LC-MS/MS Analysis and Hepatoprotective Activity of Artichoke (Cynara scolymusL.) Leaves Extract against High Fat Diet-Induced Obesity in Rats

Abstract

Cynara scolymus L. (Artichoke) has been used for the treatment of metabolic disorders. The purpose of the present study was to investigate the hepatoprotective effect of Cynara scolymus leaves extract against a high fat diet (HFD) induced rats. This study investigated the most abundant phenolic compounds rich Cynara scolymus leaves extract and it is antihypercholesterolemic and antioxidative effects in vivo. The hypercaloric high fat diet (HFD) was treated with 200 mg/kg and 400 mg/kg of ethanol extract (EEA) from leaves of Cynara and atorvastatin (ATOR) (10 mg/kg/day) during an 8-week period. Lipid profile was measured and oxidative stress systematic in hepatic tissue was determined. Our data revealed that HFD-induced hepatic dysfunction manifested by significant abnormal levels of AST, ALT, ALP, LDH, and OCT was accompanied by increasing levels of oxidative stress biomarker (ROS, MDA, and AOPP) while decreasing in antioxidant status. Coadministration of EEA significantly reduced serum lipid profile and hepatic disorders which was confirmed to be histological by reducing the fatty liver deposition in hepatic lobule. These findings suggest that Cynara leaves exert antiobesity and antioxidant liver effects in HFD-induced obese rats.

Effect of the Diets Supplemented with Artichoke and Shrimp by-Products on Obese Rats

“…However, a significant reduction was noted after 8 weeks for all treated groups in the white blood cells (WBC) and hematocrit (HCT) when compared to the positive group. Administration of artichoke leaves extract (200-400 mg/kg) for one-month rats increased the levels of WBC, RBC, Hb, MCV, MCH, and platelets to near normal values (p ≤ 0.001) in alloxan induced diabetic obesity rats (Salem et al, 2019).…”

Metabolic and Anti-Inflammatory Protective Properties of Human Enriched Serum Following Artichoke Leaf Extract Absorption: Results from an Innovative Ex Vivo Clinical Trial

“…In a mouse model of cirrhosis, daily gavage for 10 days with ALE at a dose of 1.6 g/kg body weight limits hepatic tissue degeneration [ 5 ]. In diabetic rats, the daily administration of ALE at 400 mg/kg body weight for 8 weeks, exerts anti-hyperglycemic, antioxidant and lipid-lowering effects [ 11 , 15 ]. These doses of 400 mg/kg/day in rats and 1.6 g/kg/day in mice for 10 to 56 days correspond to 8 to 15 g/day in humans according to the metabolic weight conversion table.…”

“…Three healthy volunteers who fasted for 12 h were given at once 8 g of an artichoke leaf extract (ALE). The dose was set according to validated preclinical and clinical data [ 5 , 11 , 14 , 15 , 16 , 17 ]. The ALE extract was given dissolved in 200 mL of water.…”

“…In patients with diagnosed NAFLD, ALE treatment (600 mg daily for 9 weeks) reduces liver size, serum total cholesterol and triglyceride concentrations [ 10 ]. In a rat model of a high-fat diet, ALE limits hepatic disorders by reducing the fatty liver deposition in the hepatic lobule [ 11 ].…”

تأثیر مستخلص أوراق الخرشوف ضد إصابة الکلى ببرومات البوتاسیوم فی الفئران وإمکانیة إضافته فی بعض السلطات الصالحة للأکل

“…The nephrotoxicity caused by KBrO3 has been attributed to its ability to trigger reaction production of oxygen species (ROS), lipid peroxidation and 8-hydroxyguanosine modification in renal DNA (Spassova et al, 2015). Cynara scolymus is a perennial herbaceous plant belonging to family Asteraceae and usually known by the name of artichoke has been reported to have anti oxidative anti diabetic, anti microbial, cholesterol biosynthesis inhibitor, nephroprotective, antiinflammatory properties (Salem et al, 2019). In addition, it offers protection against degenerative changes such as cancer.…”