LC Determination and Stability Assessment of Macrolide Antibiotics Azithromycin and Spiramycin in Bulk and Tablet Samples

Mahmoudi, Abdelghani

doi:10.18052/www.scipress.com/ilns.47.1

Cited by 2 publications

(1 citation statement)

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“…The sample preparation was done based on an already published protocol . An amount equivalent to 450 mg of spiramycin was transferred to 200 mL volumetric flask with 50 mL water/acetonitrile (7:3, v/v) and shaken for 20 min, followed by marking up to volume with the same solvent.…”

Section: Methodsmentioning

confidence: 99%

Efficient and simple HPLC method for spiramycin determination in urine samples and in pharmaceutical tablets

Mahmoudi

2018

Separation Science Plus

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High‐performance liquid chromatography was used in the development of a new simple method for the determination of spiramycin in urine and tablets. Experimental and instrumental conditions were first optimized for the separation of this compound and then the method was applied to real samples. The procedure was performed using an isocratic elution on ODB reversed phase C18 column (250 × 4.6 mm, 5 μm) with UV detection at 231 nm, and a very reasonable resolution was achieved. In urine, the developed method was employed after a simple and fast sample preparation involving pre‐column for clean‐up procedure, and using roxithromycin as internal standard. Linearity, in the range of 0.3–25 μg mL−1 showed a good linear correlation (R2 = 0.9994), with a detection limit down to 30 ng mL−1. In the following step, the quantification of spiramycin was studied in pharmaceutical forms. Results showed a good repeatability and the relative standard deviation was below to 2.18%. Precision was checked at three levels during three consecutive days with intra‐day and inter‐day coefficients of relative standard deviation not exceeding the value of 4.98%. The extraction recovery presented good results with values ranging from 90.12–101.13% for both matrixes.

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Section: Methodsmentioning

confidence: 99%