LC Method for the Determination of the Stability of Levetiracetam Drug Substance under Stressing Conditions

Saravanan, G.; Jyothi, G.; Suresh, Y.; Annerao, A.; Ramakrishna, M.; Reddy, M. Yogeshwar; Ravibabu, B.

doi:10.1365/s10337-007-0472-7

Cited by 14 publications

(4 citation statements)

References 7 publications

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“…The chromatographic parameters were adapted from a stability indicating assay previously published by Saravanan et al 2 A YMC C18 column e was used and maintained at 30°C.…”

Section: High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Stability of levetiracetam oral solution repackaged in oral plastic syringes

Prohotsky

Hughes

Zhao

2014

American Journal of Health-System Pharmacy

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“…The chromatographic parameters were adapted from a stability indicating assay previously published by Saravanan et al 2 A YMC C18 column e was used and maintained at 30°C.…”

Section: High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Stability of levetiracetam oral solution repackaged in oral plastic syringes

Prohotsky

Hughes

Zhao

2014

American Journal of Health-System Pharmacy

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“…Stability studies suggest proper formulations, design manufacturing processes, and selecting proper storage condition and packaging for the drug product. Furthermore, it helps in establishing shelf life of product [6][7][8][9] . All these make it highly important to have a stability indicating method in hand.…”

Section: Introductionmentioning

confidence: 99%

An Improved Rp-HPLC Method for the Quantitative Determination and Validation of Levetiracetam in Bulk and Pharmaceutical Formulation

Ravisankar¹,

Niharika²,

P³

2015

Int. Res. J. Pharm.

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The present study depicts the development of a validated RP-HPLC method for the determination of the Levetiracetam in pharmaceutical tablet dosage form. RP-HPLC method was developed by utilizing Welchrom C18 Column (250 x 4.6 mm, 5µ), Shimadzu LC-20AT Prominence Liquid Chromatograph. The mobile phase composed of 10 mM Phosphate buffer: acetonitrile (50:50 v/v) (pH-3.0, adjusted with triethylamine). The flow rate was set to 1.0 ml/min with the responses measured at 243.2 nm using Shimadzu SPD-20A Prominence UV-Visible detector. The retention time of Levetiracetam was found to be 3.030 minutes. Linearity was established for Levetiracetam in the range of 10-50 µg/ml with correlation coefficient 0.9998. The LOD and the LOQ for Levetiracetam found to be 0.468 μg/ml and 1.421 μg/ml respectively. The amount of Levetiracetam present in the formulation was found to be 99.56 %. None of the excipients interfered with the analyte of interest. Considering all the results of validation parameters simplicity of the method and the cost effectiveness of the overall procedure, it is possible to conclude that the developed method can be suitable for the regular quality control determination of Levetiracetam in bulk as well as pharmaceutical dosage form.

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“…Literature assessment disclosed that levetiracetam has been estimated by various methods in pure and pharmaceutical dosage forms including HPLC-UV [ 6 – 11 ] and HPLC-MS [ 12 , 13 ], while piracetam by HPLC, micellar electrokinetic chromatography, thin layer densitometry and capillary electrophoresis, and so forth [ 14 – 25 ].…”

Section: Introductionmentioning

confidence: 99%

Development of New Method for Simultaneous Analysis of Piracetam and Levetiracetam in Pharmaceuticals and Biological Fluids: Application in Stability Studies

Siddiqui

Sher

Shafi

et al. 2014

BioMed Research International

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RP-HPLC ultraviolet detection simultaneous quantification of piracetam and levetiracetam has been developed and validated. The chromatography was obtained on a Nucleosil C18 column of 25 cm × 0.46 cm, 10 μm, dimension. The mobile phase was a (70 : 30 v/v) mixture of 0.1 g/L of triethylamine and acetonitrile. Smooth flow of mobile phase at 1 mL/min was set and 205 nm wavelength was selected. Results were evaluated through statistical parameters which qualify the method reproducibility and selectivity for the quantification of piracetam, levetiracetam, and their impurities hence proving stability-indicating properties. The proposed method is significantly important, permitting the separation of the main constituent piracetam from levetiracetam. Linear behavior was observed between 20 ng/mL and 10000 ng/mL for both drugs. The proposed method was checked in bulk drugs, dosage formulations, physiological condition, and clinical investigations and excellent outcome was witnessed.

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LC Method for the Determination of the Stability of Levetiracetam Drug Substance under Stressing Conditions

Cited by 14 publications

References 7 publications

Stability of levetiracetam oral solution repackaged in oral plastic syringes

Stability of levetiracetam oral solution repackaged in oral plastic syringes

An Improved Rp-HPLC Method for the Quantitative Determination and Validation of Levetiracetam in Bulk and Pharmaceutical Formulation

Development of New Method for Simultaneous Analysis of Piracetam and Levetiracetam in Pharmaceuticals and Biological Fluids: Application in Stability Studies

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