer space of these materials or by the formation of composite materials based on biopolymers (alginate or chitosan) or biomolecules, such as enzymes. These hybrid or biohybrid materials have interesting properties applicable in electroanalytical devices. From an exhaustive review of the literature, the relevance of these hybrid and biohybrid LDH materials as electrode materials for electrochemical detection of species with an environmental or health impact is evaluated. The analytical characteristics (sensitivity and detection limit) of LDH-based amperometric sensors or biosensors are scrutinized.

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Layered Double Hydroxides/Trypsin Based Conductometric Biosensors

Cited by 7 publications

References 0 publications

LDHs as Electrode Materials for Electrochemical Detection and Energy Storage: Supercapacitor, Battery and (Bio)-Sensor

LDHs as Electrode Materials for Electrochemical Detection and Energy Storage: Supercapacitor, Battery and (Bio)-Sensor

Investigation of enhancement in thermal stability of trypsin in modified Mg/Al layered double hydroxides

Hybrid and biohybrid layered double hydroxides for electrochemical analysis

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