Layering of different materials to achieve optimal conditions for treatment of painful wounds

Maver, Tina; Gradišnik, Lidija; Kurečič, Manja; Hribernik, Silvo; Smrke, Dragica; Maver, Uroš; Stana‐Kleinschek, Karin

doi:10.1016/j.ijpharm.2017.07.043

Cited by 37 publications

(37 citation statements)

References 61 publications

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“…Thus, the release exponent values in the range 0.18–0.43 reflect a non-Fickian indomethacin transport mechanism from the designed supports, indicating that several physical-chemical processes are involved in the drug release, as previously reported by us and also by other authors [19,23,29,30]: (1) an initial desorption of the drug retained at the sponge surface; (2) absorption of the release medium by the hydrophilic biopolymeric sponges followed by their swelling; (3) progressive transformation of spongious matrix in gel; (4) diffusion of the drug immobilized in the collagen fibrillar/PVA structure concomitantly with (5) gradual erosion of the release support.…”

Section: Discussionsupporting

confidence: 83%

Collagen-Polyvinyl Alcohol-Indomethacin Biohybrid Matrices as Wound Dressings

et al. 2018

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The aim of this study is to design, develop and evaluate new biohybrid sponges based on polymers (collagen and polyvinyl alcohol) with and without indomethacin as anti-inflammatory drug model to be used for tissue regeneration in wound healing. Type I fibrillar collagen in the form of a gel and different concentrations of polyvinyl alcohol were mixed together to prepare composite gels. Both control samples, without indomethacin and with indomethacin, were obtained. All samples were crosslinked with glutaraldehyde. By freeze-drying of hydrogels, the spongious forms (matrices) were obtained. The matrices were characterized by FT-IR spectroscopy, scanning electron microscopy (SEM), water absorption, enzymatic degradation and in vitro indomethacin release. The pharmacological effect of the spongious biohybrid matrices was determined on an experimental model of burns induced to Wistar rats. The SEM images showed a porous structure with interconnected pores. Collagen sponges present a structure with pore sizes between 20 and 200 µm, which became more and more compact with polyvinyl alcohol addition. The FT-IR showed interactions between collagen and polyvinyl alcohol. The enzymatic degradation indicated that the most stable matrix is the one with the ratio 75:25 of collagen:polyvinyl alcohol (ACI75), the other ones being degradable in time. The kinetic data of indomethacin release from matrices were fitted with different kinetic models and highlighted a biphasic release of the drug. Such kinetic profiles are targeted in skin wound healing for which important aspects are impaired inflammation and local pain. The treatment with sponges associated with anti-inflammatory drug had beneficial effects on the healing process in experimentally induced burns compared to the corresponding matrices without indomethacin and the classical treated control group.

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Section: Discussionsupporting

confidence: 83%

Collagen-Polyvinyl Alcohol-Indomethacin Biohybrid Matrices as Wound Dressings

et al. 2018

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“…This is a commonly used and reliable method to evaluate cell proliferation (Finšgar et al, 2016; Maver et al, 2018). It is based on the reduction of the yellow MTT by metabolically active cells (e.g., in part by dehydrogenase enzymes), which results in the formation of nicotinamide adenine dinucleotide and/or nicotinamide adenine dinucleotide phosphate (Maver et al, 2017). During this reaction, a purple formazan is formed intracellularly, which can be solubilized and quantified by spectrophotometric means.…”

Section: Methodsmentioning

confidence: 99%

Polysaccharide Thin Solid Films for Analgesic Drug Delivery and Growth of Human Skin Cells

et al. 2019

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Chronic wounds not only lower the quality of patient's life significantly, but also present a huge financial burden for the healthcare systems around the world. Treatment of larger wounds often requires the use of more complex materials, which can ensure a successful renewal or replacement of damaged or destroyed tissues. Despite a range of advanced wound dressings that can facilitate wound healing, there are still no clinically used dressings for effective local pain management. Herein, alginate (ALG) and carboxymethyl cellulose (CMC), two of the most commonly used materials in the field of chronic wound care, and combination of ALG-CMC were used to create a model wound dressing system in the form of multi-layered thin solid films using the spin-assisted layer-by-layer (LBL) coating technique. The latter multi-layer system was used to incorporate and study the release kinetics of analgesic drugs such as diclofenac and lidocaine at physiological conditions. The wettability, morphology, physicochemical and surface properties of the coated films were evaluated using different surface sensitive analytical tools. The influence of in situ incorporated drug molecules on the surface properties (e.g., roughness) and on the proliferation of human skin cells (keratinocytes and skin fibroblasts) was further evaluated. The results obtained from this preliminary study should be considered as the basis for the development “real” wound dressing materials and for 3D bio-printing applications.

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“…The formation of cell aggregates further indicates an increased cell proliferation and the establishment of intercellular communication, which plays a vital role in the inhibition of apoptosis (indirectly observed among others through the absence of red-dyed dead cells after 29 days of culturing) [60]. It is well known that mammalian cells are less prone to intensively proliferate in contact with ALG-based materials (despite the ALGs proven biocompatibility with various cells [61]) due to the hydrophilic nature of alginic acid, which adsorbs less protein, leading to reduced attachment and spreading, as well as due to an "unstable surface", which results from potential ion exchange (Na + and Ca 2+ ; as described above). In this regard, several engineering approaches have been investigated to improve cell adhesion on alginate-based substrates.…”

Section: Live/dead Assay To Evaluate the Viability Of The Cell-laden mentioning

confidence: 99%

Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

et al. 2020

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Limitations in wound management have prompted scientists to introduce bioprinting techniques for creating constructs that can address clinical problems. The bioprinting approach is renowned for its ability to spatially control the three-dimensional (3D) placement of cells, molecules, and biomaterials. These features provide new possibilities to enhance homology to native skin and improve functional outcomes. However, for the clinical value, the development of hydrogel bioink with refined printability and bioactive properties is needed. In this study, we combined the outstanding viscoelastic behavior of nanofibrillated cellulose (NFC) with the fast cross-linking ability of alginate (ALG), carboxymethyl cellulose (CMC), and encapsulated human-derived skin fibroblasts (hSF) to create a bioink for the 3D bioprinting of a dermis layer. The shear thinning behavior of hSF-laden bioink enables construction of 3D scaffolds with high cell density and homogeneous cell distribution. The obtained results demonstrated that hSF-laden bioink supports cellular activity of hSF (up to 29 days) while offering proper printability in a biologically relevant 3D environment, making it a promising tool for skin tissue engineering and drug testing applications.

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Layering of different materials to achieve optimal conditions for treatment of painful wounds

Cited by 37 publications

References 61 publications

Collagen-Polyvinyl Alcohol-Indomethacin Biohybrid Matrices as Wound Dressings

Collagen-Polyvinyl Alcohol-Indomethacin Biohybrid Matrices as Wound Dressings

Polysaccharide Thin Solid Films for Analgesic Drug Delivery and Growth of Human Skin Cells

Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis

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