LC-MS/MS analysis of palytoxin analogues in blue humphead parrotfish<i>Scarus ovifrons</i>causing human poisoning in Japan

Suzuki, Toshiyuki; Watanabe, Ryuichi; Matsushima, Ryoji; Ishihara, Kenji; Uchida, Hajime; Kikutsugi, Saori; Harada, Tomoko; Nagai, Hiroshi; Adachi, Masao; Yasumoto, Takeshi; Murata, Masakazu

doi:10.1080/19440049.2013.790085

Cited by 18 publications

(9 citation statements)

References 21 publications

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“…Haff disease must be differentiated from the rhabdomyolysis syndromes caused by palytoxins and other marine toxins with palytoxin-like effects found in certain saltwater fish [30,31,32]. The toxin responsible for Haff disease is found in freshwater crayfish and fish.…”

Section: Discussionmentioning

confidence: 99%

The Emergence and Epidemiology of Haff Disease in China

Chan

2016

Toxins

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Haff disease is a rare syndrome of unexplained myalgia and rhabdomyolysis occurring within 24 h of consumption of certain types of cooked freshwater fish or crustacean. It is caused by a yet unidentified heat-stable toxin. In the present review of published case studies and official press releases, the main objective is to report the emergence and epidemiology of Haff disease in China. Haff disease first occurred in Beijing in 2000 and in Lianzhou and Liannan, Guangdong Province in 2009. Subsequent outbreaks mostly occurred in the Jiangsu Province—Nanjing, Yangzhou, Huai’an, and Yancheng. Isolated outbreaks occurred in other cities since 2010—Shijiazhuang, Yueyang, Shanghai, Wuhu, Baoding, Shenzhen, and Hong Kong (imported cases from Shenzhen). Outbreaks occurred predominately in the summer. Crayfish accounted for almost all the outbreaks. Two large outbreaks occurred in Lianzhou and Liannan in 2009 (n = 54) after eating pomfrets and in Nanjing in 2010 (n = 42) after eating crayfish. Other reports or outbreaks involved only 1–9 subjects (median 2 subjects). Variability in individual susceptibility and attack rates were noted, with many subjects remaining asymptomatic despite sharing the same seafood meal as the index cases. Adults were predominately involved. Symptoms occurred within 3–20 h of seafood ingestion, including myalgia, weakness, and, less frequently, nausea, vomiting, abdominal pain, and diarrhea. Myalgia and muscle weakness should normally subside within 2–3 days. Serum creatine phosphokinase became normal within 5–6 days. Abnormal renal function was uncommon. Serious complications (renal failure, multi-organ failure, and prolonged myopathy) and death were rare. In any subjects with unexplained myalgia and rhabdomyolysis, seafood consumption should be included in the history. All suspected cases of Haff disease, including milder presentations, should be reported to public health authorities.

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Section: Discussionmentioning

confidence: 99%

The Emergence and Epidemiology of Haff Disease in China

Chan

2016

Toxins

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“…with C 18 [23] and particularly Gemini C 18 [9,15,16,24,25], C 8 sorbents [26] or Hydrophilic Interaction Liquid Chromatography (HILIC) [27,28]. These columns were suitable for OVTX identification and quantification, in association with MS detection, but not sufficiently efficient for complete separation and purification of OVTXs.…”

Section: Most Authors Have Used Reversed Phase Chromatography To Analmentioning

confidence: 99%

Characterization of ovatoxin-h, a new ovatoxin analog, and evaluation of chromatographic columns for ovatoxin analysis and purification

Brissard

Hervé

Sibat

et al. 2015

Journal of Chromatography A

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The presence of Ostreopsis cf. ovata on the Mediterranean coast represents a serious concern to human health due to production of toxins - putative palytoxin and ovatoxins (ovatoxin-a, -b, -c, -d, -e, -f and -g). However, purified ovatoxins are not widely available and their toxicities are still unknown. In the present study, we report on HR LC-MS/MS analysis of a French O. cf. ovata strain (IFR-OST-0.3V) collected at Villefranche-sur-Mer (France) during a bloom in 2011. Investigation of this strain of O. cf. ovata cultivated in our laboratory by ultra-high performance liquid chromatography coupled to high resolution mass spectrometry (UHPLC-HRMS) confirmed the production of ovatoxins-a to -e and revealed the presence of a new ovatoxin analog, named ovatoxin-h. O. cf. ovata extracts were pre-purified by Sephadex LH-20 to obtain a concentrated fraction of ovatoxins (OVTXs). This method provided a recovery of about 85% of OVTXs and a cleanup efficiency of 93%. Different stationary phases were tested with this fraction of interest to elucidate the structure of the new OVTX congener and to obtain purified ovatoxins. Eight reversed phase sorbents were evaluated for their capacity to separate and purify ovatoxins. Among them Kinetex C18, Kinetex PFP and Uptisphere C18-TF allowed for best separations almost achieving baseline resolution. Kinetex C18 is able to sufficiently separate these toxins, allowing us to identify the toxins present in the extract purified by Sephadex LH-20, and to partly elucidate the structure of the new ovatoxin congener. This toxin possesses one oxygen atom less and two hydrogens more than ovatoxin-a. Investigations using liquid chromatography coupled to high resolution tandem mass spectrometry suggest that the part of the molecule where ovatoxin-h differs from ovatoxin-a is situated between C42 and C49. Uptisphere C18-TF was proposed as a first step preparative chromatography as it is able to separate a higher number of ovatoxins (especially ovatoxin-d and ovatoxin-e) and because it separates ovatoxins from unknown compounds, identified using full scan single quadrupole mass spectrometry. After pre-purification with Sephadex LH-20, purification and separation of individual ovatoxins was attempted using an Uptisphere C18-TF column. During recovery of purified toxins, problems of stability of OVTXs were observed, leading us to investigate experimental conditions responsible for this degradation.

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“…S. ovifrons is known to cause serious food poisoning. Although the causative agent of the poisoning remains controversial (Suzuki et al ., 2013; Taniyama et al ., 2003), it is believed that the toxin is accumulated in the body through the food chain because the toxicity of S. ovifrons differs among locations and seasons or years (Fusetani et al ., 1985; Taniyama et al ., 2003). This study revealed that S. ovifrons feeds mostly at depths <10 m, which suggests that the causative toxic organisms are likely to exist at shallower depths.…”

Section: Discussionmentioning

confidence: 99%

Diel vertical movements and feeding behaviour of blue humphead parrotfish Scarus ovifrons in a temperate reef of Japan

Gomi

Nakamura

Kanda³

et al. 2021

Journal of Fish Biology

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The feeding ecology of scarinine parrotfishes on tropical coral reefs has received considerable attention in the past few decades; nonetheless, relatively few studies have been conducted in high-latitude reefs. Among the Indo-Pacific Scarus species, Scarus ovifrons is unique, being largely restricted to the warm temperate waters of Japan.Nonetheless, there is very little information available on the feeding ecology of this species. In this study, the authors used acoustic telemetry to detect the diel vertical movement patterns of S. ovifrons, video survey to detect its feeding depths and substrata and focal follow survey and genetic analysis to identify algae composition on the feeding scars at Kashiwajima Island, southwestern Japan (32 46 0 N, 132 38 0 E).Acoustic telemetry revealed that S. ovifrons spent most of its time in shallow water (<10 m) during the day and slept in deeper water (10-15 m) at night. Video and focal follow surveys revealed that most fishes of various sizes regularly took bites on epilithic algae and detrital materials on rocky substrata at depths of <10 m, but large fishes (>40 cm total length) sometimes took bites directly on live corals (Acropora solitaryensis) at the 5 m depth zone where live tabular corals dominated the benthos.Molecular phylogenetic analyses revealed that epilithic algae collected from feeding scars were mainly composed of Rhodophyta, and coralline algae were less often targeted. Overall, this study revealed that S. ovifrons feeds mostly at depths <10 m, and the feeding algae substrata of the species are similar to those of tropical coral reef parrotfishes.

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LC-MS/MS analysis of palytoxin analogues in blue humphead parrotfishScarus ovifronscausing human poisoning in Japan

Cited by 18 publications

References 21 publications

The Emergence and Epidemiology of Haff Disease in China

The Emergence and Epidemiology of Haff Disease in China

Characterization of ovatoxin-h, a new ovatoxin analog, and evaluation of chromatographic columns for ovatoxin analysis and purification

Diel vertical movements and feeding behaviour of blue humphead parrotfish Scarus ovifrons in a temperate reef of Japan

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