LC‐MS Bioanalysis of Interconvertible Compounds

Merbel, Nico van de

doi:10.1002/9781118671276.ch40

Cited by 3 publications

(1 citation statement)

References 52 publications

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“…The third quadrupole serves to produce a mass spectrum from the product ions. LC‐MS/MS is highly accurate, precise, and can handle highly complex samples, but lacks sensitivity [89] …”

Section: Literature and Discussionmentioning

confidence: 99%

Pharma‐molecule Transport across Bacterial Membranes: Detection and Quantification Approaches by Electrochemistry and Bioanalytical Methods

et al. 2021

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Antibiotic resistance is a significant challenge encountered by healthcare systems on a global scale. Knowledge about membrane transport of antibiotics and other pharmacologically relevant molecules in bacteria is crucial towards understanding and overcoming antibiotic resistance, as drug resistance often depends on drug transport. This comprehensive literature review discusses the detection and quantification of membrane transport of pharma‐molecules in bacteria and highlights the importance of molecule transport to antibiotic resistance. This review emphasizes electrochemical and electrophysiological methods of detection and quantification. The results of this literature review reveal a substantial diversity in methods and types of quantitative information collected.

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“…The third quadrupole serves to produce a mass spectrum from the product ions. LC‐MS/MS is highly accurate, precise, and can handle highly complex samples, but lacks sensitivity [89] …”

Section: Literature and Discussionmentioning

confidence: 99%

Pharma‐molecule Transport across Bacterial Membranes: Detection and Quantification Approaches by Electrochemistry and Bioanalytical Methods

et al. 2021

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Stability of energy metabolites—An often overlooked issue in metabolomics studies: A review

et al. 2015

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Recent advances in analytical chemistry have set the stage for metabolite profiling to help understand complex molecular processes in physiology. Despite ongoing efforts, there are concerns regarding metabolomics workflows, since it has been shown that internal (enzyme activity, blood contamination, and the dynamic nature of metabolite concentrations) as well as external factors (storage, handling, and analysis method) may affect the metabolome profile. Many metabolites are intrinsically instable, particularly some of those associated with central carbon metabolism. While enzymatic conversions have been studied in great detail, nonenzymatic, chemical conversions received comparatively little attention. This review aims to give an in-depth overview of nonenzymatic energy metabolite degradation/interconversion chemistry focusing on a selected range of metabolites. Special attention will be given to qualitative (degradation pathways) as well as quantitative aspects, that may affect the acquisition of accurate data in the context of metabolomics studies. Problems related to the use of isotopically labeled internal standards hindering the quantitative analysis of common metabolites will be presented with an experimental example. Finally, general conclusions and perspectives are given.

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Development and Validation of Bioanalytical Methods to Support Investigations of AZD9496 in the Clinic

et al. 2020

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Aim: AZD9496 is an oral nonsteroidal, potent and selective antagonist and degrader of ER-α. Two major active metabolites (M3 and M5 as diastereomers) were identified in humans. Methodology/results: Multianalyte, sensitive LC–MS/MS method in human plasma was developed and validated that overcame the challenges encountered. The method demonstrated acceptable precision, accuracy and selectivity for AZD9496 and two major metabolites. Incurred sample reanalysis was acceptable from evaluation in clinical studies, indicating adequate reproducibility. In addition, a urine method for AZD9496 was also developed and validated. Conclusion: Robust and sensitive LC–MS/MS assays for the quantitation of AZD9496 and two diastereomeric metabolites in human plasma and AZD9496 in human urine have been validated and successfully applied to clinical studies.

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LC‐MS Bioanalysis of Interconvertible Compounds

Cited by 3 publications

References 52 publications

Pharma‐molecule Transport across Bacterial Membranes: Detection and Quantification Approaches by Electrochemistry and Bioanalytical Methods

Pharma‐molecule Transport across Bacterial Membranes: Detection and Quantification Approaches by Electrochemistry and Bioanalytical Methods

Stability of energy metabolites—An often overlooked issue in metabolomics studies: A review

Development and Validation of Bioanalytical Methods to Support Investigations of AZD9496 in the Clinic

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