“…In the case of R-004, however, it was found that the CMC or CAC determined by the surface tension method may not accurately represent the concentration at which aggregation starts. The formation of sub-micelles has also been reported in casein [Koczo et al, 1996], SC3 hydrophobin [Corvis et al, 2006] and low molecular weight versus such as SDS [Cui et al, 2008]. In these cases, the CMC obtained from the adsorption isotherm may correspond to saturation of the interface.…”