LC-ESI-MS/MS Phenolic Profile of Volutaria lippii (L.) Cass. Extracts and Evaluation of Their In Vitro Antioxidant, Antiacetylcholinesterase, Antidiabetic, and Antibacterial Activities

Abstract

Volutaria lippii (L.) Cass., an indigenous perennial herb from the Tunisian flora, belongs to the medicinally important genus Volutaria Cass. (Asteraceae) which comprises eighteen species widely distributed in the Irano-Turanian and Mediterranean Basin. In this study, five different extracts from Tunisian Volutaria lippii (L.) Cass. were evaluated for their in vitro antioxidant, antiacetylcholinesterase, antidiabetic, and antibacterial activities as well as for their total phenolic and flavonoid contents. The results indicated that the ethyl acetate and aqueous fractions have the highest levels in phenolic and flavonoid contents and showed remarkable antioxidant activities using DPPH (IC50= 11.50±0.57 and 28.81±1.35μg/mL, respectively), total antioxidant capacity (105.21±0.01 and 98.77±0.02 mg vitamin E/g extract, respectively), and reducing power (EC50= 55.40±2.00 and 66.65±1.40 μg/mL, respectively) methods. Furthermore, they exhibited noticeable antiacetylcholinesterase and antidiabetic activities and a moderate antibacterial effect when compared to that of standards. Principal component analysis allowed highlighting the ethyl acetate extract for its interesting acetylcholinesterase enzyme (AChE) and alpha-amylase activities and the aqueous fraction for its remarkably antibacterial activity, and their richness in phytochemical content. Interestingly, the LC-ESI-MS/MS analyses of both fractions allowed the identification of ten phenolic acids and eight flavonoids. The 3-O-caffeoylquinic and 3,4-di-O-caffeoylquinic acids constituted the most abundant components in the two fractions. Taken together, these findings demonstrated, for the first time, that Volutaria lippii (L.) Cass. is a potential source of biological active compounds which could be used in a wide range of fields, namely, nutrition and complementary pharmacological drug.

Role of Flavonoids in the Treatment of Iron Overload

“…This results in damage to DNA, proteins, and lipids in cells, leading to cancer, hypertension, hyperlipidemia, or atherosclerosis. Antioxidants can block lipid chain autoxidation and provide hydrogen to free radicals, especially lipid peroxides, thus forming stable free radicals that do not initiate or induce further lipid oxidation ( Ben Salah et al, 2019 ). The antioxidant properties of phytochemicals, such as flavonoids, may have a similar effect.…”

Identification and quantification of phenolic and fatty acid profiles in Pinus halepensis mill. seeds by LC‐ESI‐MS and GC: Effect of drying methods on chemical composition

“…The compound 4 MS 2 mass spectrums showed fragments at m/z 463 [M‐H‐rhamnosyl] − and 301[M‐H‐rhamnosyl‐glucosyl] − (Figure 3). These results revealed that compound 4 was a rutin (Ben Salah et al, 2019). In addition, compound 6 had a deprotonated molecule [M‐H] ‐ at m/z 447, and a strong fragment at m/z 285 suggested that this compound was the luteolin‐7‐ O ‐glucoside.…”

“…LC‐ESI‐MS analysis was performed in order to increase the nutritional value of P. halepensis Mill. The identification of the phenolic compounds was carried out by mass spectra, comparison with reference compounds and with literature data (Rigane et al., 2011; Rigane et al., 2013; Boukhris et al., 2013; Ben Salah et al, 2019; Ben Hmed et al., 2020). Table 3 illustrates all the identified peaks with retention times, the pseudomolecular ions, as well as the concentration of each identified phenolic compound.…”

Optimum Conditions and LC-ESI-MS Analysis of Phenolic Rich Extract from Eucalyptus marginata L. under Maceration and Ultrasound-Assisted Extraction Methods Using Response Surface Methodology

“…e chemical composition of the two Eucalyptus marginata L. extracts obtained after maceration and ultrasound-assisted extraction parameters was analyzed with LC-ESI-MS in the negative mode (Figure 5). Based on the mass spectra and comparison with reference compounds and with literature data [4,6,7,[37][38][39], the detected compounds were classified as phenolic acids such as quinic, gallic, protocatechuic, pcoumaric, salviolinic, trans-ferulic, and trans-cinnamic acids and flavonoids such as hyperoside, rutin, quercetrin, naringin, quercetin, and naringenin. Retention times, pseudomolecular ions, and the concentration of each identified phenolic compound are given in Table 4.…”

“…Eucalyptus marginata is easily recognized by its flowers and fruits. Many research studies showed that the antioxidant activity, which is generally attributed with the interesting phenolic composition, allows this plant to be widely used in food and pharmaceutical industries [1][2][3][4][5][6][7]. Previous work showed that Eucalyptus is like any medicinal plant present an original chemical composition.…”