LC-MS based metabolite profiling reveals hydroxylcinnamoyl conjugation as a discriminatory chemical factor between two closely related Coccinia species

Nengovhela, Ndamulelo; Mbedzi, Dakalo Terrence; Ndhlala, A.R.; Mathomu, Lutendo Michael; Mhlongo, Msizi I.; Madala, Ntakadzeni E.

doi:10.1016/j.sajb.2021.04.034

Cited by 3 publications

(1 citation statement)

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“…Recently, it has been shown that chemical modifications associated with cinnamic acid molecule are essential in ensuring the qualitative outcomes of UV-induced geometrical isomerization in plants extracts (Moyo et al, 2022). For instance, cinnamic acid can be conjugated to various organic acids (Nengovhela et al, 2022), such as quinic acid (Ncube et al, 2014), citric/isocitric acid (Masike et al, 2017) and glucaric acid (Ramphinwa et al, 2022a(Ramphinwa et al, , 2022b. Interestingly, caffeoylquinic acid and other hydroxyl-cinnamic acid derivatives have been shown to have anti-HIV-1 INT enzyme activity (Makola et al, 2016a) and, as such, agricultural means to grow plants with enhance levels or means to diversify these compounds should be developed.…”

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confidence: 99%

Conjugation of Glucaric Acid in Comparison to Quinic Acid by Caffeic Acid Allows for Enhanced Metabolite Diversification in Bush Tea (<i>Athrixia phylicoides</i> DC.) Extracts Post UV Light Exposure

RAMPHINWA,

MCHAU,

MADALA

et al. 2023

Environ. Control Biol.

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The cultivation of bush tea (Athrixia phylicoides DC.) has been shown to be affected by light availability which has encouraged the use of different net shading to filter the amount of light on the plant. The objective of this study was to enhance the levels of caffeoyl-D-glucaric acid derivatives through UV induced geometrical isomerization. Bush tea methanolic leaf extracts were placed under UV light lamp at 254 nm for the duration of 24 h and new metabolites which formed due to UV exposure were detected through ultra-high-liquid chromatography quadrupole time-of-fight mass spectrometry (UHPLC-QTOF-MS). Hydroxyl-cinnamic acids (HCAs) derivatives have been shown to undergo photoisomerization during post ultra-violet (UV) light exposure, evidenced by the emergence of photo-isomers.

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