LC-MS/MS analysis of novel ovatoxin isomers in several Ostreopsis strains collected in Japan

Suzuki, Toshiyuki; Watanabe, Ryuichi; Uchida, Hajime; Matsushima, Ryoji; Nagai, Hiroshi; Yasumoto, Takeshi; Yoshimatsu, Takamichi; Sato, Shinya; Adachi, Masao

doi:10.1016/j.hal.2012.08.002

Cited by 45 publications

(40 citation statements)

References 29 publications

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“…The acquired data were analyzed using Agilent MassHunter software. The LC/MS comparison between ovatoxin‐a and OVTa‐IK2 was carried out following the published method for ovatoxins …”

Section: Methodsmentioning

confidence: 99%

Structural elucidation of palytoxin analogs produced by the dinoflagellate Ostreopsis ovata IK2 strain by complementary use of positive and negative ion liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Uchida

Taira

Yasumoto

2013

Rapid Comm Mass Spectrometry

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“…The acquired data were analyzed using Agilent MassHunter software. The LC/MS comparison between ovatoxin‐a and OVTa‐IK2 was carried out following the published method for ovatoxins …”

Section: Methodsmentioning

confidence: 99%

Structural elucidation of palytoxin analogs produced by the dinoflagellate Ostreopsis ovata IK2 strain by complementary use of positive and negative ion liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Uchida

Taira

Yasumoto

2013

Rapid Comm Mass Spectrometry

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“…The toxin scenario becomes even more complicated considering toxin profiles of Ostreopsis spp. strains from Japan that produce a number of different isomers of ovatoxins, namely ovatoxins AC [16] and ovatoxins IKA2 [17]. Such findings suggest that toxin profiles may be a valuable feature to characterize new strains of O. cf.…”

Section: Introductionmentioning

confidence: 96%

The novel ovatoxin-g and isobaric palytoxin (so far referred to as putative palytoxin) from Ostreopsis cf. ovata (NW Mediterranean Sea): structural insights by LC-high resolution MSn

et al. 2014

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Blooms of the benthic dinoflagellate Ostreopsis cf. ovata are a concern in the Mediterranean Sea, since this species produces a wide range of palytoxin-like compounds listed among the most potent marine toxins. This study focused on two analogs of palytoxin found in cultures of six strains of O. cf. ovata isolated from the south of Catalonia (NW Mediterranean Sea). In addition to some already known ovatoxins, our strains produced two minor compounds, ovatoxin-g and the so far called putative palytoxin, whose structures had not been elucidated before. Insufficient quantity of these compounds impeded a full nuclear magnetic resonance (NMR)-based structural elucidation; thus, we studied their structure in crude algal extracts through liquid chromatography-electrospray ionization high-resolution mass spectrometry(n) (LC-ESI-HRMS(n)) in positive ion mode. Under the used MS conditions, the molecules underwent fragmentation at many sites of their backbone and a large number of diagnostic fragment ions were identified. As a result, tentative structures were assigned to both ovatoxin-g and the putative palytoxin, the latter being identified as a palytoxin isomer and re-named as isobaric palytoxin.

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“…PLTX and 42-hydroxy-palytoxin (42-OH-PLTX) were identified from different species of zoanthids, together with other minor toxins [7][8][9]. PLTX and ovatoxins (from -a to -h, and some isomers) were detected in different strains of Ostreopsis ovata [10][11][12][13][14]. In O. siamensis ostreocin-d was described as the major toxin [15], while mascarenotoxins (-a and -b) were extracted from O. mascarenensis [6].…”

Section: Introductionmentioning

confidence: 99%

Detection of palytoxin-like compounds by a flow cytometry-based immunoassay supported by functional and analytical methods

Fraga-Corral

Vilariño

Louzao

et al. 2016

Analytica Chimica Acta

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LC-MS/MS analysis of novel ovatoxin isomers in several Ostreopsis strains collected in Japan

Cited by 45 publications

References 29 publications

Structural elucidation of palytoxin analogs produced by the dinoflagellate Ostreopsis ovata IK2 strain by complementary use of positive and negative ion liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Structural elucidation of palytoxin analogs produced by the dinoflagellate Ostreopsis ovata IK2 strain by complementary use of positive and negative ion liquid chromatography/quadrupole time‐of‐flight mass spectrometry

The novel ovatoxin-g and isobaric palytoxin (so far referred to as putative palytoxin) from Ostreopsis cf. ovata (NW Mediterranean Sea): structural insights by LC-high resolution MSn

Detection of palytoxin-like compounds by a flow cytometry-based immunoassay supported by functional and analytical methods

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