LC-MS/MS analysis of carboxymethylated and carboxyethylated phosphatidylethanolamines in human erythrocytes and blood plasma

Shoji, Naoki; Nakagawa, Kiyotaka; Asai, Akira; Fujita, Ikuko; Hashiura, Aya; Nakajima, Yasushi; Oikawa, Shinichi; Miyazawa, Teruo

doi:10.1194/jlr.d004564

Cited by 50 publications

(55 citation statements)

References 34 publications

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“…While prior studies have shown the elevated levels of Amadori-PEs in plasma of diabetic patients and in different organs of diabetic animal models ( 38,39 ), ours is the fi rst report establishing a relationship between glycated PEs and DN. Glycated PEs have been shown to alter the structure and stability of cell membrane proteins and promote lipid peroxidation ( 40,69 ), thus exhibiting pathogenic potential.…”

Section: Nonenzymatic Modifi Cation Of Pe By Glucose Is Increased In mentioning

confidence: 73%

“…Nonenzymatic adduction of glucose to aminophospholipids has been shown to increase in diabetic human plasma and animal tissues including kidney ( 38,39 ). However, with the exception of diabetic atherosclerotic lesions ( 40 ), the role of glycated lipids in diabetic complications has not been investigated.…”

Section: Nonenzymatic Modifi Cation Of Pe By Glucose Is Increased In mentioning

confidence: 99%

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Diabetic nephropathy induces alterations in the glomerular and tubule lipid profiles

Grove

Voziyan

Spraggins

et al. 2014

Journal of Lipid Research

104

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Section: Nonenzymatic Modifi Cation Of Pe By Glucose Is Increased In mentioning

confidence: 73%

Section: Nonenzymatic Modifi Cation Of Pe By Glucose Is Increased In mentioning

confidence: 99%

Diabetic nephropathy induces alterations in the glomerular and tubule lipid profiles

Grove

Voziyan

Spraggins

et al. 2014

Journal of Lipid Research

104

View full text Add to dashboard Cite

“…Besides Amadori-PE, we found recently that advanced glycation end products of PE are also present in human blood. 89,90) In addition, lipid glycation impairs the flavor, color, and nutritive value of foods containing large amounts of Amadori-PE, which include infant formula and chocolate. lack of a lipid glycation model for inhibitor screening.…”

Section: Analysis Of Lipid Glycation Products In Blood and Tissues Ofmentioning

confidence: 99%

Studies Targeting α-Glucosidase Inhibition, Antiangiogenic Effects, and Lipid Modification Regulation: Background, Evaluation, and Challenges in the Development of Food Ingredients for Therapeutic Purposes

Nakagawa

2013

Bioscience, Biotechnology, and Biochemistry

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“…Lipid classes were then assessed based on position on the 2D-TLC, according to previous studies (Simionato et al, 2013), adding a DGTS standard that was not used in this previous study. Carboxymethyl phosphatidylethanolamine was identified by mass spectrometry as described by Shoji et al (2010). Glycerolipids were then scraped off from the 2D-TLC plates for further analyses.…”

Section: Glycerolipid Analysismentioning

confidence: 99%

Light Remodels Lipid Biosynthesis in Nannochloropsis gaditana by Modulating Carbon Partitioning between Organelles

et al. 2016

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The seawater microalga Nannochloropsis gaditana is capable of accumulating a large fraction of reduced carbon as lipids. To clarify the molecular bases of this metabolic feature, we investigated light-driven lipid biosynthesis in Nannochloropsis gaditana cultures combining the analysis of photosynthetic functionality with transcriptomic, lipidomic and metabolomic approaches. Light-dependent alterations are observed in amino acid, isoprenoid, nucleic acid, and vitamin biosynthesis, suggesting a deep remodeling in the microalgal metabolism triggered by photoadaptation. In particular, high light intensity is shown to affect lipid biosynthesis, inducing the accumulation of diacylglyceryl-N,N,N-trimethylhomo-Ser and triacylglycerols, together with the up-regulation of genes involved in their biosynthesis. Chloroplast polar lipids are instead decreased. This situation correlates with the induction of genes coding for a putative cytosolic fatty acid synthase of type 1 (FAS1) and polyketide synthase (PKS) and the down-regulation of the chloroplast fatty acid synthase of type 2 (FAS2). Lipid accumulation is accompanied by the regulation of triose phosphate/inorganic phosphate transport across the chloroplast membranes, tuning the carbon metabolic allocation between cell compartments, favoring the cytoplasm, mitochondrion, and endoplasmic reticulum at the expense of the chloroplast. These results highlight the high flexibility of lipid biosynthesis in N. gaditana and lay the foundations for a hypothetical mechanism of regulation of primary carbon partitioning by controlling metabolite allocation at the subcellular level.

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LC-MS/MS analysis of carboxymethylated and carboxyethylated phosphatidylethanolamines in human erythrocytes and blood plasma

Cited by 50 publications

References 34 publications

Diabetic nephropathy induces alterations in the glomerular and tubule lipid profiles

Diabetic nephropathy induces alterations in the glomerular and tubule lipid profiles

Studies Targeting α-Glucosidase Inhibition, Antiangiogenic Effects, and Lipid Modification Regulation: Background, Evaluation, and Challenges in the Development of Food Ingredients for Therapeutic Purposes

Light Remodels Lipid Biosynthesis in Nannochloropsis gaditana by Modulating Carbon Partitioning between Organelles

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