LC-ESI-MS Analysis, Antitumor and Antiviral activities of Bosica senegalensis aqueous methanolic extract

Elkhateeb, Ahmed; Hussein, Sameh R.; Salem, Maha; Negoumy, Sabry El

doi:10.21608/ejchem.2018.4828.1428

Cited by 4 publications

(7 citation statements)

References 14 publications

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“…(2015) breast, lung, skin B. rufescens Garbi et al. (2015) skin B. senegalensis Elkhateeb et al. (2019) breast, colon C. longa Li et al., 2018 , Li et al., 2018 , Li et al., 2018 ; Coker-Gurkan et al.…”

Section: Resultsmentioning

confidence: 99%

Integration of medicinal plants into the traditional system of medicine for the treatment of cancer in Sokoto State, Nigeria

Malami

Jagaba

Abubakar

et al. 2020

Heliyon

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This study was designed to explore and record various medicinal plants integrated into the traditional system of medicine for the treatment of cancer. The traditional system of medicine is a routine practiced among the indigenous ethnic groups of Sokoto state. A semi-structured questionnaire was designed and used for data collection around the selected Local Government Areas. A substantial number of plant species were identified, recorded, and collected for preservation. Data collected for each specie was analysed to assess its frequent use among the medicinal plants. A total of 67 species belonging to 31 families have been identified and recorded. Out of the 473 frequency of citation (FC), Acacia nilotica was the most frequently cited specie (32 FC, 64% FC, 0.6 RFC), followed by Guiera senegalensis (27 FC, 54% FC, 0.5 RFC), Erythrina sigmoidea (17 FC, 34% FC, 0.3 RFC), and subsequently Combretum camporum (15 FC, 30% FC, 0.3 RFC). The most common parts of the plants used include the barks (55.2%), the roots (53.2%), and the leaves (41.8%). Additionally, decoction (74.6%), powdered form (49.3%), and maceration (46.3%) are the most frequently used mode of preparation. The historical knowledge of a traditional system of medicine practiced by the native traditional healers of Sokoto for the treatment of cancer has been documented. The present study further provides a baseline for future pharmacological investigations into the beneficial effects of such medicinal plants for the treatment of cancer.

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Section: Resultsmentioning

confidence: 99%

Integration of medicinal plants into the traditional system of medicine for the treatment of cancer in Sokoto State, Nigeria

Malami

Jagaba

Abubakar

et al. 2020

Heliyon

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“…1. Among them, four flavonoids were unambiguously identified as quercetin 3,4′-diglucoside (2), isoquercitrin (7), quercetin (16), and kaempferol (17) by comparing their retention time (Rt) with those of standard compounds (Fig. 1S, Supporting Information), while the structure of kaempferol-3-O-glucoside (9) was confirmed after preparative isolation.…”

Section: Resultsmentioning

confidence: 98%

“…In the MS 3 spectrum, the base peak at m/z 207 was diagnostic of the presence of sinapoyl residue, and the fragment ion [M + H – 162 – 162 – 302] + suggested the loss of hexose and aglycone moieties ( Fig. 13S and 14S , Supporting Information) 17 , 18 . Both compounds had similar UV and MS 2 spectra, suggesting them to be quercetin- O -sinapoyl hexoside- O -hexoside isomers.…”

Section: Resultsmentioning

confidence: 99%

“…A stock solution of 1 mg/mL containing four standards, i.e., quercetin 3,4′-diglucoside (2), isoquercitrin (7), quercetin (16), and kaempferol (17), was prepared in methanol. Serial dilutions were prepared in methanol to establish calibration curves.…”

Section: Preparation Of Stock Solution and Calibration Curvesmentioning

confidence: 99%

“…In the present study, LC-MS was used to identify seventeen flavonoids in the active fraction (Oxy50-60F) of O. esculentum. Among them, quercetin 3,4'-diglucoside (2), isoquercitrin (7), kaempferol-3-O-glucoside (9), quercetin (16), and kaempferol (17) were unambiguously identified and quantified by comparison with those authentic standards or after isolation. The bone anabolic activity of the purified flavonoid enriched fraction (Oxy50-60F) was tested by measuring the alkaline phosphatase enzyme, an important osteoblast differentiation marker which provides a high concentration of phosphate during bone minerlisation.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Bone Fracture-healing Properties and UPLC-MS Analysis of an Enriched Flavonoid Fraction from Oxystelma esculentum

Rawat,

Sharma,

Khanka

et al. 2023

Planta Med

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Oxystelma esculentum has been used as a folk medicine to treat jaundice, throat infections, and skin problems. In the current study, the bone fracture-healing properties of a flavonoid enriched fraction (Oxy50-60F) of O. esculentum were investigated in Swiss mice using a drill-hole injury model. Oxy50-60F (1 mg/kg/day, 5 mg/kg/day, and 10 mg/kg/day) was administered orally (from the next day) after a 0.6 mm drill-hole injury in mice femur mid diaphysis for 7 days and 14 days. Parathyroid hormone (40 μg/kg; 5 times/week) was given subcutaneously as the positive control. Confocal imaging for bone regeneration, micro-architecture of femur bones, ex-vivo mineralisation, haematoxyline & eosin staining, measurement of reactive oxygen species, gene expression of osteogenic and anti-inflammatory genes were studied. Quercetin, kaempferol, and isorhamnetin glycosides were identified in the active fraction using mass spectrometry techniques. Our results confirm that Oxy50-60F treatment promotes fracture healing, callus formation at drill-hole sites and stimulates osteogenic and anti-inflammatory genes. Oxy50-60F administration to fractured mice exhibited significantly better micro-CT parameters in a dose-dependent manner and promoted nodule mineralisation at days 7 and 14 post-injury. Oxy50-60F also prevents ROS generation by increasing expression of the SOD2 enzyme. Overall, this study reveals that Oxy50-60F has bone regeneration potential in a cortical bone defect model, which supports its use in delayed-union and non-union fracture cases.

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UPLC-ESI-MS polyphenolic profiling and the antimicrobial potential of Ailanthus altissima (Mill.) Swingle leaves growing in Egypt

Raafat MOHAMED,

WAKIL,

HASHASH

et al. 2024

jrp

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The need of finding out new and potent antimicrobial agents to combat the multidrug resistance pathogens becomes critical. Ailanthus altissima is characterized by its vast and remarkable therapeutic potential as a traditional folk medicine. The current study aims to investigate the polyphenolic profiling and the antimicrobial activity of the methanol extract of A. altissima leaves and its derived fractions. The antimicrobial activity was investigated through two in-vitro assays; agar well diffusion and broth microdilution against six pathogens while the polyphenolic profiling was characterized through UPLC-ESI-MS analysis. All the test pathogens were susceptible to the test samples except Mucor circinelloides. The zone of microbial growth inhibition of the samples was found in 10-40 mm range and the minimum inhibitory values ranged between 3.9 and 1000 µg/mL. The ethyl acetate fraction exhibited significant antimicrobial potential especially against P. aeruginosa, S. aureus and C. albicans where the diameters of inhibition zones were 40, 30 and 25mm, respectively and their corresponding MIC values were 3.9, 7.8 and 15.6 µg/mL, respectively. The UPLC-ESI-MS analysis of the samples revealed the tentative identification of various phenolic compounds mainly corilagin. A. altissima offers a perfect choice to be investigated as a natural and potent antimicrobial agent.

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LC-ESI-MS Analysis, Antitumor and Antiviral activities of Bosica senegalensis aqueous methanolic extract

Cited by 4 publications

References 14 publications

Integration of medicinal plants into the traditional system of medicine for the treatment of cancer in Sokoto State, Nigeria

Integration of medicinal plants into the traditional system of medicine for the treatment of cancer in Sokoto State, Nigeria

Bone Fracture-healing Properties and UPLC-MS Analysis of an Enriched Flavonoid Fraction from Oxystelma esculentum

UPLC-ESI-MS polyphenolic profiling and the antimicrobial potential of Ailanthus altissima (Mill.) Swingle leaves growing in Egypt

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