LbAMT3-1, an ammonium transporter induced by arbuscular mycorrhizal in Lycium barbarum, confers tobacco with higher mycorrhizal levels and nutrient uptake

Cheng, Kang; Wei, Man; Jin, Xinqiao; Tang, Ming; Zhang, Haoqiang

doi:10.1007/s00299-022-02847-0

Cited by 8 publications

(9 citation statements)

References 10 publications

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“…The results showed that CE only worked well in the regulation of MDA and soluble protein, while R. irregularis and mixtures played good effects on four indicators. Studies have shown that some species in Rhizophagus could induce the specific expression of transporters and improve plant growth under adversity [51], besides, AM fungi played roles by community in the field, some species would appear together to stabilize the community composition but performed minor biological functions [52], moreover, there was almost no vesicle in the roots colonized with CE, suggesting that CE was underdeveloped in V. prionantha [53], which would affect the functions. These were likely the reasons for the better effects of RI and CE + RI.…”

Section: Discussionmentioning

confidence: 99%

Effects of Different Arbuscular Mycorrhizal Fungi on Physiology of Viola prionantha under Salt Stress

Liu¹,

Fang²,

Zhao³

et al. 2023

Phyton

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Arbuscular mycorrhizal (AM) fungi distribute widely in natural habits and play a variety of ecological functions. In order to test the physiological response to salt stress mediated by different AM fungi, Viola prionantha was selected as the host, the dominant AM fungus in the rhizosphere of V. philippica growing in Songnen saline-alkali grassland, Rhizophagus irregularis, and their mixtures were used as inoculants, and NaCl stress was applied after the roots were colonized. The results showed that V. philippica could be colonized by AM fungi in the field and the colonization rate ranged from 73.33% to 96.67%, and Claroideoglomus etunicatum was identified as the dominant AM fungi species in the rhizosphere of V. philippica by morphology combined with sequencing for AM fungal AML1/AML2 target. Inoculation with both the species resulted in the formation of mycorrhizal symbiosis (the colonization rate was more than 70%) and AM fungi significantly enhanced plants' tolerance to salt stress of varying magnitude. Higher activity of antioxidant enzymes and augmented levels of proline and other osmoregulators were observed in AM plants. The content of MDA in CK was higher than that in the inoculations with the stress of 100, 200, and 250 mM. All indices except soluble protein content and MDA content were significantly correlated with AM fungal colonization indices. The analysis for different AM fungal effects showed that the mixtures and R. irregularis worked even better than C. etunicatum. These results will provide theoretical support for the exploration and screening of salt-tolerant AM fungi species and also for the application of AM-ornamental plants in saline-alkali urban greening.

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Section: Discussionmentioning

confidence: 99%

Effects of Different Arbuscular Mycorrhizal Fungi on Physiology of Viola prionantha under Salt Stress

Liu¹,

Fang²,

Zhao³

et al. 2023

Phyton

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“…Beyond AtAMT1;1 , the other Arabidopsis AMTs have also restored 31019b growth under low-ammonium conditions [ 4 ]. In recent years, the 31019b yeast mutant has been widely applied for functional complementation studies of homologous AMT genes in several plant species [ 39 , 40 , 41 , 42 ]. For example, in the present study, four of the five rapeseed AMT proteins selected for testing proved capable of restoring 31019b growth under low-ammonium conditions, with BnaAMT2;2a being the lone exception ( Figure 8 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of Ammonium Transporter (AMT) Genes in Rapeseed (Brassica napus L.)

Dai

Han²,

Walk

et al. 2023

Genes

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Ammonium transporters (AMTs) are plasma membrane proteins mediating ammonium uptake and transport. As such, AMTs play vital roles in ammonium acquisition and mobilization, plant growth and development, and stress and pathogen defense responses. Identification of favorable AMT genotypes is a prime target for crop improvement. However, to date, systematic identification and expression analysis of AMT gene family members has not yet been reported for rapeseed (Brassica napus L.). In this study, 20 AMT genes were identified in a comprehensive search of the B. napus genome, 14 members of AMT1 and 6 members of AMT2. Tissue expression analyses revealed that the 14 AMT genes were primarily expressed in vegetative organs, suggesting that different BnaAMT genes might function in specific tissues at the different development stages. Meanwhile, qRT-PCR analysis found that several BnaAMTs strongly respond to the exogenous N conditions, implying the functional roles of AMT genes in ammonium absorption in rapeseed. Moreover, the rapeseed AMT genes were found to be differentially regulated by N, P, and K deficiency, indicating that crosstalk might exist in response to different stresses. Additionally, the subcellular localization of several BnaAMT proteins was confirmed in Arabidopsis protoplasts, and their functions were studied in detail by heterologous expression in yeast. In summary, our studies revealed the potential roles of BnaAMT genes in N acquisition or transportation and abiotic stress response and could provide valuable resources for revealing the functionality of AMTs in rapeseed.

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“…Rhizophagus irregularis Blaszk., Wubet, Renker & Buscot (BGC BJ109) was provided by the Plant Nutrition and Resources Institutes of Beijing Academy of Agriculture and Forestry. Rhizophagus irregularis was the dominant AM fungal species in the rhizosphere of Goji in Ningxia province in China, so this AM fungal strain was used in a previous study [22]. Plantago asiatica L., which was cultivated using autoclaved sands, was used as the host plant for R. irregularis propagation for 12 months.…”

Section: Biological Materials and Growth Substratementioning

confidence: 99%

“…Real-time quantitative PCR (qPCR) was performed on a CF96X Real-Time PCR System (Bio-Rad, Hercules, CA, USA) to analyze the transcript accumulation of LbAMT3−1. The reaction volume was 10 µL, which contained 0.5 µL of each gene-specific primer (10 µM), 1 µL of cDNA, 3 µL of RNase-free H 2 O, and 5 µL of ChamQ SYBR qPCR Master Mix (Vazyme, Nanjing, China) [22].…”

Section: Quantitative Real-time Pcr Analysismentioning

confidence: 99%

“…However, the influence of AM fungi on the performance of L. barbarum with different N (forms and concentrations) fertilizers is not fully understood. The response of a recently isolated NH 4 + transporter (LbAMT3-1) [15,22], which may regulate NH 4 + transport from AM fungi to L. barbarum, is also unclear. Therefore, a pot experiment was conducted in this study with the following objectives: (1) to evaluate the effect of AM fungal colonization on the growth performance (biomass accumulation, N accumulation, photosynthesis, and root viability) of L. barbarum and (2) to compare the response of LbAMT3-1 expression to different N supplies.…”

Section: Introductionmentioning

confidence: 99%

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Symbiosis of Arbuscular Mycorrhizal Fungi and Lycium barbarum L. Prefers NO3− over NH4+

et al. 2023

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Nitrogen (N) is an essential nutrient that plants require and is, most of the time, limited in different terrestrial ecosystems. Forming symbioses with plants, arbuscular mycorrhizal (AM) fungi improve mineral element uptake and the net primary production of plants. Recent reports have suggested that AM fungi mediate N uptake in plants. However, there are fewer studies on the influence of AM fungi on the response of Lycium barbarum, a medicinal plant in northwest China, under different N-addition conditions. In this study, the effect of Rhizophagus irregularis, N forms (NO3− and NH4+), and N levels (1.5, 7.5, 15, 30 mM) on the performance of L. barbarum was evaluated through a pot experiment. The application of R. irregularis significantly improved L. barbarum biomass, net photosynthetic rate, and root tissue viability under adequate NO3− and NH4+ supplies, and mycorrhizal plants showed better performance under NO3− supply. AM colonization enhanced N acquisition under adequate NO3− supply and strongly induced the expression of LbAMT3-1 in L. barbarum roots. Based on these results, we propose that NO3−-dominated N supply favors mycorrhizal symbiosis to a greater extent than NH4+; this study provides a basis for maintaining beneficial AM symbiosis during nitrogen fertilizer use in arable land.

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LbAMT3-1, an ammonium transporter induced by arbuscular mycorrhizal in Lycium barbarum, confers tobacco with higher mycorrhizal levels and nutrient uptake

Cited by 8 publications

References 10 publications

Effects of Different Arbuscular Mycorrhizal Fungi on Physiology of Viola prionantha under Salt Stress

Effects of Different Arbuscular Mycorrhizal Fungi on Physiology of Viola prionantha under Salt Stress

Genome-Wide Identification and Characterization of Ammonium Transporter (AMT) Genes in Rapeseed (Brassica napus L.)

Symbiosis of Arbuscular Mycorrhizal Fungi and Lycium barbarum L. Prefers NO3− over NH4+

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