LC‐MS Bioanalysis of ACYL Glucuronides

Zhou, Jiaqi; Li, Feng Frank; Duggan, Jeffrey X.

doi:10.1002/9781118671276.ch35

Cited by 5 publications

(4 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Few investigations on the stability of bile samples according to storage conditions have been conducted. To prevent hydrolysis of glucuronoconjugated metabolites, the sample may be mildly acidified by adding a buffer (such as 1 M ammonium acetate pH 4.0/5.5 or sodium acetate 0.1 M pH 5) or acid (acetic or hydrochloric) at the time of collection 23, 24 . Melo et al, 19,25 investigated the stability of four benzodiazepines (lorazepam, estazolam, ketalozam, and chlordiazepoxide) in bile for up to six months at 8 o C, 2 o C, +4 o C, and +25 o C with or without the addition of sodium fluoride (NaF) as a preservative.…”

Section: Resultsmentioning

confidence: 99%

Impact of Sample Storage Conditions on Forensic Toxicology Analysis – A Review

2023

View full text Add to dashboard Cite

In forensic toxicology, the preservation of matrices is a crucial stage in the analysis that affects the accuracy and reliability of the findings. To reduce interference and improve the findings’ interpretation, specimens should be maintained in the right environment. For analytical objectives in forensic toxicology, we aim to characterize the modalities of preservation of the distinct matrices for each incriminated toxin. A comprehensive literature search in both English and French languages was conducted using the keywords "forensic samples preservation" to find pertinent papers in the PubMed and ScienceDirect databases. The results were then carefully examined. The preservation method based on the biological matrix and the required harmful substances was the major focus of the investigation. This bibliographic research’s objective is to understand the sample preservation methods used in diverse studies as well as each method’s limitations in light of the biological matrix and xenobiotics being investigated. The mode and duration of conservation depend closely on the matrix and the toxins to be sought. Certain hazardous compounds' chemical stability in biological matrices is influenced by storage conditions compliance. Therefore, it is apparent that forensic scientists would benefit from knowledge of the sampling and storage procedures for substances suspected of posing a threat. Peer Review History: Received: 17 December 2022; Revised: 9 February 2023; Accepted: 5 March, Available online: 15 March 2023 Academic Editor: Dr. Marwa A. A. Fayed, University of Sadat City, Egypt, maafayed@gmail.com Received file: Reviewer's Comments: Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewers: Dr. Sally A. El-Zahaby, Pharos University in Alexandria, Egypt, sally.elzahaby@yahoo.com Dr. Nuray Arı, Ankara University, Turkiye, ari@ankara.edu.tr Dr. Bilge Ahsen KARA, Ankara Gazi Mustafa Kemal Hospital, Turkey, ahsndkyc@gmail.com Similar Articles: STORAGE EFFECT ON THE GC-MS PROFILING AND ANTIOXIDANT ACTIVITIES OF ESSENTIAL OILS FROM LEAVES OF ANNONA SQUAMOSA L.

show abstract

Section: Resultsmentioning

confidence: 99%

Impact of Sample Storage Conditions on Forensic Toxicology Analysis – A Review

2023

View full text Add to dashboard Cite

show abstract

“…Two mass transitions for the analyte may be included in the acquisition method, which will provide an alternative if matrix effects or interference is observed in one transition. For a carboxylic acid containing drug, monitoring acyl glucuronide metabolite in addition of the parent compound helps to identify potential erroneous results [9,[11][12]. To minimize carry-over, the injection sequence should be arranged so that the samples with the same time points are injected together and samples with a lower expected concentration should be injected before those with a higher expected concentration.…”

Section: Data Acquisitionmentioning

confidence: 98%

“…Finally, incorporating precautionary sample pre-treatment based chemical structures and ADME screening results may be critical for certain compounds to ensure data quality. For example, plasma samples of carboxylic acid containing compounds should be pre-treated with acid to prevent the back conversion of acyl glucuronide metabolites to the dosed compound [9].…”

Section: Sample Preparationmentioning

confidence: 99%

Best Practices for Discovery Bioanalysis: Balancing Data Quality and Productivity

Ho¹

2014

Bioanalysis

View full text Add to dashboard Cite

“…Therefore, to accurately quantify either an acyl glucuronide or its parent aglycone, it is essential to prevent acyl glucuronide hydrolysis. Acyl glucuronides hydrolysis is pH dependent so in general, higher pH accelerates hydrolysis reaction (Zhou, Feng, & Duggan, ). Therefore, controlling sample pH becomes important while measuring the acyl glucuronide.…”

Section: Analytical Measurement Of Acyl Glucuronidementioning

confidence: 99%

Bioanalytical challenges and strategies for accurately measuring acyl glucuronide metabolites in biological fluids

Patel

2019

Biomedical Chromatography

View full text Add to dashboard Cite

Bioanalysis of unstable compounds such as acyl glucuronide metabolites represents a great analytical challenge owing to poor analyte stability in biological matrices. The primary goal for bioanalytical assay development is to minimize the breakdown of acyl glucuronide metabolite into its parent aglycone during sample collection, transportation, storage and analysis. Samples need to be stabilized ex vivo immediately after sample collection to minimize potential breakdown and thus to ensure accurate concentration measurement of both acyl glucuronide metabolite and its parent aglycone. In this review paper, formation of acyl glucuronide metabolites, the importance of establishing acyl glucuronide exposure measurement and safety coverage, optimization of sample pretreatment to stabilize the acyl glucuronide metabolites, current analytical strategy of assaying them as well as considerations for regulatory filings are discussed. It is important to identify acyl glucuronide metabolites that are capable of undergoing hydrolysis and pH‐dependent intra‐molecular migration as well as covalently binding to plasma and tissue proteins which can cause toxicity in vivo in the early stages of drug development. Carefully planning analytical experiments, identifying structures of acyl glucuronides and monitoring their concentrations in early drug development can help assess the risks associated with their exposures and potentially predict their concentrations in human circulation.

show abstract

LC‐MS Bioanalysis of ACYL Glucuronides

Cited by 5 publications

References 49 publications

Impact of Sample Storage Conditions on Forensic Toxicology Analysis – A Review

Impact of Sample Storage Conditions on Forensic Toxicology Analysis – A Review

Best Practices for Discovery Bioanalysis: Balancing Data Quality and Productivity

Bioanalytical challenges and strategies for accurately measuring acyl glucuronide metabolites in biological fluids

Contact Info

Product

Resources

About