Layers of regulation – Insights into the role of transcription factors controlling mucilage production in the Arabidopsis seed coat

Golz, John F.; Allen, Patrick J; Li, Song F.; Parish, Roger W.; Jayawardana, Nadeeka Upamali; Bacic, Antony; Doblin, Monika S.

doi:10.1016/j.plantsci.2018.04.021

Cited by 56 publications

(71 citation statements)

References 123 publications

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“…In the model land plant Arabidopsis thaliana, specialised mucilage secretory cells (MSCs) in the outer integument produce large amounts of mucilaginous hydrophilic polysaccharides in rings around a central columella that is compositionally similar to a secondary cell wall (Haughn & Western, 2012). The mucilaginous rings in each cell dehydrate during seed maturation but rapidly expand when in contact with water during imbibition of the mature seed, accompanied by rupturing and rapid degradation of the outermost cell walls (Golz et al, 2018). The released mucilage envelope can be divided into an inner and an outer layer (Haughn & Western, 2012), and this organization provides coherence and stability (Voiniciuc et al, 2015).…”

Section: Desiccation Prevention and Surface Adhesion Properties Of Sementioning

confidence: 99%

“…Highthroughput screens for phenotypic differences in the mucilaginous seed coats of Arabidopsis mutants have helped to identify specific genes that secure proper seed coat production (Golz et al, 2018, and references herein). In particular, these studies have highlighted the contribution of transcription factors to MSC differentiation and have led to a proposed hierarchical network involving three distinct tiers or levels of regulation (Golz et al, 2018). Seed coats are a potential source for carbon and therefore tentatively a source for nourishing the developing seed, although some studies suggest that this resource is not utilised (Huang et al, 2004).…”

Section: Desiccation Prevention and Surface Adhesion Properties Of Sementioning

confidence: 99%

“…Some molecules within mucilages have bioadhesive properties, making them sought-after materials for biotechnological, biomedical or agricultural applications (Favi et al, 2014;George & Suchitra, 2019). Important breakthroughs have been made in the last few years in investigations of the sticky adhesives used by English ivy and by sundew (Zhang et al, 2010;Lenaghan & Zhang, 2012), and a current focus on molecular genetic investigations of seed coats (Golz et al, 2018;Sechet et al, 2018) and the rhizosheath (Sasse et al, 2018) underpins the potential of this field. With this review we first highlight the wealth of the different sticky mucilage types that are produced by plants.…”

Section: Introductionmentioning

confidence: 99%

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Sticky mucilages and exudates of plants: putative microenvironmental design elements with biotechnological value

2019

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Summary Plants produce a wide array of secretions both above and below ground. Known as mucilages or exudates, they are secreted by seeds, roots, leaves and stems and fulfil a variety of functions including adhesion, protection, nutrient acquisition and infection. Mucilages are generally polysaccharide‐rich and often occur in the form of viscoelastic gels and in many cases have adhesive properties. In some cases, progress is being made in understanding the structure–function relationships of mucilages such as for the secretions that allow growing ivy to attach to substrates and the biosynthesis and secretion of the mucilage compounds of the Arabidopsis seed coat. Work is just beginning towards understanding root mucilage and the proposed adhesive polymers involved in the formation of rhizosheaths at root surfaces and for the secretions involved in host plant infection by parasitic plants. In this article, we summarise knowledge on plant exudates and mucilages within the concept of their functions in microenvironmental design, focusing in particular on their bioadhesive functions and the molecules responsible for them. We draw attention to areas of future knowledge need, including the microstructure of mucilages and their compositional and regulatory dynamics.

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Section: Desiccation Prevention and Surface Adhesion Properties Of Sementioning

confidence: 99%

Section: Desiccation Prevention and Surface Adhesion Properties Of Sementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sticky mucilages and exudates of plants: putative microenvironmental design elements with biotechnological value

2019

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“…The seed coat epidermal differentiation starts after fertilization and proceeds until the mature green stage, corresponding to 0~13 DPA (Francoz et al, 2015; Golz et al, 2018). The ERF4 expression is gradually increased during this differentiation phase, and peaks at 13 DPA (Figure 1A).…”

Section: Discussionmentioning

confidence: 99%

“…The Arabidopsis seed mucilage has been shown to be composed of two layers, termed the water-soluble outer layer and the adherent inner layer (Western et al, 2001). Structural analysis reveals that both layers are composed primarily of pectic polysaccharide rhamnogalacturonan I (RG-I), whereas homogalacturonan (HG) and RG-II are minor components (Golz et al, 2018; Macquet et al, 2007). In the inner layer, cellulose and hemicellulose can also be found, contributing to the tight attachment of the adherent layer to the seed coat, whereas the soluble outer layer is easily lost (Griffiths et al, 2016; Sullivan et al, 2011; Willats et al, 2001a).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis ERF4 and MYB52 Transcription Factors Play Antagonistic Roles in Regulating Homogalacturonan De-methylesterification in Seed Coat Mucilage

Ding

Tang

Han

et al. 2020

Preprint

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One-sentence summary: Arabidopsis ERF4 and MYB52 transcription factors interact 18 and play antagonistic roles in regulating homogalacturonan de-methylesterification 19 related genes in the seed coat mucilage.ABSTRACT 24 The Arabidopsis (Arabidopsis thaliana) seed coat mucilage is a specialized cell wall with 25 pectin as its major component. Pectin is synthesized in the Golgi apparatus with 26 homogalacturonan fully methylesterified, but it must undergo de-methylesterification by 27 pectin methylesterase (PME) after being secreted into the cell wall. This reaction is 28 critical for pectin maturation, but the mechanisms of its transcriptional regulation remain 29 largely unknown. Here, we show that the Arabidopsis ERF4 transcription factor 30 positively regulates pectin de-methylesterification during seed development and directly 31 suppresses the expression of PME INHIBITOR13 (PMEI13), 14, 15 and 32 SUBTILISIN-LIKE SERINE PROTEASE 1.7 (SBT1.7). The erf4 mutant seeds showed 33 repartitioning of mucilage between soluble and adherent layers as a result of decreased 34 PME activity and increased degree of pectin methylesterification. ERF4 physically 35 associates with and antagonizes MYB52 in activating PMEI6, 14 and SBT1.7 and 36 MYB52 also antagonizes ERF4 activity in the regulation of downstream targets. Gene 37 expression studies revealed that ERF4 and MYB52 have opposite effects on pectin 38 de-methylesterification. Genetic analysis indicated that the erf4-2 myb52 double mutant 39 seeds show mucilage phenotype similar to wild-type. Taken together, this study 40 demonstrates that ERF4 and MYB52 antagonize each other's activity to maintain the 41 appropriate degree of pectin methylesterification, expanding our understanding of how 42 pectin de-methylesterification is fine-tuned by the ERF4-MYB52 transcriptional complex 43 in the seed mucilage. 44 130 6 stress (Liu et al., 2017). ERF4 is expressed ubiquitously in Arabidopsis (Winter et al., 131 2007), suggesting that it has other functions in addition to those mentioned above. For 132 example, its regulatory role in cell wall biology has not been reported yet. 133Here, we provide evidence that ERF4 positively regulates pectin de-methylesterification 134 in the seed mucilage. We find a repartitioning of seed mucilage polysaccharides in the 135 erf4 seeds under vigorous shaking conditions which can be attributed to an increase in 136 DM. ERF4 binds to the cis-regulatory elements and directly suppresses the expression of 137 PMEI13, 14, 15 and SBT1.7. Moreover, ERF4 and MYB52 were found to interact, and 138 play completely opposite roles in pectin de-methylesterification by antagonizing each 139 other's transcriptional activity, which is confirmed by gene expression and genetic 140 evidence. Overall, these findings provide a fine-tuned mechanism where ERF4 and 141 MYB52 antagonistically control mucilage modification related genes. 142 RESULTS 143Expression pattern of ERF4 is correlated with seed mucilage deposition 144 Previous studies revea...

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Arabidopsis Seed Mucilage: A Specialised Extracellular Matrix that Demonstrates the Structure–Function Versatility of Cell Wall Polysaccharides

Šola

Dean

Haughn

2019

Annual Plant Reviews Online

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Arabidopsis seed coat mucilage is a specialised extracellular matrix composed of the same broad classes of polysaccharides found in a primary cell wall (cellulose, pectin, and hemicellulose), arranged in a distinct structure with special properties. It is deposited by the seed coat epidermal cells in the apoplast in a polar manner to produce a doughnut‐shaped mucilage pocket on the outside of the cell. Following exposure to water the mucilage extrudes from the outer surface of the cell. A portion of this mucilage remains strongly adherent to the seed. The unique features of mucilage make it amenable to study, and genetic, cytological, and biochemical analyses have been used to determine its composition, synthesis, and structure, and establish how these combine to determine its properties. Lessons learned from these studies strengthen and extend our understanding of plant extracellular matrices, especially the cell wall. In this article, we summarise and contextualise what is currently known about mucilage by focusing on its main features – extrusion and adherence.

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Layers of regulation – Insights into the role of transcription factors controlling mucilage production in the Arabidopsis seed coat

Cited by 56 publications

References 123 publications

Sticky mucilages and exudates of plants: putative microenvironmental design elements with biotechnological value

Sticky mucilages and exudates of plants: putative microenvironmental design elements with biotechnological value

Arabidopsis ERF4 and MYB52 Transcription Factors Play Antagonistic Roles in Regulating Homogalacturonan De-methylesterification in Seed Coat Mucilage

Arabidopsis Seed Mucilage: A Specialised Extracellular Matrix that Demonstrates the Structure–Function Versatility of Cell Wall Polysaccharides

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